[1]甘林鑫,李厚华,杨晨,等.不同花色牡丹品种花瓣色素含量及成分分析[J].北方园艺,2020,44(05):67-73.[doi:10.11937/bfyy.20191432]
　GAN Linxin,LI Houhua,YANG Chen,et al.Analysis of the Content and Composition of Peony Pigments in Different Colors[J].Northern Horticulture,2020,44(05):67-73.[doi:10.11937/bfyy.20191432]
点击复制

不同花色牡丹品种花瓣色素含量及成分分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年05 页码: 67-73 栏目: 出版日期: 2020-03-15
Title:
Analysis of the Content and Composition of Peony Pigments in Different Colors
作者:
甘林鑫; 李厚华; 杨晨; 明莉; 韩美玲; 辛转霞
（西北农林科技大学 风景园林艺术学院，陕西 杨凌 712100）
Author(s):
GAN Linxin; LI Houhua; YANG Chen; MING Li; HAN Meiling; XIN Zhuanxia
(School of Landscape Architecture，Northwest A&F University，Yangling，Shaanxi 712100)
关键词:
牡丹; 色素; 花青苷; 类黄酮
Keywords:
peony; pigment; anthocyanin; flavonoids
DOI:
10.11937/bfyy.20191432
文献标志码:
A
摘要:
以分属白、粉、红3个色系的6个牡丹品种花瓣为试材，采用薄层层析色谱法(TLC)、紫外-可见分光光度计(UV)和高效液相色谱仪-二极管阵列检测器(HPLC-DAD)研究不同花色牡丹品种花瓣中的色素含量，并进行了定性分析。结果表明：粉色和红色系牡丹中检测出2种花青苷，分别是矢车菊素-3,5-二葡糖苷和芍药花素-3,5-二葡糖苷，白色系牡丹中未检测到花青苷类物质，其中矢车菊素-3,5-二葡糖苷在粉色系牡丹中含量较高，而红色系中总黄酮和总花青苷的含量最高。
Abstract:
The petals of 6 peony varieties belonging to three color series of white，pink and red were used as test materials.Thin layer chromatography (TLC)，ultraviolet-visible spectrophotometer (UV)， and high-performance liquid chromatography-diode were used.Array detector (HPLC-DAD) was used to study the pigment content in the petals of different peony varieties，and a qualitative analysis was performed.The results showed that two anthocyanins were detected in pink and red peony，namely cyanidin-3,5-diglucoside and paeoniflorin-3,5-diglucoside，and no white peony was detected.Anthocyanins，including cyanidin-3,5-diglucoside，were higher in the pink peony，while the total flavonoids and total anthocyanins were the highest in the red.

参考文献/References:

[1]罗宁.充分认识牡丹价值,发展牡丹事业[J].中国园林,2012,28(3):99-101.[2]程金水,刘青林.园林植物育种学[M].北京:中国林业出版社,2010.[3]王亮生.ボタンの花色とフラボノイド色素に関する研究[D].日本鹿児島:鹿児島大学,2001.[4]HARBORNE,JEFFREY B.Phytochemical methods：A guide to modern techniques of plant analysis[M].USA:Chapman And Hall,1984[5]SHASHANK K,ABHAY K,PANDEY K.Chemistry and biological activities of flavonoids:An overview[J/OL].The Scientific World Journal,2013,DOI:10.1155/2013/162750.[6]蔡雪雁,李厚华,李玲.紫叶桃叶片色素的定性及定量分析[J].西北林学院学报,2015，30(3):206-210.[7]高俊凤.植物生理学实验技术[M].西安:世界图书出版社,2000.[8]杨琴,袁涛,孙湘滨.两个牡丹品种开花过程中花色变化的研究[J].园艺学报,2015,42(5):930-938.[9]KUEHNLE A R,LEWIS D H,MARKHAM K R,et al.Floral flavonoids and pH in Dendrobium orchid species and hybrids[J].Euphytica,1997,95(2):187-194.[10]徐有朋.木材学[M].北京:中国林业出版社,2006.[11]刘传菊.杨梅中酚类物质的分离、分析、特性及应用研究[D].武汉:华中农业大学,2008.[12]钟培星,王亮生,李珊珊.芍药开花过程中花色和色素的变化[J].园艺学报,2012,39(11):2271-2282.[13]李想,段晶晶,罗小宁.依据理化性质分析牡丹花色形成的影响因素[J].东北林业大学学报,2019,47(3):38-43,67.[14]李盈侠,刘红艳,李真.牡丹观赏品种花色表型分析[J].辽宁林业科技,2018(4):1-6.[15]华梅,原晓龙,杨卫.HPLC分析6种不同花色滇牡丹花瓣中花青素和黄酮[J].西部林业科学,2017,46(6):40-45.[16]王若晗,丁建兰.牡丹的花型和花色[J].中国花卉园艺,2017(2):22-23.[17]张宝智.江南牡丹花色素组成与开花过程生理特征研究[D].上海:上海交通大学,2013.[18]韩江南.牡丹花色与花色苷的研究[D].洛阳:河南科技大学,2010.[19]陈耀华.牡丹的花色与品种分类[J].花木盆景(花卉园艺),2001(3):14.

相似文献/References:

[1]郭丽丽,李 军,李金航,等.不同时期盆栽牡丹叶片RNA的提取研究[J].北方园艺,2014,38(12):99.
　GUO Li-li,LI Jun,LI Jin-hang,et al.Extraction of Total RNA From Potted Tree Peony Leaves at Different Stages[J].Northern Horticulture,2014,38(05):99.
[2]刘 娜,秦安臣,陈 雪,等.牡丹对B9花期调控的响应研究[J].北方园艺,2014,38(10):56.
　LIU Na,QIN An-chen,CHEN Xue,et al.Study on the Peony’s Flowering Regulation Response to B9[J].Northern Horticulture,2014,38(05):56.
[3]闻月冰,邹 宇,胡立明,等.紫甘蓝色素提取条件优化及其稳定性研究[J].北方园艺,2014,38(10):127.
　WEN Yue-bing,ZOU Yu,HU Li-ming,et al.Optimized Extraction Conditions and Stability of Purple Cabbage Pigment[J].Northern Horticulture,2014,38(05):127.
[4]杨德翠,郑国生.柱枝孢叶斑病侵染对牡丹生理特性的影响[J].北方园艺,2014,38(01):57.
　YANG De-cui,ZHENG Guo-sheng.Effects of Infection by Cylindrocladium canadenseon Physiological Characteristics of Tree Peony[J].Northern Horticulture,2014,38(05):57.
[5]张永福,吕金富,韩丽,等.凤仙花属不同花色株系间色素及营养物质含量的差异性研究[J].北方园艺,2013,37(14):77.
　ZHANG Yong-fu,LV Jin-fu,HAN Li,et al.Study on Differences of Pigment and Nutrient Substance Among Different Flower’s Colour Strains of Impatiens L.[J].Northern Horticulture,2013,37(05):77.
[6]高宏秀,张莹.牡丹春节催花技术[J].北方园艺,2014,38(06):72.
　GAO Hong-xiu,ZHANG Ying.Flower Forcing Techniques of Tree Peony in Winter[J].Northern Horticulture,2014,38(05):72.
[7]郑玲,程彦伟.六个洛阳牡丹品种解剖结构与抗旱性关系研究[J].北方园艺,2013,37(01):65.
　ZHENG Ling,CHENG Yan-wei.Study on Relationship Between Anatomical Structures and Drought Resistance of Some Cultivars of Luoyang Paeonia suffruticosa Andr.[J].Northern Horticulture,2013,37(05):65.
[8]史倩倩,王雁,周琳,等.生根粉及不同基质对牡丹传统品种扦插的影响[J].北方园艺,2012,36(01):91.
　SHI Qian-qian,WANG Yan,ZHOU Lin,et al.Influence of Rooting Powder and Culture Substrata on Rooting of Different Traditional Cultivars of Paeonia suffruticosa[J].Northern Horticulture,2012,36(05):91.
[9]王 玮,张旭东,施 江,等.不同品种牡丹萌芽过程中组织抗冷性的变化[J].北方园艺,2013,37(06):52.
　WANG Wei,ZHANG Xu-dong,SHI Jiang,et al.Changes of Cold Resistance of Floral Organs During Sprout in Different Tree Peony Cultivars[J].Northern Horticulture,2013,37(05):52.
[10]豆康宁,李玉兰,王富刚.乌饭树叶色素稳定性研究[J].北方园艺,2013,37(19):38.
　DOU Kang-ning,LI Yu-lan,WANG Fu-gang.Study on the Stabilization of Pigment Extracted from Vaccinium bracteatum Thumb.Leaves[J].Northern Horticulture,2013,37(05):38.

备注/Memo

第一作者简介：甘林鑫（1994-），女，硕士研究生，研究方向为园林植物。E-mail：597807188@qq.com.责任作者：李厚华（1973-），男，博士，教授，博士生导师，现主要从事园林植物资源与应用等研究工作。E-mail：lihouhua73@163.com.基金项目：林业公益性行业科研重大专项资助项目（201404701）;国家自然科学基金资助项目（31570697）。收稿日期：2019-07-09

更新日期/Last Update: 2020-04-23