SHI Lingyan,LIU Baowei,GU Xinying,et al.Application of Biochemical and ISSR Molecular Markers in Identification of Auricularia auricular[J].Northern Horticulture,2019,43(09):136-141.[doi:10.11937/bfyy.20183387]
生化和ISSR分子标记在黑木耳品种鉴定中的应用
- Title:
- Application of Biochemical and ISSR Molecular Markers in Identification of Auricularia auricular
- Keywords:
- Auricularia auricula; antagonistic test; esterase isozyme; ISSR
- 文献标志码:
- A
- 摘要:
- 以生产收集的24个黑木耳栽培菌株为试材，采用拮抗、酯酶同工酶及ISSR分子标记技术对其进行分类鉴定和遗传多样性分析。结果表明：酯酶同工酶酶谱显示24个黑木耳菌株共扩增出11条迁移率不同的酶带，聚类分析表明当遗传系数为0.74时，将24个黑木耳菌株分为两大类；通过ISSR分子标记共扩增出67条清晰的DNA多态片段，大小介于0.2~2.0 kb，聚类分析表明当遗传系数为0.85时，将24个菌株分为两大类，聚类分析结果与拮抗试验结果基本一致。该研究为黑木耳的菌株选择及遗传育种提供了参考依据。
- Abstract:
- The antagonistic test，esterase isozyme and ISSR molecular marker technique were performed to analyze the genetic diversity of twenty-four cultivated strains of Auricularia auricula.The results showed that eleven distinct bands were detected by the esterase isozyme.Cluster analysis results showed that the twenty-four cultivated strains of Auricularia auricula were divided into two groups at genetic similarity of 0.74.Sixty-seven distinct bands which ranged from 0.2 kb to 2.0 kb were detected by gel electrophoresis.Cluster analysis results showed the twenty-four cultivated strains of Auricularia auricula were divided into two groups at genetic similarity of 0.85.The results of cluster analysis was similar with the antagonistic test.This study provided a scientific basis for strain selection in production and genetics breeding of Auricularia auricula.
参考文献/References:
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备注/Memo
第一作者简介：史灵燕（1993-），女，硕士研究生，研究方向为食用菌遗传育种。E-mail：2393355153@qq.com.责任作者：张庆桥（1966-），男，本科，副教授，现主要从事应用微生物技术等研究工作。E-mail：zhangqingqiao85@163.com.基金项目：河北省现代农业产业技术体系食用菌创新团队建设专项资金资助项目（HBCT2018050202）；阜平县太行山食用菌研究院资助项目（20170192）。收稿日期：2019-01-15