LI Xiao-an,YIN Wei,YANG Guo-zhu,et al.Extraction Technology of Total Saponins from Alfalfa by Enzymatic Hydrolysis Method Assisted by Ultrasonic Technology[J].Northern Horticulture,2015,39(07):116-118.[doi:10.11937/bfyy.201507034]
超声辅助酶解法提取紫花苜蓿总皂苷工艺研究
- Title:
- Extraction Technology of Total Saponins from Alfalfa by Enzymatic Hydrolysis Method Assisted by Ultrasonic Technology
- Keywords:
- Medicago sativa L.; total saponins; enzymatic hydrolysis method assisted by ultrasonic technology; orthogonal method
- 文献标志码:
- A
- 摘要:
- 以“青大1号”紫花苜蓿（Medicago sativa L.）新品系青干草为试材，采用超声辅助酶解法提取总皂苷，对所得总皂苷提取液用石油醚、正丁醇进行2项萃取，紫外分光光度法测定总皂苷的含量。以单因素试验设计分别考察固液比（A）、超声时间（B）、超声温度（C）、超声功率（D）4个因素对总皂苷的粗总皂苷提取率的影响。以单因素试验结果为依据，采用4因素(固液比、超声时间、超声温度、超声功率)3水平正交实验设计进行提取工艺优化，以开发新的紫花苜蓿总皂苷资源。结果表明：超声法提取总皂苷最优工艺条件为A3B1C1D2，即固液比为1∶30 g/mL、超声时间20 min，超声温度20℃、超声功率360 W。
- Abstract:
- Taking new strain of alfalfa ‘Qingda No.1’ hay as raw material，the total saponins were extracted by enzymatic hydrolysis method assisted by ultrasonic technology，then total saponins extracted liquid with petroleum ether and n-butanol to two extraction，while determine the content of total saponins in Uv spectrophotometry.The ratio of solid to liquid（A），ultrasonic time（B）and ultrasonic temperature（C），ultrasonic power（D）as a single factor gradient test，based on single factor test results，using four factors (solid-liquid ratio，ultrasonic time，ultrasonic temperature，ultrasonic power) three levels orthogonal experiment design to optimize the extraction process,to develop new resources of alfalfa saponis.The results showed that the best ultrasonic method to extract the total saponins conditions was A3B1C1D2，the ratio of solid-liquid was 1∶30 g/mL，ultrasonic time was 20 miniutes，ultrasonic temperature was 20℃，ultrasonic power was 360 W.
参考文献/References:
[1]赵桂琴,慕平,张勃.紫花苜蓿基因工程研究进展[J].草业学报,2006,15(6):9-18. [2]Srinivasan K S,Sharnnthaka M C.Amino acid composition of some leaf protein concentrates progress in leaf research[M].Narendra Singh,1984:307-309. [3]Nowacka J,Oleszek W.Determination of alfalfa (Medicago saliva L.) saprations by high-performence liquid chromatography[J].Agr,1994,42:727-730. [4]王成章,王恬.饲料学[M].北京:中国农业出版社,2003. [5]李珍珠.人类的第七大营养素一膳食纤维[J].生物学教学,2004,29(10):33-34. [6]张清林,杨文宾.叶蛋白饲料生产技术[J].吉林畜牧兽医,2003(2):21-22. [7]何云,霍文颖,张海棠,等.紫花苜蓿的营养价值及其影响因素[J].安徽农业科学,2007,35(11):3243-3244. [8]Malinow M R,Connor W E,Mclaughlin P,et al.Effects of alfalfa saponins[J].Journal of Clinical Investigation,1981,67(1):156-162. [9]胡明,卢德勋,牛文艺,等.苜蓿皂苷对绵羊瘤胃内纤维物质降解动力学的影响[J].河南农业科学,2007(1):98. [10]丛学滋,李子行,秦孟根,等.苜蓿皂苷的降血胆固醇及减轻实验性动脉粥样硬化形成的作用[J].中国药理学通报,1998,4(5):69-76. [11]李波,魏广培.超声波提取苜蓿皂苷优化工艺的研究[J].食品科技,2012,37(4):167-168. [12]贾秀峰,李波,王佳莹.苜蓿皂苷提取方法的研究及皂苷含量的测定[J].食品研究与开发,2007,28(9):11-13,28.
相似文献/References:
[1]代惠萍,赵 桦,李军超,等.锌对紫花苜蓿生理生化特性的影响及积累能力研究[J].北方园艺,2014,38(10):50.
DAI Hui-ping,ZHAO Hua,LI Jun-chao,et al.Effects of Zn on the Physiological and Biochemical Characteristics and Accumulation Abilities of??Medikago sativa?L.[J].Northern Horticulture,2014,38(07):50.
[2]江超,夏阳,杨克强.盐胁迫下紫花苜蓿苗期根部生长及生理特性研究[J].北方园艺,2014,38(05):68.
JIANG Chao,XIA Yang,YANG Ke-qiang.Research on the Root Physiological Characteristics of Medicago sativa L.Seedling Under Salt Stress[J].Northern Horticulture,2014,38(07):68.
[3]韩凤波,陈静雅.不同采收期对香椿总黄酮和总皂苷含量的影响[J].北方园艺,2014,38(02):159.
HAN Feng-bo,CHEN Jing-ya.Effect of Harvest Season on Total Saponins Content and Total Flavone Content of Toona sinensis [J].Northern Horticulture,2014,38(07):159.
[4]朱华敏,李海梅,杨国锋,等.施肥对紫花苜蓿生产性能的影响[J].北方园艺,2013,37(14):174.
ZHU Hua-min,LI Hai-mei,YANG Guo-feng,et al.Effect of Fertilizer Application on Production Performance of Alfalfa[J].Northern Horticulture,2013,37(07):174.
[5]段亚楠,卢福荣,茹艺,等.紫花苜蓿组织培养高频再生体系的建立[J].北方园艺,2013,37(15):127.
DUAN Ya-nan,LU Fu-rong,RU Yi,et al.Establishment of High Frequency Regeneration System of Medicago sativa[J].Northern Horticulture,2013,37(07):127.
[6]孔 静,裴宗平,杜 旼,等.水分胁迫下AM真菌对紫花苜蓿生长及抗旱性的影响[J].北方园艺,2014,38(09):179.
KONG Jing,PEI Zong-ping,DU Min,et al.Effects of AM Fungi on Alfalfa Growth and Drought Resistance Under Water Stress[J].Northern Horticulture,2014,38(07):179.
[7]兰 光,李 扬,孙莉佳.枳椇总皂苷提取工艺研究[J].北方园艺,2013,37(20):129.
LAN Guang,LI Yang,SUN Jia-li.Study on the Extraction Technology of Total Saponins from Hovenia dulcis[J].Northern Horticulture,2013,37(07):129.
[8]覃勇荣,刘欣,黄光兵,等.无患子总皂苷提取工艺条件优化及天然洗涤剂研制[J].北方园艺,2013,37(16):143.
QINYong-rong,LIUXin,HUANGGuang-bing,et al.OptimizationofExtractionTechniquesof Sapindusmukurossi Saponineand DevelopmentofNewDetergent[J].Northern Horticulture,2013,37(07):143.
[9]金文斌,张凡兵.紫花苜蓿生长特性及品质对不同刈割强度的响应[J].北方园艺,2014,38(21):72.
JIN Wen-bin,ZHANG Fan-bing.Response of Quality and Growth Characteristics of Medicago sativa for the Different Intensity of Cutting[J].Northern Horticulture,2014,38(07):72.
[10]蒋向辉,苑静,祝军委,等.青钱柳采后加工及提取方法对青钱柳总皂苷含量的影响[J].北方园艺,2014,38(23):120.
JIANG Xiang-hui,YUAN Jing,ZHU Jun-wei,et al.Effect of Post-Harvest Processing and Extraction Methods on Total Saponin Content of Cyclocarya paliurus[J].Northern Horticulture,2014,38(07):120.
备注/Memo
第一作者简介：李小安（1978-），女，硕士，副教授，现主要从事高海拔地区牧草育种与生态环境保护等研究工作。E-mail：598581214@qq.com. 责任作者：杨国柱（1960-），男，教授，现主要从事高海拔地区牧草遗传育种与三江源植物资源开发利用等研究工作。E-mail：Yanggz110@126.com. 基金项目：青海省科学技术厅资助项目（2011-N-512）；华美源科研基金资助项目（SHJ-12-1）。