YU Mei,JIAO Yingchun,SUN Xu,et al.Optimizing of Culture Medium of Agaricus bisporus (Lange) Imbach for Extracellular polysaccharide Production by Response Surface Methodology[J].Northern Horticulture,2016,40(12):152-157.[doi:10.11937/bfyy.201612038]
响应面法优化青海野生双孢菇发酵培养基
- Title:
- Optimizing of Culture Medium of Agaricus bisporus (Lange) Imbach for Extracellular polysaccharide Production by Response Surface Methodology
- Keywords:
- response surface methodology; Agaricus bisporus (Lange) Imbach; extracellular polysaccharide; culture medium; optimizing
- 文献标志码:
- A
- 摘要:
- 以野生青海双孢菇（Agaricus bisporus(Lange)Imbach）为试材，以胞外多糖含量为响应值，在单因素试验的基础上，采用4因素4水平响应面分析法，研究了培养基中不同营养因子对其菌丝代谢胞外多糖含量的影响,以确定最佳野生双孢菇发酵培养基。结果表明：最优培养基为甘露醇19.83 g?L-1，蛋白胨6.35 g?L-1，维生素B12 1.61 g?L-1，硫酸亚铁0.42 g?L-1，胞外多糖含量最高可达2.002 g?L-1，与试验模型预测值基本一致。
- Abstract:
- Taking Agaricus bisporus (Lange) Imbach as material,the content of extracellular polysaccharide as the response value,on the basis of single factor experiment,the effect of different nutritional factors on the content of extracellular polysaccharide were researched by the response surface methodology with 4 factors and 4 levels to determined the optimum culture medium.The results showed that the best culture medium for fermentation extracellular polysaccharide of the Agaricus bisporus (Lange) Imbach was mannitol 19.83 g?L-1,peptone 6.35 g?L-1,vitamin B12 1.61 g?L-1,iron sulfate 0.42 g?L-1,the highest production of extracellular polysaccharide reached 2.002 g?L-1,which was identical to the response surface prediction value.
参考文献/References:
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备注/Memo
第一作者简介：余梅（1977-），女，青海海东人，本科，工程师，研究方向为食品药品开发。E-mail:651313646@qq.com.责任作者：焦迎春(1973-)，女，河北景县人，硕士，教授，研究方向为食品发酵工程。E-mail:jyc_22@163.com.基金项目：微生物代谢国家重点实验室开放课题资助项目（MMLKF14-05）；国家自然基金资助项目（31460391）；青海省科技厅1020资助项目（2013-N-506）。