[1]孔珊珊,王芳,朱绍志,等.羧甲基壳聚糖处理对采后蓝莓抗灰霉病的影响[J].北方园艺,2020,44(02):15-22.[doi:10.11937/bfyy.20191891]
　KONG Shanshan,WANG Fang,ZHU Shaozhi,et al.Effect of Carboxymethyl Chitosan Treatment on Postharvest Blueberry Resistance to Gray Mold[J].Northern Horticulture,2020,44(02):15-22.[doi:10.11937/bfyy.20191891]
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羧甲基壳聚糖处理对采后蓝莓抗灰霉病的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年02 页码: 15-22 栏目: 出版日期: 2020-01-30
Title:
Effect of Carboxymethyl Chitosan Treatment on Postharvest Blueberry Resistance to Gray Mold
作者:
孔珊珊1; 王芳2; 朱绍志1; 李文清1; 李贤忠1; 邓佳1; 3
（1.西南林业大学 林学院，云南 昆明 650224；2.西南林业大学 国家林业局西南地区生物多样性保育重点实验室，云南 昆明 650224；3.西南林业大学 西南山地森林资源保育利用省部共建教育部重点实验室，云南 昆明 650224）
Author(s):
KONG Shanshan1; WANG Fang2; ZHU Shaozhi1; LI Wenqing1; LI Xianzhong1; DENG Jia1; 3
(1.Academy of Forestry，Southwest Forestry University，Kunming,Yunnan 650224；2.Key Laboratory of State Forestry Administration for Biodiversity Conservation in Southwest China，Southwest Forestry University，Kunming,Yunnan 650224；3.Key Laboratory of Ministry of Education for Forest Resources Conservation and Utilization in the Southwest Mountains of China，Southwest Forestry University，Kunming,Yunnan 650224)
关键词:
蓝莓; 羧甲基壳聚糖; 诱导抗病; 酶活性; 果实品质
Keywords:
blueberry; carboxymethyl chitosan; induced disease resistance; enzyme activity; fruit quality
DOI:
10.11937/bfyy.20191891
文献标志码:
A
摘要:
以“灿烂”蓝莓为试材，研究不同浓度的羧甲基壳聚糖处理对采后果实灰霉病、抗病防御酶活性和果实品质的影响,以期为羧甲基壳聚糖在果实采后病害防治、贮藏保鲜应用提供参考依据。结果表明：1.5%和2.0%羧甲基壳聚糖处理能有效抑制采后蓝莓灰霉病发生、诱导果实抗病能力且维持蓝莓果实采后营养品质。体外培养条件下，羧甲基壳聚糖处理可明显抑制灰霉菌落生长、抑制体外灰霉孢子的萌发及芽孢管的生长，2.0%羧甲基壳聚糖抑制效果较显著（P<0.05）。对不同浓度羧甲基壳聚糖浸泡处理的蓝莓果实接种灰霉孢子悬浮液，发现果实发病率随羧甲基壳聚糖浓度的升高而降低，羧甲基壳聚糖有效抑制了采后灰霉病的发生。常温贮藏条件下，不同浓度羧甲基壳聚糖处理显著（P<0.05）提高了果实多酚氧化酶（PPO）、过氧化物酶（POD）、β-1,3-葡聚糖酶（GLU）和几丁质酶（CHT）的活性，贮藏后期，2%羧甲基壳聚糖诱导抗病性效果最好。此外，羧甲基壳聚糖处理能明显保持贮藏期蓝莓果实品质，果实失重率显著下降，糖酸含量的损失明显减小，可溶性固形物含量维持较高水平。
Abstract:
The effects of different concentrations of carboxymethyl chitosan on gray mold incidence rate，disease resistance enzyme activity and fruit quality of postharvest fruits were studied with ‘Bright’ blueberries as test materials in order to provide reference for preservation of blueberry after harvest.The results showed that 1.5% and 2.0% carboxymethyl chitosan treatment could effectively inhibit the occurrence of postharvest blueberry gray mold，induce disease resistance and maintain the nutritional quality of blueberry fruits.Under in vitro culture conditions，carboxymethyl chitosan could significantly inhibit the growth of B.cinerea colonies，the spores germination，the spores growth，and the inhibition of 2.0% carboxymethyl chitosan was more significant (P<0.05).The blueberry fruit dipped with different concentrations carboxymethyl chitosan was inoculated with the spore suspension of B.cinerea，and the gray mold disease incidence of fruit decreased with the increase of the concentration of carboxymethyl chitosan.Under the ambient temperature storage conditions，the treatments with different concentrations carboxymethyl chitosan solution significantly (P<0.05) increased the activities of polyphenol oxidase (PPO)，peroxidase (POD)，β-1,3-glucan (GLU) and chitinase (CHT) of blueberry fruit，2.0% of carboxymethyl chitosan had the best effect in inducing disease resistance in the late storage period.In addition，carboxymethyl chitosan treatment could obviously maintain the quality of blueberry fruits during storage，the fruit weight loss rate，the loss of sugar and acid content was significantly reduced，and the content of soluble solids was maintained at a high level.

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备注/Memo

第一作者简介：孔珊珊（1993-），女，硕士研究生，研究方向为果品贮藏与加工。E-mail:2535355481@qq.com.责任作者：邓佳（1983-），女，博士，副教授，现主要从事果树培育与利用等研究工作。E-mail:dengjia1983@163.com.基金项目：国家自然科学基金资助项目（31460214，31960326）；云南省教育厅基金资助项目（2018JS327）。收稿日期：2019-08-05

更新日期/Last Update: 2020-02-22