FU Bing,HONG Zhen,LIU Yue-jun,et al.Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity[J].Northern Horticulture,2013,37(17):95-99.
蓝莓SRAP-PCR反应体系的建立和优化及其遗传多样性分析
- Title:
- Optimization and Establishment of SRAP-PCR Reaction System of Vaccinium ssp. and Analysis of the Genetic Diversity
- 文章编号:
- 1001-0009(2013)17-0095-05
- Keywords:
- blueberry; SRAP-PCR; optimization; genetic diversity
- 分类号:
- S 663.9
- 文献标志码:
- A
- 摘要:
- 以9个品种蓝莓为试材，采用L16（45）正交实验设计建立和优化了蓝莓SRAP-PCR反应体系，并利用该体系分析了供试蓝莓品种之间的遗传多样性。结果表明：蓝莓SRAP-PCR反应的最佳体系（20 μL）为：MgCl2 2.5 mmol/L、dNTPs 100 μmol/L、Taq DNA聚合酶0.8 U、引物0.2 μmol/L、DNA 40 ng、1×Reaction Buffer;9个品种蓝莓的遗传相似性系数分布于0.1818~1.0000之间，平均遗传相似性系数为0.6359，存在较高的遗传多样性。基于UPGMA的聚类分析将供试材料分成4个类群，主坐标分析同样将供试材料分成4个类群，二者结果一致。
- Abstract:
- Taking 9 varieties of blueberry as material,the SRAP-PCR reaction system of Vaccinium ssp. was established and optimized by L16(45) orthogonal experimental design.The genetic diversity of them was analyzed by the optimal reaction system.The results showed that the optimal and stable SRAP-PCR system was as follows:2.5 mmol/L Mg2+,100 μmol/L dNTPs,0.8 U Taq polymerase,0.2 μmol/L primers and 40 ng DNA template,1×Reaction Buffer,within total 20 μL reaction solution.The GS coefficients among the 9 varieties of blueberry were ranged from 0.1818 to 1.0000 with an average of 0.6359,this suggested that the genetic variation was high.These varieties were divided into four groups on the UPGMA dendrogram constructed from GS coefficients,and also divided into four groups by the method of principal coordinates analysis.The UPGMA and GS coefficients results was the same.
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备注/Memo
第一作者简介：傅冰（1982-），男，浙江松阳人，硕士，讲师，现主要从事生物化学与分子生物学等研究工作。E-mail:41891514@qq.com.
责任作者：洪震（1969-），男，浙江丽水人，副教授，现主要从事植物开发利用等研究工作。
收稿日期：2013-04-09