WANG Ruixue,GUO Jie,HAO Nan,et al.Identification and Analysis of 14-3-3 Gene Family in Daucus carota[J].Northern Horticulture,2024,(6):17-24.[doi:10.11937/bfyy.20233424]
胡萝卜14-3-3基因家族的鉴定与分析
- Title:
- Identification and Analysis of 14-3-3 Gene Family in Daucus carota
- 文章编号:
- 1001-0009(2024)06-0017-08
- 关键词:
- 胡萝卜; 14-3-3基因家族; 系统进化分析
- Keywords:
- carrot; 14-3-3 gene family; phylogeny analysis
- 分类号:
- S 631.2
- 文献标志码:
- A
- 摘要:
- 以胡萝卜为试材,采用分子生物学和生物信息学方法对胡萝卜14-3-3基因家族各成员进行鉴定,研究分析其理化特性、基因结构、保守结构域、复制事件、系统进化及顺式作用元件,以期为进一步研究胡萝卜14-3-3蛋白的功能提供参考依据。结果表明:胡萝卜中共鉴定到11个14-3-3基因,不均匀分布在6条染色体上,含有3~7个外显子,其中1对基因发生片段复制事件;编码的蛋白序列长度为236~264 aa,分子量为26.64~30.14 kD,等电点为4.65~5.33,具有7个Motif,全部为酸性非分泌型蛋白,定位在细胞核。系统进化分析显示,胡萝卜14-3-3蛋白分为ε类(3个)与非ε(8个)类2个亚族。此外,顺式作用元件分析结果表明,胡萝卜14-3-3基因含有大量激素与逆境胁迫相关元件。
- Abstract:
- Taking carrot as the test material,14-3-3 gene family in carrot were identified and their physicochemical properties,gene structure,conserved domains,replication events,phylogenetic evolution and cis-acting elements were analyzed,in order to provide reference for further understanding the function of 14-3-3 proteins in carrot.The results showed that a total of 11 14-3-3 genes were identified in carrot,unevenly distributed on six chromosomes,containing 3 to 7 exons,and one pair of 14-3-3 genes underwent segmental duplication.Carrot 14-3-3 proteins contained 236-264 amino acid residues,with molecular weight of 26.64-30.14 kD,isoelectric point of 5.84-9.14,and 7 conserved motifs.All 14-3-3 proteins of carrot were acidic and non-secreted proteins and located in the nucleus.Phylogenetic analysis indicated that the 14-3-3 proteins of carrot were divided into ε-type (3 members) and non ε-type (8 members).In addition,cis-acting element analysis showed that carrot 14-3-3 genes contained a large number of hormone-and stress-related elements.
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备注/Memo
第一作者简介:王瑞雪(1985-),女,硕士,讲师,现主要从事植物系统与进化等研究工作。E-mail:369906560@qq.com.基金项目:甘肃省高等学校创新基金资助项目(2022B-437)。收稿日期:2023-10-09