LU Sen,KANG Xingjiao,ZHAO Yufen,et al.Effect of Different Hormones on Epicotyl Germination of Oil-used Peony[J].Northern Horticulture,2019,43(02):89-94.[doi:10.11937/bfyy.20181994]
不同激素对油用牡丹上胚轴萌发的影响
- Title:
- Effect of Different Hormones on Epicotyl Germination of Oil-used Peony
- 文献标志码:
- A
- 摘要:
- 以‘凤丹白’胚根大于3 cm的种子为试验材料，采用全因子试验方法，研究了GA3、NAA和6-BA 3种激素的不同浓度和时间对胚根种子的萌芽率、发芽指数和初萌期的影响，以期为苗木生产提供依据。结果表明：GA3 500 mg·L-1处理16 h，其萌芽率、发芽指数和初萌期分别为98.89%、0.28和77.00 d，发芽指数显著高于对照、初萌期显著比对照提早了13.33 d；NAA 100 mg·L-1和 6-BA 100 mg·L-1对‘凤丹白’上胚轴萌发均没有明显的促进或抑制作用，300 mg·L-1 NAA和6-BA、500 mg·L-1 NAA和6-BA不论浸种时间长短均明显地抑制了‘凤丹白’种子的萌芽率和发芽指数；同时，500 mg·L-1 NAA显著地延长了‘凤丹白’种子的初萌期，说明500 mg·L-1 NAA严重抑制了‘凤丹白’种子的生长发育。
- Abstract:
- To improve the germination rate and germination regularity of Paeonia ostii ‘Feng Dan Bai’ and provide theoretical basis for seedling production,the effects of different densities and treated time of 3 hormones (GA3,NAA and 6-BA respectively) on germination rate,germination index and first sprouting day of seeds of Paeonia ostii ‘Feng Dan Bai’ with radicle length greater than 3 cm were studied using full factor test method.The results showed that germination rate,germination index and first sprouting day of the seeds treated for 16 hours in 500 mg·L-1 GA3 were 98.89%,0.28 and 77.00 days respectively.The germination index was significantly higher than that of control experiment,and the first sprouting day arrived 13.33 days earlier than the control.However,there were no significant effects of NAA and 6-BA at low concentration 100 mg·L-1 on the hypocotyl growth of Paeonia ostii ‘Feng Dan Bai’.The two treatments of 300 mg·L-1 NAA and 6-BA treatment,500 mg·L-1 NAA and 6-BA treatment both had significantly inhibitory effects on the germination rate and germination index of Paeonia ostii ‘Feng Dan Bai’,regardless of the duration of seed soaking.Meanwhile,the 500 mg·L-1 NAA treatment significantly extended the first sprouting day,suggesting that the growth of Paeonia ostii seeds were seriously restrained by 500 mg·L-1 NAA treatment.
相似文献/References:
[1]马玲玲,王 鹏,王淑安,等.取材时间和激素对“豫楸1号”腋芽诱导的影响[J].北方园艺,2014,38(13):84.
MA Ling-ling,WANG Peng,WANG Shu-an,et al.Effect of Sampling Time and Hormones on Axillary Buds Induction of ‘Yuqiu No.1’[J].Northern Horticulture,2014,38(02):84.
[2]鲍林文,陈哲皓,钟丹,等.不同激素组合对黄瓜“露地二号”离体子叶再生芽诱导的影响[J].北方园艺,2013,37(13):100.
BAO Lin-wen,CHEN Zhe-hao,ZHONG Dan,et al.Effects of Different Hormones on Buds Regeneration Induction from ?Cotyledons in Cucumber ‘Ludi No.2’[J].Northern Horticulture,2013,37(02):100.
[3]黄俊轩,刘艳军,李建科,等.以花椰菜花球为外植体的离体再生体系的建立[J].北方园艺,2014,38(05):88.
HUANG Jun-xuan,LIU Yan-jun,LI Jian-ke,et al.Regeneration of Cauliflower by in vitro Flower Bulb[J].Northern Horticulture,2014,38(02):88.
[4]韦善君,武运芳,杨林,等.新疆雪莲叶片高效再生植株体系的建立[J].北方园艺,2014,38(05):98.
WEI Shan-jun,WU Yun-fang,YANG Lin,et al.Establishment of High Frequency Plant Regeneration System From the Leaf of Saussurea involucrate[J].Northern Horticulture,2014,38(02):98.
[5]郭朋伟,高晔华,高日,等.“贝吉佳”草莓脱毒苗增殖的研究[J].北方园艺,2013,37(10):110.
GUO Peng-wei,GAO Ye-hua,GAO Ri,et al.Study on Proliferation of Virusfree Strawberry ‘Bechika’[J].Northern Horticulture,2013,37(02):110.
[6]高 婷.桂花嫩枝扦插繁殖技术[J].北方园艺,2013,37(18):78.
GAO Ting.Technology of Softwood Cuttings Propagation in Osmanthus fragrans (Thunb) Lour.[J].Northern Horticulture,2013,37(02):78.
[7]邵元健,周蒋陈.一品红组培苗快繁技术的研究[J].北方园艺,2012,36(02):127.
SHAO Yuan-jian,ZHOU Jiang-chen.Study on the Tissue Culture Technology of the Seedling Propagation in Euphorbia pulcherrima Wild[J].Northern Horticulture,2012,36(02):127.
[8]金亚征,姚太梅,丁丽梅,等.果树花芽分化机理研究进展[J].北方园艺,2013,37(07):193.
JIN Ya-zheng,YAO Tai-mei,DING Li-mei,et al.Research Progress on the Mechanism of Flower Bud Differentiation of Fruit Trees[J].Northern Horticulture,2013,37(02):193.
[9]高晔华,郭朋伟,高 日,等.黑果腺肋花楸组培苗生根培养及驯化的研究[J].北方园艺,2013,37(09):105.
GAO Ye-hua,GUO Peng-wei,GAO Ri,et al.Study on Rooting and Acclimatization in the Process of Tissue Culture of Aronia melanocarpa [J].Northern Horticulture,2013,37(02):105.
[10]高晔华,郭朋伟,吴荣哲.黑果腺肋花楸组培苗增殖的初步研究[J].北方园艺,2012,36(17):119.
GAO Ye-hua,GUO Peng-wei,WU Rong-zhe.Primary Study on Tissue Culture Proliferation of Aronia melanocarpa[J].Northern Horticulture,2012,36(02):119.
备注/Memo
第一作者简介：路森（1982-），男，硕士，高级工程师，现主要从事园林植物引进与栽培等研究工作。E-mail：hhzx518@163.com.责任作者：赵玉芬（1974-），女，本科，正高级工程师，现主要从事园林植物的育种与栽培和组培快繁技术等研究工作。E-mail：hbzyf74@163.com.基金项目：河北省林业科学技术研究资助项目（1501467）。收稿日期：2018-08-13