PU Shi-ming,LI Rong-feng,FANG Li-juan,et al.Tissue Culture and Clone Construction of Crassula argentea Thunb[J].Northern Horticulture,2012,36(23):125-128.
燕子掌的组织培养与植株再生
- Title:
- Tissue Culture and Clone Construction of Crassula argentea Thunb
- 文章编号:
- 1001-0009（2012）23-0125-04
- 分类号:
- S 681.903.6
- 文献标志码:
- A
- 摘要:
- 以燕子掌叶片为外植体，在培养基中添加不同浓度的6-BA和IBA，研究燕子掌组培苗形成过程中6-BA和IBA浓度对各阶段的影响。结果表明：MS+IBA 5 mg/L+6-BA 5 mg/L为愈伤组织诱导的最佳培养基，分化不明显，且诱导率为95.56％；MS+IBA 2 mg/L+6-BA 1 mg/L为外植体直接诱导不定芽的最佳培养基，平均可达12芽以上；MS+IBA 5 mg/L+6-BA 5 mg/L为愈伤组织增殖最佳培养基，增殖倍数为10.9；愈伤组织分化的最佳培养基为MS+IBA 1 mg/L+6-BA 5 mg/L，单位面积芽数为32个以上，且芽明显；壮苗的最佳培养基为MS+IBA 2 mg/L，45 d后平均苗长为4.74 cm；诱根的最佳培养基为MS+IBA 0.5 mg/L，诱根率为100％。
- Abstract:
- Taking Crassula argentea Thunb lamina as the explant and adding different concentrations of 6-BA and IBA in the culture medium，the influence of concentration of 6-BA and IBA on each stage in the Crassula argentea Thunb tissue culture forming process were studied.The results showed that MS+IBA 5 mg/L+6-BA 5 mg/L was the best medium for callus induction with unconspicuous differentiation and an inductivity of 95.56%；MS+IBA 2 mg/L+6-BA 1 mg/L was the best for adventitious bud induction directly with an inductivity of 86.67%，that could achieve more than 12 buds in average；MS+IBA 5 mg/L+6-BA 5 mg/L was the best for callus proliferation and the proliferation multiples was 10.9；the best for callus differentiation was MS+IBA 1 mg/L+6-BA 5 mg/L，the number of buds per unit area was more than 32 and the buds were obvious；when the medium was MS+IBA 2 mg/+6-BA 5 mg/L，the globular embryos produced more on the tissue surface with a long duration；the best for sound seeding was MS+IBA 2 mg/L，the average length of buds could be 4.74 cm after 45 d；on the medium with MS+IBA 0.5 mg/L，the inductivity of root inducing could be 100%.
参考文献/References:
[1]谢维荪.燕子掌与马齿觅树[J].中国盆景花卉,2006(5):56. [2]高世良.百花百话[M].天津:百花文艺出版社,2007:270-272. [3]周杰良,闫文德,王建湘.7种盆栽观赏植物室内滞尘能力研究[J].现代农业科技,2009(8):7-8. [4]周涤,王贤.居家装修必备解毒植物[M].北京:气象出版社,2007:78. [5]刘艳菊,葛红.室内观赏植物对苯和甲醛的净化研究及养护技术[M].北京:科学出版社,2010:68-69. [6]汪晓萍.观察叶片表皮的好材料-燕子掌[J].生物学通报,2004,39(7):23. [7]朱跃辰.用燕子掌叶制取不定根标本[J].生物学通报,1994,29(1):27. [8]王福东,汪涛,汪汉卿.燕子掌的驯化栽培[J].中国中药杂志,2007,23(4):341-342. [9]Liu Y L,Long Z L,Gao Y,et al.Organ formation and plant regenerationin vitrotissue culture of Crassula arborescens[J].Journal of Zhejiang University (Agric & Life Sci),2007,33(6):591-596. [10]刘建华,刘佳.观察探究玉树叶的无性繁殖[J].生物学通报,2010,45(8):58-59. [11]芦站根,周文杰.燕子掌不同发育时期内源激素含量变化研究[J].北方园艺,2008(12):123-126.
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备注/Memo
第一作者简介：蒲仕明（1987-），男，四川南充人，在读硕士，研究方向为细胞生物学和干细胞生物学。E-mail:pushiming77@163.com.