YANG Wenting,KUANG Qian.Study on the Anti-browning Method in Tissue Culture of Taxus chinensis[J].Northern Horticulture,2016,40(17):111-114.[doi:10.11937/bfyy.201617026]
红豆杉组织培养的防褐变措施研究
- Title:
- Study on the Anti-browning Method in Tissue Culture of Taxus chinensis
- Keywords:
- Taxus chinensis; callus; induction rate of callus; browning
- 文献标志码:
- A
- 摘要:
- 以红豆杉腋芽为外植体，B5为基本培养基，研究了不同激素、添加剂组合对红豆杉组织培养中褐变的影响。结果表明：添加激素2,4-D 2.0 mg?L-1+6-BA 0.5 mg?L-1，愈伤组织生长情况最好，染菌率为11.11%，愈伤组织出愈率最高达88.89%，褐化程度较低，多酚氧化酶（PPO）活性为8.03 U?g-1?min-1；加入添加剂组合活性炭和柠檬酸，可以降低红豆杉的褐变程度，PPO活性为1.40 U?g-1?min-1。
- Abstract:
- With the yew axillary buds as explants，B5 as basic culture medium，the effect of different combinations of hormone and additives on browning rate in tissue culture of Taxus was studied.The results showed that under the hormone concentration of 2,4-D 2.0 mg?L-1 and 6-BA 0.5 mg?L-1，the callus grown well，whose contamination rate was 11.11%，the induction rate of callus was 88.89%，browning degree was lower，and PPO was 8.03 U?g-1?min-1.Furthermore，by adding the combination of activated carbon and citric acid，the degree of browning reduced significantly，and PPO was 1.40 U?g-1?min-1.
参考文献/References:
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备注/Memo
第一作者简介：杨文婷（1983-），女，硕士，讲师，现主要从事药用资源的开发利用等研究工作。E-mail：47389578@qq.com.基金项目：湖北省教育厅科学技术研究资助项目（B2016337）；武汉工商学院校级学术团队“特色生物资源开发与利用”基金资助项目（XSTD2015003）。