CHEN Liang,CHEN Nianlai,SUN Xiaomei,et al.Identification of Resistance to Fusarium Wilt of Watermelon Grafted Rootstocks and Hybrid[J].Northern Horticulture,2019,43(15):15-22.[doi:10.11937/bfyy.20184314]
西瓜嫁接砧木资源及其杂交组合枯萎病抗性鉴定
- Title:
- Identification of Resistance to Fusarium Wilt of Watermelon Grafted Rootstocks and Hybrid
- Keywords:
- watermelon; Fusarium wilt; physiological race; rootstock materials; artificial inoculation
- 文献标志码:
- A
- 摘要:
- 为了筛选西瓜枯萎病抗性砧木材料，对近年来收集整理的部分西瓜亲本材料及选配的砧木组合进行苗期枯萎病抗性人工接种鉴定。以国际上公认的5个西瓜枯萎病鉴别寄主材料（‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’‘PI296341-FR’）以及选配的49份西瓜砧木资源为试材，采用尖镰孢菌（Fusarium oxysporum f.sp.niveum）孢子悬浮液人工接种法，鉴定试验所用的西瓜枯萎病菌生理小种和砧木材料的枯萎病抗性。结果表明：鉴别寄主‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’ ‘PI296341-FR’的病情指数分别达85.2、53.0、27.2、19.2和7.8，抗性级别分别为感病、轻抗、中抗和抗病；49份西瓜砧木材料中，筛选出对Race 1表现抗病的材料有10份，占总供试材料的20.4%，中抗材料29份，占59.2%，轻抗材料10份，占20.4%，不存在免疫和完全感病的材料。确认该试验所用尖孢镰刀菌为生理小种1，筛选确定了高抗西瓜枯萎病的4份亲本材料588、893、BEH和FR328，6份杂交材料威砧101、Be-3×893、(BEH-15×Sugarlee)×893、(BEH1-15×Sugarlee)×341、(BEH1-15×AW2-1)×341和(BYH-1×Sugarlee)×893，可为设施西瓜生产提供优良的嫁接砧木资源。
- Abstract:
- To screen the resistance to wilt disease of rootstock resources of watermelon,the resistance to wilt disease of some the watermelon parent materials and their hybrid rootstock combinations were identified by artificial inoculation method in seedling stage.The internationally recognized five watermelon wilt identification host materials(‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’‘PI296341-FR’) and the selected 49 rootstock resources of watermelon were identified by Fusarium oxysporum f.sp.niveum spore suspension methods.The results showed that the DI of‘Black Diamond’‘Crimson Sweet’‘Charleston Gray’‘Calhoun Gray’‘PI296341-FR’ was 85.2,53.0,27.2,19.2 and 7.8 respectively,the resistance levels were susceptibility,light resistance,moderate resistance and resistance.10 watermelon rootstock materials(about 20.4% resources) showed resistance to Race 1,29 materials(about 59.2% resources)showed moderate resistance,10 materials(about 20.4% resources) showed light resistance,the immunization and high susceptible resources was not obtained.The results showed that the watermelon wilt pathogen was Race 1 in this experiment,4 parental materials 588,893,BEH and FR328,6 hybrid materials Wei-zhen101,Be-3×893,(BEH-15×Sugarlee)×893,(BEH1-15×Sugarlee)×341,(BEH1-15×AW2-1)×341 and (BYH-1×Sugarlee)×893 showed high resistance to watermelon wilt,and they could be used as excellent grafting rootstock resources for facility watermelon production in future.
参考文献/References:
[1]谭素英,贾文海.无籽西瓜栽培与育种[M].北京:中国农业出版社,2000,40-90.[2]马跃.2007年全国西瓜生产回顾与2008年产销预测[J].长江蔬菜,2008,23(1):54-56.[3]张友青.设施西瓜主要病害特征及防治技术[J].河北农业,2017(7):48-50.[4]ZHANG Z,ZHANG J,WANG Y,et al.Molecular detection of Fusarium oxysporum f.sp.niveum and Mycosphaerella melonis infected plant tissues and soil[J].FEMS Microbiol Lett,2005,249(1):39-47.[5]张屹,魏林,许勇,等.西瓜枯萎病的研究进展[J].湖南农业科学,2013(5):67-70.[6]赵宣.西瓜枯萎病的研究进展[J].农村经济与科技,2018,29(2):26-29.[7]肖光辉.西瓜抗枯萎病育种研究进展[J].湖南农业科学,2001(5):43-45.[8]羊杏平.西瓜枯萎病抗性基因遗传分析、分子标记及育种研究[D].南京:南京农业大学,2012.[9]耿丽华,郭绍贵,吕桂云,等.西瓜枯萎病菌生理小种鉴定技术体系的建立和验证[J].中国蔬菜,2010(20):52-56.[10]林柏青,张松林.辣椒品种抗疫病鉴定方法初步研究[J].中国蔬菜,1994(4):21-24.[11]潘存祥.西瓜抗枯萎病生理生化机理的研究[D].兰州:甘肃农业大学,2014.[12]MARTYN R.An aggressive race of Fusarium oxysporum f.sp. niveum new to the United States[J].Plant Disease,1985,69:1007.[13]MARTYN R D,MCLAUGHLIN R J.Effects of inoculum concentration on the apparent resistance of watermelons to Fusarium oxysporum f.sp.niveum[J].Plant Disease,1983,67：493-495.[14]ZHANG X W,HUANG X S,GU Q S.A preliminary report on screening the resistance of watermelon varieties to Fusarium wilt[J].Acta Hort,1995,402:45-47.[15]段会军,马峙英,张彩英,等.河北省西瓜枯萎病菌生理小种分化及其分布[D].保定:河北农业大学,2005.[16]顾卫红,王燕华,宋荣浩.上海地区西瓜枯萎病病原菌生理小种初探[J].上海农业学报,1994(3):63-67.[17]王冬梅,王纯利,曲丽红,等.新疆西瓜枯萎病菌生理小种初探[J].新疆农业大学学报,1998,21(2):115-118.[18]张兴平,王鸣.我国西瓜枯萎病生理小种分化研究初报[J].中国西瓜甜瓜,1991(1):39-43.[19]NETZER.Inheritance of resistance in watermelon to races of Fusarium oxysporurn[J].Plant Disease,1980,164(9):853-854.[20]CRALL.Historyand present status of water melon improvement by breeding[J].Soil Sci,1953,13:71-74.[21]HENDERSON.The inheritance of Fusarium wilt resistance in watermelon[J].Jam Soc Hort Sci,1970,95:276-282.[22]黄学森,张学炜,焦定量,等.西瓜部分品种抗枯萎病特性在F1代中遗传表现的初步探讨[J].中国西瓜甜瓜,1991(2):11-15.[23]于利,徐润芳,赵有为.西瓜品种抗枯萎病遗传研究[J].江苏农业学报,1995,11(1):45-48.[24]周凤珍,康国斌.西瓜抗枯萎病品种“卡红”的抗病遗传研究[J].植物病理学报,1996,26(3):261-262.
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备注/Memo
第一作者简介：陈亮(1989-)，男，甘肃武威人，硕士，研究实习员，现主要从事设施蔬菜生理与栽培技术等研究工作。E-mail:chenliang144358@163.com.责任作者：李金霞（1985-），女，甘肃天祝人，硕士，讲师，现主要从事作物生理生态学等研究工作。E-mail：lijinxia10000@163.com.基金项目：甘肃省科技厅青年资助项目（18JR3RA187）。收稿日期：2019-03-19