YANG Yali,LIU Jia,ZHANG Peng,et al.Effect of Different Concentrations of Colchicine on the Plyploid Breeding of Paris polyphylla[J].Northern Horticulture,2015,39(20):56-60.[doi:10.11937/bfyy.201520015]
秋水仙素对七叶一枝花种子多倍体诱导的影响
- Title:
- Effect of Different Concentrations of Colchicine on the Plyploid Breeding of Paris polyphylla
- Keywords:
- Paris polyphylla; colchicine; polyploid
- 文献标志码:
- A
- 摘要:
- 以七叶一枝花种子为试材，分别用浓度为0.025%、0.050%、0.100%、0.150%、0.200%的秋水仙素处理七叶一枝花种子12、24、48、60 h，研究不同浓度的秋水仙素及不同处理时间对七叶一枝花多倍体诱导的影响，以期为进一步选育七叶一枝花优良多倍体品种提供材料，为探索秋水仙素诱导七叶一枝花多倍体育种研究提供参考。结果表明：秋水仙处理的七叶一枝花根尖均有明显的膨大现象，其中浓度为0.050%秋水仙素处理12 h的根尖膨大率最高，为51.69%；秋水仙素处理的七叶一枝花种子的胚根短且粗、胚轴伸长；经秋水仙素处理种子萌发的组培苗植株较对照植株的根茎明显加粗、节间变短。采用流式细胞仪检测发现，秋水仙素处理样品的组培苗的幼根细胞DNA荧光强度约是对照细胞DNA荧光强度的1.88倍，表明经秋水仙素处理的七叶一枝花种子萌发的组培植株细胞染色体出现了部分加倍。
- Abstract:
- In order to investigate the influence of different concentrations of colchicine on the plyploid breeding of Paris polyphylla, P.polyphylla seeds as experimental materials were treated by colchicine with the concentration of 0.025%，0.050%，0.100%，0.150% and 0.200% for different time of 12，24，48，60 hours，respectively.The results showed that the root tip swelling of polyphylla seeds treated with different colchicine concentrations for different times were more than the control seeds untreated with colchicine，and the highest swelling rate was 51.69% under the treatment of 0.05% colchicine for 12 hours.The radicles of colchicine-treated P.polyphylla seeds and the plants originated from colchicine-treated seeds were shorter and stronger than the control untreated with colchicine.The detection results of flow cytometry showed that the DNA fluorescence intensity of the radicle cells of the plants originated from colchicine-treated seeds was about 1.88 folds of the control non-treated with colchicine，indicating that the chromosome of the plants originated from colchicine-treated seeds were partially doubled.These results provided references for the development of polyploid breeding of P.polyphylla.
参考文献/References:
[1]马林喜,王俊丽,费良丹,等.武陵山地区特色药用植物七叶一枝花的研究与应用[J].中央民族大学学报(自然科学版),2014,23(3):75-79. [2]左予桐.云南重楼抗肿瘤活性成分研究[D].天津:天津大学，2005. [3]何韩军,杨跃生,吴鸿.药用植物多倍体的诱导及生物学意义[J].中草药,2010,41(6):1000-1006. [4]杜艳伟,阎晓光,赵晋锋.药用植物多倍体育种的研究进展[J].生物技术进展,2011,1(4):249-253. [5]张爱民,常莉,薛建平.药用植物多倍体诱导研究进展[J].中国中药杂志,2005,30(9):645-649. [6]艾建国,高山林.丹参同源四倍体的诱导、鉴定及有效成分的含量测定[J].药物生物技术,2003,10(6):372-376. [7]武延生.秋水仙素诱导大蒜根尖多倍体的研究[J].北方园艺,2012(23):36-38. [8]张焕玲,李俊红,李周岐.秋水仙素处理杜仲种子诱导多倍体的研究[J].西北林学院学报，2008,23(1):78-81. [9]李桂双,刘强,于凤,等.秋水仙素诱导黄芩多倍体的初步研究[J].种子,2009,28(10):94-96. [10]张计育,李国平,乔玉山,等.秋水仙素对草莓离体叶片再生和多倍体诱导的影响[J].植物资源与环境学报，2009,18(3):69-73. [11]陈娟.西瓜四倍体人工诱变及无籽西瓜主要数量性状遗传研究[D].合肥:安徽农业大学,2006. [12]宋发军,黄宗华.七叶一枝花组织培养和种子萌发条件的研究[J].中南民族大学学报(自然科学版)，2013,32(2):51-54. [13]于文艳.大蒜四倍体植株诱导与鉴定[D].泰安:山东农业大学,2008. [14]韩莎,李四军,许晓东,等.利用流式细胞仪快速鉴定桑树细胞的染色体倍数性[J].蚕业科学,2013,39(6):1042-1048. [15]闫秋洁,杨琼.秋水仙素对蚕豆胚根生长的影响及多倍体诱导效应分析[J].广西植物，2012,32(5):386-391. [16]JIANG H E,LIU M J.Studies on polyploidy induction of Chinese jujube with colchicines[J].Acta Houtic Sin，2004,31(5):647-650. [17]CHEN X S,WEI L J,TIAN Y N，et al.Regeneration of polyploidy plants of cassava induced with colchincines[J].Chin J Trop Agric,2008,1:17-20.
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备注/Memo
第一作者简介：杨亚利（1986-），女，硕士研究生，研究方向为应用生物技术。E-mail：yangyaliwo@126.com.责任作者：张鹏（1977-），男，博士，副教授，现主要从事药用植物及其内生菌等研究工作。E-mail：zhangpenghust@126.com.基金项目：国家自然科学基金资助项目(31370118)；中南民族大学基本科研业务费专项资金资助项目（CZW15019）；校企合作资助项目（HZY14024）；中南民族大学大学生创新创业资助项目(GXX14205，GCX15006，SCX15004)。