LU Hong,LI Ming,LI Longming.Allelotoxic Effect of Aqueous Extract Solution From Pogostemon cablin[J].Northern Horticulture,2021,(16):108-115.[doi:10.11937/bfyy.20210042]
广藿香植株水提液的化感自毒作用
- Title:
- Allelotoxic Effect of Aqueous Extract Solution From Pogostemon cablin
- Keywords:
- Pogostemon cablin; water extract liguid; phenolic acids; allelotoxic substances; continuous cropping obstacle
- 文献标志码:
- A
- 摘要:
- 以广藿香组培驯化苗为试材，采用盆栽试验，研究了广藿香根、茎、叶水提液对广藿香幼苗根系活力、总叶绿素含量、丙二醛（MDA）含量、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性和超氧化物歧化酶（SOD）活性的影响，利用高效液相色谱法（HPLC）检测广藿香根、茎、叶水提液中原儿茶酸、绿原酸、香草酸、咖啡酸、丁香酸、阿魏酸、苯甲酸和肉桂酸含量，及香草酸、丁香酸、阿魏酸、苯甲酸和肉桂酸对广藿香幼苗的化感作用，以期为广藿香连作障碍的机制及成因提供参考依据。结果表明：广藿香根、茎、叶水提液对广藿香幼苗具有化感自毒作用，且具有浓度效应，叶水提液的化感自毒作用最显著，其酚酸的种类和含量高于根和茎的。叶片水提液中酚酸含量为苯甲酸>原儿茶酸>咖啡酸>阿魏酸>丁香酸>香草酸>绿原酸>肉桂酸。5种酚酸物质对广藿香的株高、根长、根数、鲜质量、叶面积、根系活力、总叶绿素含量均有不同程度的抑制影响，对叶片的POD、CAT、SOD活性及MDA含量有升高的影响，作用均具有浓度效应。5种酚酸对广藿香幼苗的化感作用为肉桂酸>苯甲酸>阿魏酸>丁香酸>香草酸，广藿香的连作障碍与其器官的水溶性酚酸类自毒物质可能有密切关系，肉桂酸、苯甲酸等酚酸类物质可能是其主要的化感自毒物质。
- Abstract:
- Taking the tissue culture acclimated plantlet of Pogostemon cablin as test material.The effects of water extracts from roots,stems and leaves of Pogostemon cablin on root activity,total chlorophyll content,malondialdehyde content,peroxidase (POD) activity,catalase (CAT) activity and superoxide dismutase (SOD) activity of Pogostemon cablin seedlings were studied by pot experiment with tissue culture domesticated seedlings.In addition,high performance liquid chromatography(HPLC) was used to detect the contents of protocatechuic acid,chlorogenic acid,vanillin acid,caffeic acid,eugenic acid,ferulic acid,benzoic acid and cinnamic acid in water extracts from Pogostemon cablin roots,stems and leaves,as well as the allelosensitive effects of vanillin acid,eugenic acid,ferulic acid,benzoic acid and cinnamic acid on Pogostemon cablin seedlings.In order to provide a reference basis for the mechanism and causes of continuous cropping disorders of Pogostemon cablin.The results showed that water extracts from roots,stems and leaves had allelopathic and concentration effects on Pogostemon cablin seedlings,and the allelopathic and self-toxic effects of water extracts from leaves were the most significant,and the types and contents of phenolic acids were higher than those from roots and stems.The content of phenolic acid in the aqueous extract of leaves was as follows,benzoic acid>protocatechuic acid>caffeic acid>ferulic acid>eugenic acid>vanillin acid>chlorogenic acid>cinnamic acid.The five phenolic acids had different inhibitory effects on plant height,root length,root number,fresh weight,leaf area,root activity and total chlorophyll content of Pogostemon cablin,and increased POD,CAT,SOD activities and MDA content of leaves,all of which had concentration effects.The allelopathic effects of the five phenolic acids on the Pogostemon cablin seedlings were compared as follows cinnamic acid>benzoic acid>ferulic acid>syringic acid>vanillic acid.The continuous dysfunction of Pogostemon cablin might be closely related to the water-soluble phenolic acid self-toxic substances in its organs.The phenolic acid substances such as cinnamic acid and benzoic acid might be the main allelopathic self-toxic substances.
参考文献/References:
[1]国家药典委员会.中国药典Ⅰ部[S].北京:中国医药科技出版社,2015.[2]周芳,曹国璠,李金玲,等.药用植物连作障碍机制及其缓解措施研究进展[J].山地农业生物学报,2019,38(3):67-72.[3]张重义,林文雄.药用植物的化感自毒作用与连作障碍[J].中国生态农业学报,2009,17(1):189-196.[4]胡帅珂,高岩,王莘.水稻残茬自毒作用研究[J].现代农业科技,2012(5):51-52．[5]韩丽梅,王树起,鞠会艳,等.大豆根茬腐解产物的鉴定及化感作用的初步研究[J].生态学报,2000,20(5):771-778．[6]沙红,高燕,李雪源.棉花植株水提取液对种子萌发和幼苗生长的自毒作用[J].新疆农业科学,2012,49(5):826-830．[7]黄玉茜，韩立思，杨劲峰,等.花生植株和土壤水浸液自毒作用研究及土壤中自毒物质检测[J].生态学报，2012(19):6023-6032.[8]杨从军.番茄茎叶水提取液对7种蔬菜的化感作用[J].青岛农业大学学报(自然科学版),2019,36(2):91-94,111.[9]张文明,邱慧珍,张春红,等.连作马铃薯不同生育期根系分泌物的成分检测及其自毒效应[J].中国生态农业学报,2015,23(2):215-224．[10]王田涛,王琦,王惠珍,等.当归自毒作用和其对不同作物的化感效应[J].草地学报,2012,20(6):1132-1138．[11]马红梅,赵培芳.灵芝连作障碍下的自毒作用[J].北方园艺,2016(6):133-136．[12]冯静,周冰谦,刘谦,等.药用植物根际微生物与酚酸类自毒物质致害机制及作用关系研究[J].中华中医药杂志,2020,35(8):4049-4052.[13]BOOKER F L,BLUM U,FISCUS E L.Short-term effects of ferulic acid on ion uptake and water relations in cucumber seedlings[J].Journal of Experimental Botany,1992,43(5):649-655.[14]唐堃,李明,董闪,等.广藿香根际土壤水浸液对其扦插苗的化感自毒作用[J].中药材,2014,37(6):935-939.[15]唐堃,李明,赵盼,等.广藿香连作土壤对其扦插苗扦插生根的化感作用[J].广东农业科学,2014,41(6):85-88,237.[16]李玲梅.利用组织培养技术研究广藿香的化感自毒作用[D].广州:广东药学院,2012.[17]宫李辉,高振宇,马伯军,等.水稻粒形遗传的研究进展[J].植物学报,2011,46(6):597-605.[18]吴元华,浦选昌,杨昌元,等.黔东南禾稻类型分类及叶龄栽培模式研究[J].贵州农业科学,2010,38(1):49-52.[19]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.[20]李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2009.[21]李玲,李娘辉,蒋素梅,等.植物生理学模块实验指导[M].北京:科学出版社,2009.[22]王园园,董淑琦,王宏富,等.谷子根系水浸提液的化感自毒效应研究[J].山西农业大学学报(自然科学版),2019,39(2):7-13.[23]黄钰芳,张恩和,张新慧,等.兰州百合根及鳞茎水浸液自毒作用的研究[J].草业学报,2017,26(8):93-103.[24]段修冉,李明,李龙明,等.广藿香不同茬口土壤对其幼苗农艺性状和百秋李醇含量的影响[J].中药材,2017,40(11):2516-2519.[25]唐堃,李明,董闪,等.广藿香根际土壤水浸液对其扦插苗的化感自毒作用[J].中药材,2014,37(6):935-939.[26]唐堃,李明,赵盼,等.广藿香连作土壤对其扦插苗扦插生根的化感作用[J].广东农业科学,2014,41(6):85-88,237.[27]孔垂华,胡飞.植物化感(相生相克)作用及其应用[M].北京:中国农业出版社,2001.[28]吕丰娟,肖运萍,汪瑞清,等.连作对不同抗性芝麻根系分泌物中酚酸类物质含量的影响及其化感作用[J].江西农业学报,2020,32(4):1-8.[29]李敏,闫兴富,马丽,等.酚酸类化感自毒物质对枸杞种子萌发的抑制作用[J].生态学报,2020,40(6):2072-2079.[30]汤绍虎,罗充.植物生物学教程[M].重庆:西南师范大学出版社,2012.
相似文献/References:
[1]江贵波,吴伟珊,林理强,等.三叶草对三叶鬼针草的化感作用[J].北方园艺,2014,38(11):106.
JIANG Gui-bo,WU Wei-shan,LIN Li-qiang,et al.Allelopathy of Oxalis corymbosa DC on Bidens pilosa L.[J].Northern Horticulture,2014,38(16):106.
[2]何梦玲,何芳,熊洋,等.茉莉酸甲酯对广藿香叶中百秋里醇含量的影响[J].北方园艺,2014,38(05):147.
HE Meng-ling,HE Fang,XIONG Yang,et al.Effect of Methyl Jasmonate on the Content of Patchouli Alcohol From the Leaves of Pogostemon cablin (Blanco) Benth.[J].Northern Horticulture,2014,38(16):147.
[3]秦永燕,刘瑞祥,铁军,等.西芹水提液对蚕豆根尖微核率的影响[J].北方园艺,2014,38(20):108.
QIN Yong-yan,LIU Rui-xiang,TIE Jun,et al.Effect of Water Extract of Apium graveolens on Micronucleus Rate of Vicia Faba Root Tip Cells[J].Northern Horticulture,2014,38(16):108.
[4]唐堃,李明,赵盼,等.广藿香挥发油对其组织培养幼苗化感自毒作用的研究[J].北方园艺,2014,38(19):128.
TANG Kun,LI Ming,ZHAO Pan,et al.Allelopathy of Effect Volatile Oil from Pogostemon cablin to Its Tissue Culture Seedlings[J].Northern Horticulture,2014,38(16):128.
[5]谢修鸿,刘玉伟,王晓红,等.孔雀草水提液对自身生长及土壤微生物数量和酶活性的影响[J].北方园艺,2015,39(06):157.[doi:10.11937/bfyy.201506044]
XIE Xiu-hong,LIU Yu-wei,WANG Xiao-hong,et al.Effect of Water Extraction Liquid of Tagetes patula L.on Itself Growth and Microbial Quantity and Enzymatic Activity[J].Northern Horticulture,2015,39(16):157.[doi:10.11937/bfyy.201506044]
[6]严寒静,熊洋,张宏意,等.广藿香八倍体叶片叶绿素荧光的日变化[J].北方园艺,2015,39(24):153.[doi:10.11937/bfyy.201524042]
YAN Hanjing,XIONG Yang,ZHANG Hongyi,et al.Diurnal Changes in Chlorophyll Fluorescence Parameters of Homologous Octoploid Pogostemon cablin Leaves[J].Northern Horticulture,2015,39(16):153.[doi:10.11937/bfyy.201524042]
[7]吴燕燕,李明,黄结雯,等.重茬土壤、枯叶腐解液对广藿香扦插苗生理生化指标的影响[J].北方园艺,2017,41(10):149.[doi:10.11937/bfyy.201710034]
WU Yanyan,LI Ming,HUANG Jiewen,et al.Effect of Continuous Cropping Soil and Decomposed Liquid of Dead Leaves on Physiological and Biochemical Indexes of Pogostemon cablin Cutting Seedlings[J].Northern Horticulture,2017,41(16):149.[doi:10.11937/bfyy.201710034]
[8]常晖,逯莉,党艳妮,等.黄精对三种作物种子萌发和幼苗生长的化感作用[J].北方园艺,2019,43(08):125.[doi:10.11937/bfyy.20182321]
CHANG Hui,LU Li,DANG Yanni,et al.Allelopathic Effect of Polygonatum sibiricum Red.on Seed Germination and Seeding Growth of Three Kinds of Crops[J].Northern Horticulture,2019,43(16):125.[doi:10.11937/bfyy.20182321]
[9]广藿香AFLP反应体系的优化及引物筛选.广藿香AFLP反应体系的优化及引物筛选[J].北方园艺,2020,44(11):117.[doi:10.11937/bfyy.20193400]
LIU Xiaoying,LU Changhua,LI Jiahui,et al.Optimization of AFLP Reaction System and Screening of Primers for Pogostemon cablin[J].Northern Horticulture,2020,44(16):117.[doi:10.11937/bfyy.20193400]
[10]周界,潘丽萍,李明.广藿香间作紫苏对其连作障碍的缓解效应[J].北方园艺,2020,44(13):111.[doi:10.11937/bfyy.20192582]
ZHOU Jie,PAN Liping,LI Ming.Relieving Effects of Pogostemon cablin Intercropping WithPerilla frutescens on Its Continuous Cropping Obstacle[J].Northern Horticulture,2020,44(16):111.[doi:10.11937/bfyy.20192582]
备注/Memo
第一作者简介：卢红（1996-），女，硕士研究生，研究方向为中药资源开发与品质评价。E-mail:1064174597@qq.com.责任作者：李明（1963-），女，博士，教授，现主要从事药用植物资源及质量评价等研究工作。E-mail:13539843803@163.com.基金项目：广东省科技计划资助项目（2013B020503066,2014A020208135）；广东省普通高校重点领域专项资助项目（乡村振兴）（2020ZDZX1055）；国家重点研发计划资助项目（2017YFC1700704）。收稿日期：2021-01-05