PENG Hao,CAO Dan,SI Doudou,et al.Analysis of Genetic Diversity and Germination Promoting Effect of Gastrodia elata Germinating Fungi in Hanzhong[J].Northern Horticulture,2024,(2):118-124.[doi:10.11937/bfyy.20232599]
汉中地区天麻萌发菌遗传多样性和促萌效果分析
- Title:
- Analysis of Genetic Diversity and Germination Promoting Effect of Gastrodia elata Germinating Fungi in Hanzhong
- 文章编号:
- 1001-0009(2024)02-0118-07
- Keywords:
- Gastrodia elata; germination fungi; antagonism; ITS; esterase isozyme
- 分类号:
- Q 939
- 文献标志码:
- A
- 摘要:
- 以汉中地区9株天麻萌发菌及天麻花粉种子为试材，采用拮抗试验、ITS序列测定、酯酶同工酶技术和种子拌播等方法，研究了9株天麻萌发菌的遗传多样性、亲缘关系和促萌效果，以期为天麻萌发菌的种质资源管理与评价等提供参考依据。结果表明：9株萌发菌根据两两拮抗试验的程度不同分为不显著、显著和极显著3类；根据ITS序列的差异分为Mycena sp.和Heydenia sp. 2类；根据酯酶同工酶酶谱条带在80% 的相似水平上可分为5类；9株萌发菌与天麻种子共生萌发，其中C3菌株菌丝生长速度最高为（0.30±0.01）cm·d-1，C2菌株的萌发率最高为64.66%±2.89%，C2和C3菌株的综合表现最佳。
- Abstract:
- The genetic diversity，genetic relationship and germination promoting effect of 9 Gastrodia elata germinating fungi and their pollen seeds in Hanzhong area were studied by means of antagonistic experiment，ITS sequence determination，esterase isozyme technology and seed mixing，in roder to provide reference for the management and evaluation of germplasm resources of Gastrodia elata germinating fungi.The results showed that 9 germination fungi were classified into 3 types，insignificant，significant and extremely significant according to the degree of antagonism of each pair；according to the difference of ITS sequence，they were classified into Mycena sp.and Heydenia sp.2；according to esterase isozyme.The zymogram bands could be divided into 5 categories at a similar level of 80%；9 germination strains co-germinate with Gastrodia elata seeds，of which the highest growth rate of C3 strain was (0.30±0.01)cm·d-1，and the highest germination rate of C2 strain was 64.66%±2.89%，the comprehensive performance of C2 and C3 strains were the best.
参考文献/References:
［1］王彩云,侯俊,王永,等.天麻种子萌发菌研究进展［J］.北方园艺,2017(12):198-202.［2］谢海彬,叶彦慧,解修超,等.蜜环菌新品种“陕蜜2009”的选育［J］.北方园艺,2022(5):155-160.［3］秦丽媛.天麻萌发菌小菇生长条件及其多样性研究［D］.昆明:云南大学,2018.［4］陈艳红.北京地区兰科菌根真菌资源的发掘和利用［D］.北京:北京协和医学院,2018.［5］封海东,金善忠,周明,等.十堰市杂交天麻零代种高效制作与培育技术初探［J］.湖北农业科学,2019(11):83-86.［6］游中华.天麻萌发菌种制作和应用技术的改进［J］.湖北林业科技,2016(5):84-85.［7］程立君,李世辉,胡志芳,等.一株乌天麻共生萌发菌培养条件的优化［J］.北方园艺,2016(19):175-177.［8］谢学强.天麻栽培中萌发菌及蜜环菌的简易培养技术（英文）［J］.Agricultural Science & Technology,2016(9):2005-2007,2016.［9］虞小燕,邓百万,陈文强,等.基于形态学特征和ITS序列分析秦巴山区天麻萌发菌的亲缘关系［J］.江苏农业科学,2016(5):245-248.［10］郑朋朋,李珊,张保,等.苯酚硫酸法测定萌发菌HL-003多糖方法的研究［J］.食品工业科技,2016(6):74-77,85.［11］EUNG-JUN PARK,WI YOUNG LEE.In vitro symbiotic germination of myco-heterotrophic Gastrodia elata by Mycena species［J］.Plant Biotechnology Reports,2013(7):185-191.［12］刘锦波,杨杰,郝建玺,等.天麻种子共生菌:萌发菌生产的关键技术［J］.中国农业信息,2016(7):105-107.［13］虞小燕.天麻萌发菌遗传多样性的研究及优良菌株筛选［D］.汉中:陕西理工学院,2016.［14］薛婉秋.乌天麻有性繁殖研究［D］.重庆:重庆师范大学,2016.［15］王云生.黔东南州天麻产业发展现状、存在问题及对策［J］.贵州农业科学,2016(2):125-128.［16］李振斌,黄再强,胡明勋,等.天麻种子成熟度对其萌发、生长的影响研究［J］.中药与临床,2016(1):11-14.［17］刘爱萍,薛峰,陈建军,等.长白山乌秆天麻种子萌发菌的分离及选育［J］.吉林林业科技,2015(6):7-8,56.［18］郑朋朋,杨晓波,李珊,等.萌发菌HL-003胞内多糖的提取及抗氧化性研究［J］.中国酿造,2015(3):71-75.［19］祝友春,桑子阳,杨武松,等.天麻的有性繁殖栽培技术［J］.湖北林业科技,2015(4):77,90.［20］冉砚珠,徐锦堂,路淑芳.紫萁小菇等天麻种子萌发菌生物学特性及种子共生萌发条件的研究［J］.中草药,1990(9):29-32,47.［21］黄晨阳,张金霞,陈强,等.食用菌菌种真实性鉴定酯酶同工酶电泳法，NY/T 1097-2006［S］.北京:中华人民共和国农业部.［22］郭顺星,徐锦堂.促进天麻等兰科药用植物种子萌发的真菌酯酶同工酶研究［J］.中国药学杂志,1991(9):524-526,569.［23］TAYOR D L,MCCORMICK M K.Internal transcribed spacer primers and sequences for improved characterization of basidiomycetous orchid mycorrhizas［J］.New Phytol,2008,177:1120-1033.［24］谢果珍,申爱荣,谭著明,等.天麻共生菌研究进展［J］.湖南中医杂志,2015(4):206-208.［25］高燕燕,杭烨,刘剑东,等.兰科药用植物共生真菌促进种子萌发研究进展［J］.植物生理学报,2020,56(2):141-150.［26］贾少杰，解修超,等.基于酯酶同工酶和ITS序列对陕南栽培灵芝亲缘关系的研究［J］.河南农业科学,2018,47(8):95-101.
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备注/Memo
第一作者简介：彭浩（1979-），男，硕士，高级实验师，硕士生导师，现主要从事微生物资源的保护与利用等研究工作。E-mail：huaohao808@126.com.基金项目：陕西省重点研发计划资助项目(2023XCZX2-87)；陕西省大学生创新创业资助项目(S202110720104)。收稿日期：2023-08-01