ZHANG Caini,DENG Baiwan,BAI Qiuyue,et al.Comparative Analysis of Extracellular Polysaccharide and Bioactivity Associated of Lentinus edodes[J].Northern Horticulture,2021,(20):126-131.[doi:10.11937/bfyy.20211402]
香菇胞外多糖及生物活性的对比
- Title:
- Comparative Analysis of Extracellular Polysaccharide and Bioactivity Associated of Lentinus edodes
- 文献标志码:
- A
- 摘要:
- 以L135、香66、808、日大4株香菇菌株为试材，采用苯酚-硫酸法、滤纸片扩散法及分光光度计法，研究了香菇胞外多糖含量、抑菌活性和抗氧化活性的差异，以期为香菇药食资源开发提供参考依据。结果表明：L135、808、香66、日大4株香菇的胞外多糖含量分别为（2.82±0.02）（1.61±0.04）（1.85±0.03）（2.22±0.06）mg?mL-1。当多糖质量浓度达到1.0 mg?mL-1时，菌株香66的还原能力最强，菌株日大的ABTS+自由基清除率最高，其清除率为39.97%；菌株L135的DPPH自由基清除率最高，其EC50值为3.53 mg?mL-1；菌株808的对羟自由基清除能力和抑菌活性最强。
- Abstract:
- L135,Xiang 66,808,Rida four strains of Lentinus edodes were used as the research objects.The contents of extracellular polysaccharides were determined by phenol-concentrated sulfuric acid method,the inhibition of bacteria was studied by filter paper diffusion method,and the antioxidant activity of polysaccharides in vitro was investigated by spectrophotometer,in order to provide reference for the development of Lentinus edodes medicinal and food resources.The results showed that the extracellular polysaccharide contents of Lentinus edodes L135,Xiang 66,808 and Rida were (2.82±0.02)mg?mL-1,(1.85±0.03)mg?mL-1,(1.61±0.04)mg?mL-1 and (2.22±0.06)mg?mL-1.When the concentration of Xiang 66 reached 1.0 mg?mL-1,the reduction ability of strain Xiang 66 were better than those of other three strains,the scavenging rate of strain Rida to ABTS+ radicals was 39.79%,higher than that of the other three strains,DPPH? scavenging rate of strain L135 were better than those of the other three strains,with EC50 of 3.53 mg?mL-1,respectively,the scavenging rates of hydroxyl free radical by 808 were higher than the others,and the antimicrobial activity of exopolysaccharide from strain 808 was higher than that of the other three agaricus blazei strains.
参考文献/References:
[1]YANG M D,LONG Z F,LI J.Study on the flavor components of Lentinus edodes[J].Food Science,2006(5):223-226.[2]侯爱萍,张树梅.香菇多糖抗菌抗病毒普适性研究[J].药学研究,2015,34(4):199-201.[3]翟飞红,张勇,赵文婧,等.羊肚菌、香菇、真姬菇子实体体外抗氧化活性的比较[J].食用菌,2020,42(4):75-79.[4]雨田,练心洁.对药用真菌多糖抗肿瘤作用机制的研究进展[J].当代医药论从,2020,18(3):27-29.[5]张晶,苏畅,贾英杰,等.香菇多糖抗肿瘤治疗临床应用进展[J].天津中医药,2019,36(11):1137-1140.[6]陈慧婵.香菇(Lentinus edodes)对小鼠抗疲劳、耐缺氧及抗氧化作用的研究[D].南京:南京农业大学,2014.[7]曾琳娜.香菇多糖降脂作用的评估及分子机制的研究[D].长沙:中南林业科技大学,2016.[8]于丽红.香菇多糖对H2O2诱导的SH-SY5Y细胞损伤的保护作用[D].济南:山东大学,2011.[9]杜景东,田凤娟,陈培雄,等.云雾绿茶多糖提取方法的优化及Sevage法除蛋白的工艺研究[J].中南药学,2015,13(12):1281-1283.[10]王文丽.15种中药材表面污染真菌的分离鉴定及产毒性能分析[D].广州:广州中医药大学,2013.[11]王祥红,汤志宏,李静.曲霉菌的分离及其形态观察[J].生物学通报,2013,48(7):54-55,63.[12]任朝辉,冯国辉,张传博.姬松茸深层发酵多糖提取工艺优化及抗氧化活性[J].食品工业科技,2017,38(14):216-222.[13]李福后,王伟霞,刘伟.影响金针菇菌丝体及胞外多糖产量的因子研究[J].食品研究与开发,2018,39(11):135-138.[14]解修超,刘军生,罗阳兰,等.4株姬松茸胞外多糖含量和生物活性的对比分析[J].食品研究与开发,2019,40(12):68-75.[15]罗阳兰,邓百万,刘军生,等.白及花与块茎石油醚部位成分分析及其生物活性的比较研究[J].中国现代应用药学,2019,36(4):444-450.[16]刘军生,解修超,罗阳兰,等.Aspergillus sp.EA-LJS80的分离及其发酵液提取物七叶皂苷C的生物活性[J].菌物学报,2018,37(1):79-87.[17]吴永祥,王祥,江海涛,等.不同极性柳叶蜡梅叶萃取物总酚含量及其抗氧化抑菌能力研究[J].食品与机械,2017,33(8):150-154.[18]柏秋月,邓百万,杨学英,等.4株羊肚菌胞外多糖含量及其生物活性的研究[J].食品研究与开发,2020,41(8):25-31.[19]杨少辉,宋英今,王洁华,等.雪莲果体外抗氧化和自由基清除能力[J].食品科学,2010,31(17):166-169.[20]赵秀玲,刘永杰.地皮菜挥发油的化学成分及抗氧化性研究[J].云南大学学报(自然科学版),2017,39(3):454-462.[21]熊利芝,付柏婷,吴玉先,等.栝楼籽多糖提取工艺优化及抗氧化性[J].应用化工,2015,44(6):1008-1011.[22]WANG J J,MEMG X J.Effect of purification on antibacterial activity of Acanthopanax sessiliflorus var.parviceps polysaccharide[J].Science and Technology of Food,2011,32(12):173-174.[23]钟耀广,林楠,王淑琴,等.香菇多糖的抗氧化性能与抑菌作用研究[J].食品科技,2007(7):141-144.[24]施洪飞,项平,杨立坤,等.泽泻香菇调节血脂和抗氧化作用实验研究[J].南京中医药大学学报(自然科学版),2000(2):93-95.[25]邵佳甲,梁英武,王和元,等.香菇和金针菇提取物抗氧化能力的比较研究[J].农业与技术,2016,36(19):42-43.
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备注/Memo
第一作者简介：〖JP3〗张彩妮（1994-），女，硕士研究生，研究方向为微生物资源保育与开发利用。E-mail:984365864@qq.com.责任作者：邓百万（1963-），男，硕士，教授，现主要从事微生物资源保育与开发利用等研究工作。E-mail:2210309868@qq.com.基金项目：陕西省重点研发计划资助项目（2017ZDXM-NY-001）。收稿日期：2021-03-31