SUN Xuewen,SHENG Yunyan,JIN Yazhong,et al.Isolation and Identification of Bacterilal Fruit Blotch and Screening of Resistant Germplasm Resources[J].Northern Horticulture,2024,(6):25-32.[doi:10.11937/bfyy.20233577]
甜瓜细菌性果斑病病原菌的分离鉴定与抗性种质资源的筛选
- Title:
- Isolation and Identification of Bacterilal Fruit Blotch and Screening of Resistant Germplasm Resources
- 文章编号:
- 1001-0009(2024)06-0025-08
- 分类号:
- S 652
- 文献标志码:
- A
- 摘要:
- 以黑龙江省大庆市大同区采集的甜瓜细菌性果斑病病叶及研究室保存的22份甜瓜材料为试材，采用病原菌的分离纯化、形态学鉴定、致病性鉴定、分子鉴定及抗病性鉴定等方法，研究了大同区引起薄皮甜瓜细菌性果斑病的主要病原菌菌株类群及甜瓜材料对该菌株的抗病表现，以期为甜瓜细菌性果斑病的防治提供参考依据。结果表明：通过分离、纯化从发病植株叶片上获得了1株细菌性果斑病病原菌，命名为HLJ23；该菌株接种到健康叶片后发病症状与甜瓜细菌性果斑病的症状相似；进一步通过分子鉴定确定该菌株为燕麦嗜酸菌西瓜亚种(Acidovorax citrulli)亚群I型菌株；研究室保存的22份甜瓜材料中感病材料多于抗病材料，其中8份表现抗病，14份表现感病。综上所述，‘1185’和‘258’是黑龙江省大庆市大同区薄皮甜瓜细菌性果斑病病原菌的优势种群。
- Abstract:
- Taking muskmelon bacterial fruit spot disease leaves collected from Datong district，Daqing city，Heilongjiang Province and 22 muskmelon materials preserved in the laboratory as test materials，the main pathogenic bacterial strains causing bacterial fruit spot disease in thin-skinned muskmelon in Datong District were studied by the methods of isolation and purification，morphological identification，pathogenicity identification，molecular identification，and disease resistance identification of pathogenic bacteria.The resistance performance of muskmelon materials preserved in the laboratory to this strain was also studied，in order to provide reference for the prevention and control of bacterial fruit spot disease in muskmelon.The results showed that one bacterial fruit spot pathogen was obtained from the leaves of diseased plants by isolation and purification，named HLJ23.The pathogenesis of this strain after inoculation into healthy leaves was similar to that of BFB.Further molecular identification was determined to be a subgroup I strain of Acidovorax citrulli.Among the 22 melon materials preserved in the laboratory，there were more susceptible materials than disease-resistant materials，of which 8 were resistant and 14 were susceptible.In summary，‘1185’ and ‘258’ were the dominant population of bacterial fruit spot pathogen in thin-skinned muskmelon in Datong District，Daqing City，Heilongjiang Province.
参考文献/References:
［1］马海州,吕鹏程,杨爱华,等.保护地西瓜细菌性果斑病发生规律与防治技术研究［J］.农民致富之友,2017(2):61.［2］毛云,苏小龙,宁雪飞,等.甜瓜果斑病致病基因生物信息学分析［J］.北方园艺,2017(2):105-111.［3］张兴平,BILLY B R.一种毁灭性的西瓜新病害:细菌性果实腐斑病［J］.中国西瓜甜瓜,1990,3(2):44-45.［4］YAN L,HU B,CHEN G,et al.Further evidence of cucurbit host specificity among Acidovorax citrulli groups based on a detached melon fruit pathogenicity assay［J］.Phytopathology,2017,107(11):1305-1311.［5］ZHONG J,LIN Z Y,MA Y M,et al.Rapid discrimination between groups Ⅰ and Ⅱ of Acidovorax citrulli using a primer pair specific to a pilL gene［J］.Journal of Phytopathology,2016,164(7/8):558-562.［6］YAN S,YANG Y,WANG T,et al.Genetic diversity analysis of Acidovorax citrulli in China［J］.European Journal of Plant Pathology,2013,136(1):171-181.［7］SONG J Y,PARK S Y,SEO M W,et al.Genetic characteristics of Acidovorax citrulli population causing bacterial fruit blotch against cucurbits in Korea［J］.Research in Plant Disease,2015,21(2):82-88.［8］刘梦华,卢霞,李腾,等.甜瓜细菌性果斑病病菌鉴定及不同材料抗病性测定［J］.安徽农业科学,2021,49(6):145-148.［9］罗群,仇智灵,孙超,等.瓠瓜栽培品种细菌性果斑病的抗性鉴定［J］.浙江农业科学,2021,62(6):1170-1172,1196.［10］韩宏伟,刘会芳,韩盛,等.哈密瓜种质资源苗期细菌性果斑病抗性鉴定［J］.新疆农业科学,2018,55(10):1863-1869.［11］张亮,王惠林,万秀琴,等.不同甜瓜材料苗期细菌性果斑病抗性鉴定［J］.北方园艺,2016(9):117-120.［12］刘凌云,严斌,李文才,等.哈密瓜细菌性果斑病及其防治措施［J］.现代农业,2008(9):23-24.［13］李菊芬,林涛,马国斌.甜瓜细菌性果斑病带菌种子的处理方法研究［J］.上海农业学报,2020,36(6):44-48.［14］方中达.植病研究方法［M］.3版.北京:中国农业出版社,1998.［15］BAHAR O,EFRAT M,HADAR E,et al.New subspecies-specific polymerase chain reaction-based assay for the detection of Acidovorax avenae subsp.citrulli［J］.Plant Pathology,2008,57(4):754-763.［16］YANG Y W,ZHAO M,ZHANG L Q,et al.Development of a multiplex PCR assay based on the pilA gene sequences to detect different types of Acidovorax citrulli［J］.Journal of Microbiological Methods,2019,158:93-98.［17］REN Y Z,LI H,LI G Y,et al.First report of Acidovorax avenae subsp.citrulli infecting edible seed watermelon (Citrullus lanatus var.lanatus) in China［J］.Plant Disease,2006,90(8):1112.［18］贾宋楠,王惠林,郑健,等.籽用西瓜种质资源对细菌性果斑病的抗性鉴定［J］.新疆农业科学,2013,50(5):864-869.［19］宁雪飞.甜瓜果斑病抗性鉴定及抗果斑病和霜霉病QTLs定位［D］.乌鲁木齐:新疆大学,2018.［20］杨志毅,王瑞,王超楠,等.甜瓜不同时期铜敏感性［J］.北方园艺，2022(16):39-45.［21］蔡馥宇,关巍,乔培,等.瓜类细菌性果斑病研究新进展［J］.中国瓜菜,2017,30(11):1-5.［22］刘丹丹,刘畅.甜瓜细菌性果斑病菌鉴定及杀菌剂毒力测定［J］.北方园艺,2017(18):6-10.［23］朱晓媛,王文成,徐烨,等.云南省瓜类细菌性果斑病的鉴定［J］.云南农业大学学报(自然科学),2013,28(2):175-179.［24］武芳.瓜类细菌性果斑病的生物防治研究［D］.南京:南京农业大学,2013.［25］于海博.瓜类细菌性果斑病的病原鉴定、快速检测及防控技术研究［D］.沈阳:沈阳农业大学,2019.［26］于畅,吴林娜,赵立萍,等.西瓜噬酸菌Ⅲ型分泌系统hrcQ基因功能分析［J］.植物保护,2019,45(2):48-56.［27］李俊阁,王惠林,张亮,等.不同甜瓜材料苗期对细菌性果斑病抗病性鉴定［J］.新疆农业科学,2015,52(10):1843-1848.
相似文献/References:
[1]王晓静,马文平,王芳.甜瓜采后致病菌交链菌的防治方法研究[J].北方园艺,2014,38(04):103.
WANG Xiao-jing,MA Wen-ping,WANG Fang.Study on Prevention Method of Alternariasp. After Melon Postharvest[J].Northern Horticulture,2014,38(6):103.
[2]宁雪飞,韩小路,王贤磊,等.一种快速筛选转抗霜霉病基因甜瓜种子的方法研究[J].北方园艺,2014,38(01):102.
NING Xue-fei,HAN Xiao-lu,WANG Xian-lei,et al.The Screening of Transgenic Plants Resistant to Downy Mildew Disease in Melon[J].Northern Horticulture,2014,38(6):102.
[3]王晓梅,刘国宁,程丽,等.部分黄瓜和甜瓜品种对霜霉病的抗性及其组分研究[J].北方园艺,2014,38(04):106.
WANG Xiao-mei,LIU Guo-ning,CHENG Li,et al.Study on Resistance to Downy Mildew of Some Cucumber and Melon Varieties and Its Component[J].Northern Horticulture,2014,38(6):106.
[4]张君艳,罗爱玉,张君君.河西地区杂交甜瓜大田制种生产技术[J].北方园艺,2013,37(14):62.
[J].Northern Horticulture,2013,37(6):62.
[5]苏艳,王盼乔,胡建斌.十五份甜瓜种质苗期性状的聚类分析及相关分析[J].北方园艺,2014,38(07):5.
SU Yan,WANG Pan-qiao,HU Jian-bin.Clustering and Correlation Analysis of 15 Cucumis melo L.Germplasms Based on the Phenotypic Traits of the Seedling Period[J].Northern Horticulture,2014,38(6):5.
[6]赵九洲,胡立盼,徐志然,等.甜瓜幼苗耐盐碱性及缓解盐碱胁迫γ-氨基丁酸浓度的筛选[J].北方园艺,2014,38(09):1.
ZHAO Jiu-zhou,HU Li-pan,XU Zhi-ran,et al.The Screening of Melon（Cucumis melo L.）Seedling Cultivars for Salt-alkaline Tolerance and the Concentration of γ-aminobutyric Acid Alleviating Salt-alkaline Stress[J].Northern Horticulture,2014,38(6):1.
[7]王喜涛,周秀艳,辛 明,等.盐胁迫对甜瓜种子发芽的影响[J].北方园艺,2014,38(09):7.
WANG Xi-tao,ZHOU Xiu-yan,XIN Ming,et al.Effect of NaCl Stress on Seed Germination of Melon[J].Northern Horticulture,2014,38(6):7.
[8]宋 羽,卢青成,王 浩.适合新疆戈壁地区甜瓜穴盘育苗的基质相对含水量的探究[J].北方园艺,2014,38(09):53.
SONG Yu,LU Qing-cheng,WANG Hao.Research on Suitable Substrate Water Content for Melon Plug-seedling in Gobi District of Xinjiang[J].Northern Horticulture,2014,38(6):53.
[9]苏丽影,宋述尧,赵春波,等.玉米秸秆混合基质对甜瓜穴盘苗生长的影响[J].北方园艺,2013,37(12):28.
SU Li-ying,SONG Shu-yao,ZHAO Chun-bo,et al.Effect of Mixed Substrates of Corn Stalk on Plug Seeding of Muskmelon[J].Northern Horticulture,2013,37(6):28.
[10]王爱玲,张敏,廖新福,等.甜瓜“黄醉仙”下胚轴再生体系的初步建立[J].北方园艺,2013,37(12):100.
WANG Ai-ling,ZHANG Min,LIAO Xin-fu,et al.Preliminary Establishment of Melon cv.‘Huangzuixian’ Regeneration System[J].Northern Horticulture,2013,37(6):100.
[11]张亮,王惠林,万秀琴,等.不同甜瓜材料苗期细菌性果斑病抗性鉴定[J].北方园艺,2016,40(09):117.[doi:10.11937/bfyy.201609031]
ZHANG Liang,WANG Huilin,WAN Xiuqin,et al.Identification of Resistance to Acidovorax citrulli of Different Melons in the Seedling Stage[J].Northern Horticulture,2016,40(6):117.[doi:10.11937/bfyy.201609031]
[12]万秀琴,王惠林.不同药剂对瓜类幼苗细菌性果斑病防治效果比较[J].北方园艺,2017,41(12):129.[doi:10.11937/bfyy.201712029]
WAN Xiuqin,WANG Huilin.Control Efficacy of Different Chemical on Bacterial Fruit Blotch of Cucurbits Seedling[J].Northern Horticulture,2017,41(6):129.[doi:10.11937/bfyy.201712029]
备注/Memo
第一作者简介：孙雪雯（2000-），女，硕士研究生，研究方向为瓜类作物抗病育种。E-mail：sunxuewen2207@163.com.责任作者：盛云燕（1979-），女，博士，教授，现主要从事瓜类作物抗病育种等研究工作。E-mail：shengyunyan@byau.edu.cn.基金项目：大庆市指导性科技计划资助项目（zd-2023-62）；黑龙江省自然科学基金联合资助项目（LH2022C065）；黑龙江八一农垦大学博士启动资助项目（XYB202116）。收稿日期：2023-10-19