ZHANG Beilin,LI Zhiliang,FANG Jiao,et al.Genetic Diversity of Rhododendron simsii Planch.Populations Sampled From Different Slope Aspects With ISSR Markers[J].Northern Horticulture,2018,42(14):97-101.[doi:10.11937/bfyy.20174748]
不同坡向映山红种群遗传多样性的ISSR研究
- Title:
- Genetic Diversity of Rhododendron simsii Planch.Populations Sampled From Different Slope Aspects With ISSR Markers
- 文献标志码:
- A
- 摘要:
- 以黄狮寨3个不同坡向的杜鹃花居群为试材，采用10个ISSR分子标记，研究了坡向对杜鹃花遗传多样性的影响。结果表明：采用的ISSR标记共扩增出38个位点，其中多态性位点36个，多态性比率94.74%，各位点扩增条带为3~5个。“南坡居群”多态性位点、Nei′s基因多样性指数（h）、Shannon信息指数（I）均最高，最低的是“西坡居群”。南坡的地理环境和伴生物种对野生杜鹃花遗传多样性影响较大。黄狮寨杜鹃花居群总的遗传变异Ht为0.268 9，仅6.01%的遗传变异发生在居群间，发生在居群内的遗传变异高达93.99%。3个居群间遗传一致度最高的居群是“西坡居群”和“北坡居群”；遗传距离最大的是“北坡居群”和“南坡居群”。为杜鹃花的生物保护、可持续开发利用等研究提供了参考依据。
- Abstract:
- In this study,ten ISSR markers were selected to investigate the genetic diversity of three Rhododendron simsii Planch.populations,which were sampled at different slope aspects of Huangshizhai (Luotian country).The results showed that these markers gave out 38 loci,among which 36 bands (94.74%) were polymorphic.Moreover,the number of locus ranged from 3 to 5.In particular,the percentage of polymorphic loci,Nei′s gene diversity (h),Shannon information index (I) were all the highest in the ‘Southern population’,while the lowest in the ‘Western population’.The geographical environment and accompanying species on the southern slope might showed great effect to genetic diversity of R.simsii population.The total genetic variation (Ht) was 0.268 9.AMOVA analysis showed that only 6.01% genetic variation was maintained between populations,while 93.99% was maintained within populations.The genetic identify was the highest between ‘Western population’ and ‘Northern population’.Furthermore,genetic distance was the highest between ‘Northern population’ and ‘Southern population’.This research would provide critical theoretical basis for the biological protection,sustainable development and utilization of Rhododendron resources.
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备注/Memo
第一作者简介：张蓓林（1993-），女，硕士研究生，研究方向为植物分子遗传学。E-mail：381355814@qq.com.责任作者：王书珍（1984-），女，博士，副教授，现主要从事植物遗传学等研究工作。E-mail：wangshuzhen710@whu.edu.cn.基金项目：经济林木种质改良与资源综合利用湖北省重点实验室和大别山特色资源开发湖北省协同创新中心联合开放基金资助项目（2017BX06）；生物学实验教学示范中心“大学生创新创业活动”类资助项目；大别山特色资源开发湖北省协同创新中心团队资助项目（2015TD07）。收稿日期：2018-03-21