LIU Xi-xing,GU Yu-hong,LI Bao-guo,et al.Study on Polyploid Induction Technique of Walnut[J].Northern Horticulture,2012,36(02):113-116.
核桃多倍体诱导技术研究
- Title:
- Study on Polyploid Induction Technique of Walnut
- 文章编号:
- 1001-0009（2012）02-0113-04
- Keywords:
- walnut; colchicine; polyploid induction; chromosome identification
- 分类号:
- S 6641036
- 文献标志码:
- A
- 摘要:
- 以‘绿岭’核桃种子、1 a生‘绿岭’核桃实生苗的新梢、5 a生采穗圃的‘绿岭’核桃新梢为试材，通过不同浓度的秋水仙素和不同时间的处理对核桃进行多倍体诱导，并用流式细胞仪进行倍性鉴定。结果表明：幼苗茎尖浸润法、新梢掐茎尖浸润法、种仁浸泡法、胚浸泡法、刺激隐芽法、刺激腋芽法处理后变异率均为0%，幼苗掐茎尖浸润法处理后，10%的秋水仙素溶液处理8 d的30株幼苗中有4株变异，变异率为133%。对叶片明显变大、变厚，叶色变深，叶片变皱的植株进行倍性鉴定表明，1株是四倍体，3株是混倍体。
- Abstract:
- The‘Lvling’ walnut seeds,the annual ‘Lvling’ walnut seedling shoots and 5 years old ‘Lvling’ walnut scion nursery shoots were used as materials to induct polyploid plants by different colchicine solution concentrations and different treated time,the materials were identified by flow cytometry.The results showed that the variation rates were 0% respectively with the methods of shoot apex infiltration,shoot top-pinching infiltration,seeds kernel immersion,embryo immersion,kryptoblast stimulation and timulating axillary bud stimulation;with the method of seedlings top-pinchinginfiltration，there were 4 varied plants in 30 seedlings after infiltrated with 10% colchicine solution for 8 d,and the variation rate was 133%.The plants whose leaves were notably larger,thicker,darker and wrinkled than that of normal were identified,the result showed that 1 plant was tetraploid,3 plants were mixoploidy.
参考文献/References:
[1]张毅萍.世界及我国核桃生产概况和几个问题[J].林业科技与市场信息,2002(3):52-55.
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备注/Memo
第一作者简介：刘喜星（1986-），女，河北保定人，在读硕士，现从事经济林栽培生理研究工作。E-mail:liuxixing1234@126.com。