ZHANG Zheng-hai,ZHANG Yue,LI Ai-min,et al.Optimized Study on Growth of Tissue Culture Sprout of Schisandrachinensis(Turcz.)Baill[J].Northern Horticulture,2012,36(10):133-135.
五味子组培苗生长优化研究
- Title:
- Optimized Study on Growth of Tissue Culture Sprout of Schisandrachinensis(Turcz.)Baill
- 文章编号:
- 1001-0009（2012）10-0133-03
- 分类号:
- S 567.9
- 文献标志码:
- A
- 摘要:
- 以五味子新品种“红珍珠”无菌苗茎段为试材，以MS为基本培养基,采用L9（34）正交实验设计方法,研究了不同培养基与不同浓度NAA和6-BA组合对五味子组培苗初代接种和继代增殖的影响。结果表明：初代接种最适培养基为MS+0.1 mg/L 6-BA+0.01 mg/L NAA，继代增殖最佳培养基为MS+0.1 mg/L 6-BA+0.02 mg/L NAA，6-BA与NAA比值为5时，愈伤率高、愈伤组织大小适中，利于组培苗生长。
- Abstract:
- Chosen stem section of aseptic seedling of schisandrae new cultivar ‘Red pearl’ as test materials,and MS medium as minimal medium,effect of medium and different concentration NAA and 6-BA on first-generation inoculation and subculture of tissue culture sprout of Schisandrachinensis(Turcz.)Baill were studied by the methods of orthogonal test design f L9（34）.The results showed that the medium MS+0.1 mg/L 6-BA+0.01 mg/L NAA was suitable for the early generation inoculation,the medium MS+0.1 mg/L 6-BA+0.02 mg/L NAA was suitable for subculture and growth.When the ratio of 6-BA/NAA was 5，the rate of callus and the size of callus was moderate and was conducive to tissue culture sprout growth.
参考文献/References:
[1]江苏新医学院.中药大词典[M].上海:上海科学技术出版社,2009:387-388.
[2]李爱民.北五味子栽培与选种技术[M].北京:金盾出版社,2008:12-24.
[3]刘丽娟.激素对五味子愈伤组织诱导的影响[J].通化师范学院学报,2007,28(4):20-21.
[4]武立丹,金东淳,周桐,等.北五味子愈伤组织诱导研究[J].延边大学农学学报,2008，30(3):167-171.
[5]朱俊义,刘雪莲,刘立娟,等.通化师范学院生物系[J].植物生理学通讯,2006,42(3):580.
[6]牛遇达.北五味子体细胞胚胎发生[D].哈尔滨:东北林业大学,2007.
[7]张晓薇,夏媛,李凤兰,等.北五味子组织培养研究[J].安徽农业科学,2009,37(9):3933-3935.
[8]张佳,金东淳,金海林,等.筛选北五味子愈伤组织诱导中不同因素的最佳条件[J].延边大学农学学报,2011,33(1):40-44.
[9]陈丽静,齐欣,王玉坤,等.北五味子快繁体系的建立[J].中草药,2011,42(3):575-578.
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备注/Memo
第一作者简介：张正海（1981-），男，硕士，研究实习员，现主要从事药用植物栽培及育种等研究工作。责任作者:李爱民（1956-），男，硕士，研究员，现主要从事药用植物栽培及育种等研究工作。
基金项目：吉林省科技发展计划资助项目（20080904）。
收稿日期：2012-03-05