GAO Yue,ZHU Xu-dong,SI Wen-hui,et al.Study on Influence and Effect of Microwave Sterilization on MS Medium[J].Northern Horticulture,2012,36(23):129-131.
微波灭菌对MS培养基的影响及效果
- Title:
- Study on Influence and Effect of Microwave Sterilization on MS Medium
- 文章编号:
- 1001-0009（2012）23-0129-03
- Keywords:
- MS medium; microwave sterilization; pH value; sterilization effect
- 分类号:
- Q 943.1
- 文献标志码:
- A
- 摘要:
- 分析测定了不同微波灭菌时间对MS培养基的温度、失水率、pH的影响并进行灭菌效果的比较，以期探讨MS培养基微波灭菌效果。结果表明：随着灭菌时间的增加，MS培养基的温度和失水率都随之上升，pH降低，灭菌时间达到2 min后，MS培养基彻底灭菌，无杂菌生长;且pH变化很小，营养成分破坏少。
- Abstract:
- The effect of different microwave sterilizations on the temperature，water loss rate，pH of MS medium were detected，and the sterilization effect was compared to investigate sterilization effect of MS medium.The results showed that with the increase of sterilization time，MS medium temperature and water loss rate rised，pH value reduced.When sterilization time reached 2 min，MS medium was thoroughly sterilization.There was no bacterial growth，and the pH value changed small，the nutrients of MS medium were damaged a little.
参考文献/References:
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备注/Memo
第一作者简介：高岳（1979-），男，江苏苏州人，硕士，讲师，研究方向为生物技术及农产品加工检测。