NIEJianjun,PANBaohua,LICaiping,et al.OptimizationofFormulaofExopolysaccharides FermentationMediumofLentinulaedodesA05[J].Northern Horticulture,2018,42(20):145-148.[doi:10.11937/bfyy.20180674]
香菇A05产胞外多糖发酵培养基配方优化
- Title:
- OptimizationofFormulaofExopolysaccharides FermentationMediumofLentinulaedodesA05
- Keywords:
- exopolysaccharides; fermentationmedium; formula; optimization
- 文献标志码:
- A
- 摘要:
- 以香菇A05为试材，采用单因素试验和正交实验，以胞外多糖(EPS)产量为测量指标，筛选出最佳碳氮源，并优化了香菇A05产胞外多糖发酵培养基配方。结果表明：最佳配方为玉米粉2%，葡萄糖2%，麸皮3%，牛肉浸膏0.3%，KH2PO40.1%,MgSO40.05%，pH自然，胞外多糖(EPS)产量为2.92g·L-1。
- Abstract:
- TakingLentinulaedodesA05astestmaterial，thesinglefactortestandorthogonalexperimentwereusedtomeasuretheyieldofexopolysaccharides(EPS),andtheoptimalcarbonandnitrogensourcewereselected,theformulaoffermentationmediumfortheyieldofexopolysaccharidesofLentinulaedodesA05wasoptimized.Theresultsshowedthatthebestformulawascornflour2%,glucose2%,bran3%,beefextract0.3%,KH2PO40.1%,MgSO40.05%,naturalpH,andtheyieldofexopolysaccharides(EPS)was2.92g·L-1.
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备注/Memo
第一作者简介：聂建军（1969-），男，本科，副研究员，现主要从事食(药)用菌等研究工作。E-mail：njjj666@126.com.基金项目：山西省煤基重点科技攻关资助项目(FT2014-03-11）；山西省农业科学院特色农业技术攻关资助项目(YGG17115)；山西省农业科学院山西“农谷”研发专项资助项目（YCX2018204）;山西省科技成果推广资助项目（2016CGZH28）。收稿日期：2018-04-12