ZHOU Jie,PAN Liping,LI Ming.Relieving Effects of Pogostemon cablin Intercropping WithPerilla frutescens on Its Continuous Cropping Obstacle[J].Northern Horticulture,2020,44(13):111-117.[doi:10.11937/bfyy.20192582]
广藿香间作紫苏对其连作障碍的缓解效应
- Title:
- Relieving Effects of Pogostemon cablin Intercropping WithPerilla frutescens on Its Continuous Cropping Obstacle
- 文献标志码:
- A
- 摘要:
- 以广藿香组培苗和紫苏苗为试材,采用室内盆栽的方法,分别设定3个不同种植方式处理(连作组(第2茬广藿香单作)、间作组(第2茬广藿香与紫苏间作)和对照组(第1茬广藿香单作)),通过测定广藿香农艺性状、叶片丙二醛(MDA)含量、根际土壤微生物数量与土壤酶活性、药材中百秋李醇和广藿香酮含量,研究间作紫苏的种植方式对广藿香连作障碍的缓解效应。结果表明:培育90 d后,广藿香农艺性状(株高、干质量、鲜质量、主根长)及百秋李醇和广藿香酮的含量,间作组显著高于连作组(P<0.05),而与对照组无显著性差异。叶片MDA含量,间作组较连作组减少了33.74%(P<0.05),而与对照组无显著性差异。土壤细菌和放线菌数量,间作组较连作组分别增加了30.35%、66.67%(P<0.05),间作组较对照组分别增加了21.24%、75.00%(P<0.05)。土壤真菌数量,间作组与对照组无显著性差异。土壤蔗糖酶、脲酶和酸性磷酸酶活性,间作组较连作组分别升高了25.50%、30.58%和50.22%(P<0.05),而土壤蔗糖酶活性,间作组与对照组无显著性差异,脲酶和酸性磷酸酶活性,间作组较对照组分别升高了7.44%、41.92%(P<0.05)。综上所述,广藿香间作紫苏可在一定程度上缓解广藿香连作障碍。
- Abstract:
- Taking Pogostemon cablintissue culture seedlings and Perilla frutescens seedlings as materials and adopting a method of the pot culture in house,there were 3 treatments,continuous cropping group(only the second crop Pogostemon cablin),intercropping group(the second crop Pogostemon cablin intercropping with perilla frutescens),control group(only the first crop Pogostemon cablin),and the agronomic traits,the content of malondialdehyde (MDA) in leaves,microbial quantities and soil enzyme activities in rhizosphere soil,the content of patchouli alcohol and pogostone in Pogostemon cablin were determined,in order to study the relieving effect of Pogostemon cablin intercropping with Perilla frutescens on its continuous cropping obstacle.The results showed that after 90 days of cultivation,the agronomic traits (plant height,dry weight,fresh weight,main root length) and the content of patchouli alcohol and pogostone were significantly higher in the intercropping group than in the continuous cropping group (P<0.05),and there were no significant differences between intercropping group and control group.For the content of MDA in the leaves,intercropping group was reduced by 33.74% (P<0.05) compared with the continuous cropping group,but there was no significant difference between intercropping group and control group.Compared with the continuous cropping group,the number of soil bacteria and actinomycetes in Intercropping group increased by 30.35% and 66.67% (P<0.05),respectively,and the intercropping group increased by 21.24% and 75.00%(P<0.05),respectively,compared with the control group.There was no significant difference in the number of soil fungi between the intercropping group and the control group.The activities of soil invertase,urease and acid phosphatase in intercropping group were increased by 25.50%,30.58% and 50.22% (P<0.05),respectively than the continuous cropping group.The soil invertase activity was not significant difference between the intercropping group and the control group.While urease and acid phosphatase activities in intercropping group were increased by 7.44% and 41.92% (P<0.05),respectively,compared with the control group.It was concluded that the intercropping of Perilla frutescens could alleviate the continuous cropping obstacle in Pogostemon cablin to a certain extent.
参考文献/References:
[1]国家药典委员会.中国药典:一部[M].北京:中国医药科技出版社,2015:45.[2]胡海燕,彭劲甫,黄世亮,等.分子蒸馏技术用于广藿香油纯化工艺的研究[J].中国中药杂志,2004(4):36-38.[3]卢丽兰,杨新全,王彩霞,等.不同硝铵比氮素供应对广藿香生长及药效成分的影响[J].植物营养与肥料学报,2017,23(5):1314-1325.[4]SINGH M,GANESHA R R S.Influence of sources and doses of N and K on herbage,oil yield and nutrient uptake of patchouli [Pogostemon cablin (Blanco) Benth.] in semi-arid tropics[J].Industrial Crops & Products,2008,29(1):229-234.[5]DONELIAN A,CARLSON L H,LOPES T J,et al.Comparison of extraction of patchouli (Pogostemon cablin) essential oil with supercritical CO2 and by steam distillation[J].Journal of Supercritical Fluids,2009,48(1):15-20.[6]梁开明,章家恩,杨滔,等.水稻与慈姑间作栽培对水稻病虫害和产量的影响[J].中国生态农业学报,2014,22(7):757-765.[7]牛宇,薄晓峰,秦作霞,等.间作豆类作物对大樱桃生长和土壤特性、微生物数量的影响[J].北方园艺,2019(4):38-44.[8]何国振,李锦坤,高伟,等.广东省广藿香种植业发展策略[J].中国农学通报,2012,28(31):288-292.[9]李玲梅.利用组织培养技术研究广藿香的化感自毒作用[D].广州:广东药学院,2012.[10]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[11]刘素慧.大蒜连作障碍形成机理及EM缓解效应的研究[D].泰安:山东农业大学,2011.[12]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.[13]张亚楠,李孝刚,王兴祥.茅苍术间作对连作花生土壤线虫群落的影响[J].土壤学报,2016,53(6):1497-1505.[14]张芸,李龙,李强,等.不同间作方式对蚕豆农艺性状的影响[J].农业科技通讯,2018(9):109-111.[15]汤绍虎,罗充.植物生物学教程[M].重庆:西南师范大学出版社,2012.[16]安艳,杨丹,李鑫,等.半夏连作障碍效应及生理机制研究[J].西北农业学报,2018,27(7):1017-1022.[17]焦润安,徐雪风,杨宏伟,等.连作对马铃薯生长和土壤健康的影响及机制研究[J].干旱地区农业研究,2018,36(4):94-100.[18]杨宏伟,李自龙,梁恕坤,等.间作百喜草对油橄榄根际微环境及抗旱生理的影响[J].应用与环境生物学报,2016,22(3):455-461.[19]张海春,张浩,胡晓辉.不同间作模式对温室连作番茄产量、土壤微生物和酶的影响[J].西北农业学报,2016,25(8):1218-1223.
相似文献/References:
[1]刘晓莉,田海娟,钟容,等.盐沉淀法提取紫苏活性蛋白的工艺研究[J].北方园艺,2013,37(04):147.
[2]何梦玲,何芳,熊洋,等.茉莉酸甲酯对广藿香叶中百秋里醇含量的影响[J].北方园艺,2014,38(05):147.
HE Meng-ling,HE Fang,XIONG Yang,et al.Effect of Methyl Jasmonate on the Content of Patchouli Alcohol From the Leaves of Pogostemon cablin (Blanco) Benth.[J].Northern Horticulture,2014,38(13):147.
[3]周秀梅,关晓弯,冯晓燕,等.紫苏地上器官水浸液对野草香的化感作用[J].北方园艺,2012,36(22):50.
ZHOU Xiu-mei,GUAN Xiao-wan,FENG Xiao-yan,et al.Allelopathy of Aqueous Extracts from Different Up-ground Organs of Perilla frutescens on Elsholtzia cypriani[J].Northern Horticulture,2012,36(13):50.
[4]唐堃,李明,赵盼,等.广藿香挥发油对其组织培养幼苗化感自毒作用的研究[J].北方园艺,2014,38(19):128.
TANG Kun,LI Ming,ZHAO Pan,et al.Allelopathy of Effect Volatile Oil from Pogostemon cablin to Its Tissue Culture Seedlings[J].Northern Horticulture,2014,38(13):128.
[5]向 福,程 超,何乾坤,等.油茶叶及凋落物浸提液对紫苏幼苗生长的化感作用[J].北方园艺,2015,39(12):1.[doi:10.11937/bfyy.201512001]
XIANG Fu,CHENG Chao,HE Qiankun,et al.Allelopathy of Aqueous Extracts from Camellia oleifera Leaf and Litter Fall on Seedling Growth of Perilla frutescens[J].Northern Horticulture,2015,39(13):1.[doi:10.11937/bfyy.201512001]
[6]邓 芸,盖 琼 辉.紫苏 · 香紫苏 · 东紫苏的比较研究[J].北方园艺,2010,34(04):0.[doi:10.11937/bfyy.201004078]
DENG Yun,GAI Qiong - hui.The Comparative Study of Perilla, Salvia sclarea ,and Elshdtzia bodinieri[J].Northern Horticulture,2010,34(13):0.[doi:10.11937/bfyy.201004078]
[7]杜云安,李泓达,向国红,等.紫苏生物学特性的研究[J].北方园艺,2015,39(21):41.[doi:10.11937/bfyy.201521011]
DU Yunan,LI Hongda,XIANG Guohong,et al.Research on Perilla frutescens Biological Characteristics[J].Northern Horticulture,2015,39(13):41.[doi:10.11937/bfyy.201521011]
[8]严寒静,熊洋,张宏意,等.广藿香八倍体叶片叶绿素荧光的日变化[J].北方园艺,2015,39(24):153.[doi:10.11937/bfyy.201524042]
YAN Hanjing,XIONG Yang,ZHANG Hongyi,et al.Diurnal Changes in Chlorophyll Fluorescence Parameters of Homologous Octoploid Pogostemon cablin Leaves[J].Northern Horticulture,2015,39(13):153.[doi:10.11937/bfyy.201524042]
[9]黄 美 娟,张 蕾,黄 海 泉.紫苏的组织培养及快繁技术研究[J].北方园艺,2008,32(01):0.[doi:10.11937/bfyy.200801084]
HUANG Mei -juan,ZHANG Lei,HUANG Hai -quan.Studies on Tissue Culture and Rapid Propagation of Perilla frutescens ( Linn) Britt[J].Northern Horticulture,2008,32(13):0.[doi:10.11937/bfyy.200801084]
[10]吴燕燕,李明,黄结雯,等.重茬土壤、枯叶腐解液对广藿香扦插苗生理生化指标的影响[J].北方园艺,2017,41(10):149.[doi:10.11937/bfyy.201710034]
WU Yanyan,LI Ming,HUANG Jiewen,et al.Effect of Continuous Cropping Soil and Decomposed Liquid of Dead Leaves on Physiological and Biochemical Indexes of Pogostemon cablin Cutting Seedlings[J].Northern Horticulture,2017,41(13):149.[doi:10.11937/bfyy.201710034]
备注/Memo
第一作者简介:周界(1994-),男,硕士研究生,研究方向为中药资源开发与品质评价。E-mail:15707489965@163.com.责任作者:李明(1963-),女,博士,教授,现主要从事药用植物资源及质量等研究工作。E-mail:13539843803@163.com.基金项目:国家重点研发计划资助项目(2017YFC1700704);广东省科技计划资助项目(2013B020503066,2014A020208135,2016A020226050)。收稿日期:2019-09-18