[1]陈春香,王飞,李文斌,等.夏天无染色体加倍技术研究[J].北方园艺,2017,41(18):157-160.[doi:10.11937/bfyy.20165112]
　CHEN Chunxiang,WANG Fei,LI Wenbin,et al.Study on Chromosome Doubling in Corydalis decumbens[J].Northern Horticulture,2017,41(18):157-160.[doi:10.11937/bfyy.20165112]
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夏天无染色体加倍技术研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第41卷 期数: 2017年18 页码: 157-160 栏目: 出版日期: 2017-09-30
Title:
Study on Chromosome Doubling in Corydalis decumbens
作者:
陈春香1; 王飞1; 李文斌2; 余志光2; 曾建华1; 黄胜和1
1.南昌大学 抚州医学院，江西 抚州 344000；2.江西天施康中药股份有限公司，江西 鹰潭 335000
Author(s):
CHEN Chunxiang1; WANG Fei1; LI Wenbin2; YU Zhiguang2; ZENG Jianhua1; HUANG Shenghe1
1.Fuzhou Medical College,Nanchang University,Fuzhou，Jiangxi 344000；2.Jiangxi Herbi-sky Chinese Medicine Co.Ltd.,Yingtan，Jiangxi 335000
关键词:
夏天无; 秋水仙素; 染色体加倍
Keywords:
Corydalis decumbens (Thunb.) Pers.; colchicine; chromosome doubling
DOI:
10.11937/bfyy.20165112
文献标志码:
A
摘要:
以夏天无块茎为试材，采用不同浓度秋水仙素（0.00%、0.05%、0.10%、0.20%）添加2%二甲基亚砜(DMSO)分别浸泡24、48、72 h的方法，研究了不同浓度秋水仙素和不同处理时间对染色体加倍的影响。结果表明：以0.20%秋水仙素处理24 h较优，成活率为31.4%，加倍率为50.0%，其次为0.10%秋水仙素处理24、48 h和0.20%秋水仙素处理48 h。其中，未加倍的植株染色体数为2n=28，加倍后的植株为2n=56。
Abstract:
Tubers from Corydalis decumbens (Thunb.) Pers.were immersed in 0.00%,0.05%，0.10% and 0.20% colchicine solution with 2% DMSO for 24 hours，48 hours，72 hours,respectively.The effects of different concentrations of colchicine and different treatment time on chromosome doubling were studied.The results indicated that the better treatment was 0.20% colchicine solution for 24 hours with a doubling rate of 50.0% and a survival rate of 31.4%.Secondly for 0.10% colchicine solution for 24 hours，48 hours and 0.20% colchicine solution for 48 hours.The chromosome number of diploid Corydalis decumbens (Thunb.) Pers.was 2n=28 and that of tetraploid was 2n=56.

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备注/Memo

第一作者简介：陈春香（1981-），女，硕士，讲师，研究方向为生物化学与分子生物学。E-mail：ccx99440@163.com.

责任作者：黄胜和（1977-），男，博士，副教授，研究方向为药用植物生物技术。E-mail：hsh712@163.com.
基金项目：江西省科技支撑计划资助项目（20133BBF60078）。
收稿日期：2017-02-03

更新日期/Last Update: 2017-10-07