ZHANG Yan-li,GAO Yu-hong,LI Hong-lian.Primary Study on Fermentation Conditions of Antagonistic Strain S15 Against Rehmanniae Fusarium Wilt[J].Northern Horticulture,2012,36(17):127-129.
地黄枯萎病拮抗菌S15发酵条件的初步研究
- Title:
- Primary Study on Fermentation Conditions of Antagonistic Strain S15 Against Rehmanniae Fusarium Wilt
- 文章编号:
- 1001-0009(2012)17-0127-03
- 分类号:
- S 567.23+9
- 文献标志码:
- B
- 摘要:
- 从地黄田土壤中筛选到1株对地黄枯萎病有较强拮抗作用的菌株S15，并对S15进行发酵条件的初步研究。结果表明：生防菌S15发酵最合适的条件为：28℃、pH 7.5的黄豆饼粉浸出液1%，葡萄糖10 g，NaCl 2.5 g，CaCO3 2 g，蛋白胨 3 g，蒸馏水1 000 mL的培养液中以170 r/min的速度振荡培养4 d。
- Abstract:
- An actinomycete strain S15 antagonistic against Rehmanniae Fusarium Wilt was isolated from the soil in Rehmanniae field and the fermentation conditions were primary investigated.The results showed that the optimal fermentation conditions were lixivium of soybean cake 1%，glucose 10 g，NaCl 2.5 g，CaCO3 2 g，peptone 3 g，distilled water 1 000 mL，pH 7.5，temperature 28℃，170 r/min，fermenting 4 days.
参考文献/References:
[1]王天亮,张宝华,赵正伟,等.地黄枯萎病的发生及防治[J].植物保护,2002(11):39. [2]马贵龙,赫荣琳,毛永娜,等.人参锈腐病菌拮抗放线菌JY36发酵条件研究[J].吉林农业大学学报,2009,31(2):138-142. [3]周如军,傅俊范,卯婷婷,等.人参锈腐病拮抗细菌BS015最适发酵条件研究[J].吉林农业大学学报,2011,33(3):278-282. [4]方中达.植病研究方法[M].北京:农业出版社,1979. [5]郭晓芳,宗兆锋.5株生放放线菌发酵条件初探[J].西藏大学学报（自然科学版）,2010(6):112-116.
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备注/Memo
第一作者简介：张艳丽（1979-），女，河南安阳人，硕士，讲师，现主要从事植物病虫害等教学与研究工作。E-mail：katie_zyl@163.com.