WANG Ying,DU Pengfei,LI Juan.Effects of Different Microbial Fertilizers on Photosynthetic Physiology，Flavonoids Content and Soil Microbial Quantity of Glycyrrhiza uralensis[J].Northern Horticulture,2023,(18):103-110.[doi:10.11937/bfyy.20225277]
不同微生物菌肥对甘草光合生理、黄酮含量及土壤微生物的影响
- Title:
- Effects of Different Microbial Fertilizers on Photosynthetic Physiology，Flavonoids Content and Soil Microbial Quantity of Glycyrrhiza uralensis
- 文章编号:
- 1001-0009(2023)18-0103-08
- Keywords:
- microbial fertilizer; Glycyrrhiza uralensis; chlorophyll fluorescence characteristics; flavonoids; soil microorganisms
- 分类号:
- S 567.71
- 文献标志码:
- A
- 摘要:
- 以甘草(Glycyrrhiza uralensis)为试材，采用大棚划区试验，设置施用淡紫紫孢菌(Purpureocillium lilacinum)菌肥(PL)、枯草芽孢杆菌(Bacillus subtilis)菌肥(BS)、哈茨木霉真菌(Trichoderma harzianum)菌肥(TH)及其三者复合菌肥处理(MX)，以不施用菌肥为对照(CK)，探索了不同微生物菌肥对甘草光合色素、叶绿素荧光、PSⅡ光化学特征、黄酮组分及土壤微生物数量的影响，以期为微生物菌肥应用于甘草生产提供参考依据。结果表明：与CK相比，微生物菌肥处理(PL、BS、TH、MX)均在一定程度上提高了光合色素(叶绿素a、叶绿素b、类胡萝卜素)含量、增强了叶绿素荧光(Fv/Fm、qP、ΦPSⅡ)，降低了PSⅡ光化学损耗特征(φDo、φPo、Ro、Ψo)；同时提高了黄酮组分(甘草素、异黄酮、二氢黄酮、查尔酮)含量，各处理整体表现为CK＜PL、TH＜MX、BS。此外，微生物菌肥亦提高了土壤细菌、氨氧化细菌、放线菌及固氮菌数量，而对真菌无明显影响，以MX、BS处理较优。综上，施用微生物菌肥可提高光合作用效率和土壤微生物数量，同时提高黄酮类物质含量，以施用枯草芽孢杆菌(Bacillus subtilis)菌肥最佳。
- Abstract:
- Taking Glycyrrhiza uralensis as the test material，the green house paitition test was curried ont.Set up no application microbial fertilizer as control treatment (CK)，and applying Purpureocillium lilacinum (PL)，Bacillus subtilis (BS)，Trichoderma harzianum (TH) and tis compound treatment (MX)，which carried out to explore the effects of different microbial fertilizers on photosynthetic pigments，chlorophyll fluorescence，PSⅡ photochemical characteristics，flavonoid synthesis and soil microbial quantity of Glycyrrhiza uralensis,in order to provide reference for microbial fertilizer of Glycyrrhiza uralensis.The results showed that compared with CK treatment，microbial fertilizer treatments (PL，BS，TH，MX) increased the content of photosynthetic pigments (chlorophyll a，chlorophyll b，carotenoids)，enhanced chlorophyll fluorescence (Fv/Fm，qP，ΦPSⅡ)，and reduced the characteristics of PSⅡ photochemical loss characteristics (φDo，φPo，Ro，Ψo)，the flavonoid components (glycyrrhizin，glycyrrhisoflavone，licorice dihydroflavone，licorice chalcone) were increased，and the overall performance of each treatment was CK
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备注/Memo
第一作者简介：王莹（1979-），女，硕士，讲师，现主要从事微生物检验与教学等研究工作。E-mail:294464337@qq.com.责任作者：杜鹏飞（1986-），男，硕士，讲师，现主要从事重要药药学与有机合成等研究工作。E-mail:Dupf86@126.com.基金项目：国家自然科学基金面上资助项目（81373910）。收稿日期：2022-12-30