[1]原晓龙,陈剑,陈中华,等.滇牡丹类黄酮5-O-葡萄糖基转移酶基因的鉴定及表达分析[J].北方园艺,2018,42(14):102-108.[doi:10.11937/bfyy.20174499]
　YUAN Xiaolong,CHEN Jian,CHEN Zhonghua,et al.Identification and Expression Analysis of a Flavonoid 5-O-glucosyltransferase Gene in Paeonia delavayi[J].Northern Horticulture,2018,42(14):102-108.[doi:10.11937/bfyy.20174499]
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滇牡丹类黄酮5-O-葡萄糖基转移酶基因的鉴定及表达分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第42卷 期数: 2018年14 页码: 102-108 栏目: 出版日期: 2018-07-25
Title:
Identification and Expression Analysis of a Flavonoid 5-O-glucosyltransferase Gene in Paeonia delavayi
作者:
原晓龙; 陈剑; 陈中华; 华梅; 王毅
（云南省林业科学院 云南省森林植物培育与开发利用重点实验室,国家林业局云南珍稀濒特森林植物保护和繁育重点实验室,云南 昆明 650201）
Author(s):
YUAN Xiaolong; CHEN Jian; CHEN Zhonghua; HUA Mei; WANG Yi
(Yunnan Provincial Key Laboratory of Cultivation and Exploition of Forest Plants,Yunnan Academy of Forestry/Key Laboratory for Conservation of Rare,Endanger & Endemic Forest Plants,State Forestry Administration,Kunming,Yunnan 650201)
关键词:
滇牡丹; 糖基转移酶; 克隆; 基因表达分析
Keywords:
Paeonia delavayi; glucosyltransferase; cloning; gene expressing analysis
DOI:
10.11937/bfyy.20174499
文献标志码:
A
摘要:
以野生黄花滇牡丹(Paeonia delavayi)为试材，采用RT-PCR技术，克隆得到一个具完整开放阅读框的类黄酮5-O-葡萄糖基转移酶([STBX]Pd5GT[STBZ])基因，并对其进行生物信息学分析和转录模式分析，以期为研究滇牡丹花色素形成机理，滇牡丹糖基转移酶功能鉴定及花卉品质改良提供理论基础。结果表明：该基因全长1 410 bp，编码469个氨基酸(GenBank登录号：ARA67364)；生物信息学分析发现Pd5GT蛋白C末端含有PSPG盒子，其氨基酸序列为WCSQVEVLSHPSLGCFVTHCGWNSTTESLVCGVPVVAFPQWTDQGTNAKL，与已知功能的5GT蛋白序列聚为一类；该基因在茎、叶中微弱表达，在花中大量表达，在不同颜色的花瓣中均可大量表达。综上所述[STBX]Pd5GT[STBZ]基因可能编码5-O-糖基转移酶，具有一定的组织特异性，花色特异性不明显。
Abstract:
Paeonia delavayi was used as experimental materials,we cloned a full length of flavonoid 5-O-glucosyltransferase([STBX]Pd5GT[STBZ]) gene from P.delavayi firstly with the method of RT-PCR,which has a whole opening reading frame,and used the bioinformatics and transcriptional pattern to analyze [STBX]Pd5GT[STBZ] gene.The results showed that the C terminal of Pd5GT protein had PSPG box,and its amino acids sequence was WCSQVEVLSHPSLGCFVTHCGWNSTTESLVCGVPVVAF PQWTDQGT NAKL,and clustered together with 5GT known their function;[STBX]Pd5GT[STBZ] gene was strongly expressed in flower,slightly in stem and leaves,and strongly expressed in different color petals.In conclusion,[STBX]Pd5GT[STBZ] gene could encode 5-O-glucosyltransferase,had some tissue-speciality but flower-color-speciality wasn′t obviously.The present study would provide theoretical basis for the study of mechanism of flower pigment biosynthesis in P.delavayi,for the identification of the function of glucosyltransferase in P.delavayi,and for improving flower quality by genetic engineering.

相似文献/References:

[1]原晓龙,李子光,陆刚,等.滇牡丹查尔酮异构酶基因的克隆及表达分析[J].北方园艺,2020,44(22):79.[doi:10.11937/bfyy.20194324]
　YUAN Xiaolong,LI Ziguang,LU Gang,et al.Cloning and Expression Analysis of CHI of Paeonia delavayi[J].Northern Horticulture,2020,44(14):79.[doi:10.11937/bfyy.20194324]
[2]刘子榕,平怀磊,蒲艳,等.植物生长调节剂对不同基质滇牡丹嫩硬枝扦插的效果[J].北方园艺,2022,(23):74.[doi:10.11937/bfyy.20221491]
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[3]杜春,平怀磊,王文治,等.滇牡丹PdANR基因克隆及表达模式分析[J].北方园艺,2022,(24):42.[doi:10.11937/bfyy.20221618]
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备注/Memo

第一作者简介：原晓龙(1986-)，男，陕西蒲城人，硕士，助理研究员，现主要从事分子生物学等研究工作。E-mail：xiaolony@126.com.责任作者：王毅(1981-)，男，四川广安人，博士，副研究员，现主要从事植物学和分子生物学等研究工作。E-mail：22825818@qq.com.基金项目：云南省应用基础研究青年资助项目(2016FD100);云南省林业科学院创新基金资助项目（QN2018-01）。收稿日期：2018-03-01

更新日期/Last Update: 2018-08-20