GUO Chunmei,HE Junping,TU Xiaoyun,et al.Study on the Rooting Medium of Space Phalaenopsis[J].Northern Horticulture,2016,40(02):103-104.[doi:10.11937/bfyy.201602027]
太空蝴蝶兰壮苗生根培养基的研究
- Title:
- Study on the Rooting Medium of Space Phalaenopsis
- Keywords:
- space Phalaenopsis; medium; auxin; additives
- 文献标志码:
- A
- 摘要:
- 以太空蝴蝶兰种子为试材，研究了不同培养基、不同NAA浓度及有机添加物对太空蝴蝶兰壮苗生根的影响。结果表明：基本培养基以1/2MS培养基最为适宜，生根率达98.9%，苗株较强壮。在1/2MS培养基中加入1.0 mg/L NAA时生根效果较好，生根率达100%，且苗株强壮。香蕉泥和椰子汁对太空蝴蝶兰生根及生长具有明显促进作用。因此，确定太空蝴蝶兰最佳壮苗生根培养基为：1/2MS+NAA 1.0 mg/L+香蕉泥30 g/L+椰子汁100 mL/L+蔗糖20 g/L+活性炭1 g/L+琼脂6 g/L。
- Abstract:
- Using the seed of space Phalaenopsis as explants，the effect of different medium，NAA conentrations and additives on the seeding roots of space Phalaenopsis was studied.The results showed that the 1/2MS was the appropriate medium，the rooting rate was 98.9%；adding 1.0 mg/L NAA，the rooting rate was 100% and the recruits grew stronger；banana and coconut milk had significant promotion to rooting growth of space Phalaenopsis.Therefore，the best rooting medium was 1/2MS+NAA 1.0 mg/L+banana 30 g/L+coconut milk 100 mL/L+sucrose 20 g/L+activated carbon 1 g/L+agar 6 g/L.
参考文献/References:
[1]杨万立，齐丽省.太空育种[J].生物学教学，2005（3）：67-68. [2]陈燕，徐墨涵，尹静.太空育种[J].生物学教学，2008（10）：67-69. [3]潘虹虹，孙丹，廉美兰，等.几种外部因子对春石斛组培生根的影响[J].安徽农业科学，2008,36（29）：12591-12594. [4]张敏，黄利斌，蒋泽平.蝴蝶兰组织培养生根影响因子的筛选[J].江苏林业科技，2011，38（1）：20-22. [5]何松林，王献，鲁琳，等.培养基和添加物对蝴蝶兰原球茎分化幼苗的影响[J].中南林学院学报，2003，23(5)：11-13. [6]何松林，孔德政，杨秋生，等.碳源和有机添加物对文心兰圆球茎增殖的影响[J].河南农业大学学报，2003，37(2)：154.
相似文献/References:
[1]于海燕,陈刚,建德锋,等.毛花猕猴桃组培苗增殖扩繁培养基筛选试验[J].北方园艺,2013,37(04):111.
[2]马紫英,夏 斌,倪 焱,等.以椰子壳为主碳源的巨大口蘑原种培养基优化[J].北方园艺,2014,38(13):142.
MA Zi-ying,XIA Bin,NI Yan,et al.Optimization of Culture Medium for Second-Class Spawn of Tricholoma giganteum Using Coconut Shell as Main Carbon Source[J].Northern Horticulture,2014,38(02):142.
[3]郑 晟,邓 赞,张凌云.大白杜鹃种子无菌萌发及幼苗生长条件研究[J].北方园艺,2014,38(11):77.
ZHENG Sheng,DENG Zan,ZHANG Ling-yun.Research on Seeds Aseptic Germination and Seedling Growth Condition of Rhododendron decorum Franch[J].Northern Horticulture,2014,38(02):77.
[4]朱会霞.灵芝真菌发酵产灵芝酸培养基优化研究[J].北方园艺,2013,37(22):154.
ZHU Hui-xia.Study on the Optimization of Culture Medium of Ganoderma Fermentation Before Extraction of Ganoderic Acid[J].Northern Horticulture,2013,37(02):154.
[5]唐业刚,崔 伟.宜昌百合鳞茎增殖诱导条件研究[J].北方园艺,2014,38(02):112.
TANG Ye-gang,CUI Wei.Research on Proliferation Culture Condition of Lilium leucanthum Baker Bulb[J].Northern Horticulture,2014,38(02):112.
[6]郭欣欣,樊淑华,王永立.金针菇液体培养基的优化研究[J].北方园艺,2014,38(02):146.
GUO Xin-xin,FAN Shu-hua,WANG Yong-li.Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes [J].Northern Horticulture,2014,38(02):146.
[7]郭朋伟,高晔华,高日,等.“贝吉佳”草莓脱毒苗增殖的研究[J].北方园艺,2013,37(10):110.
GUO Peng-wei,GAO Ye-hua,GAO Ri,et al.Study on Proliferation of Virusfree Strawberry ‘Bechika’[J].Northern Horticulture,2013,37(02):110.
[8]周慧杰.灰树花的母种及原种培养基筛选研究[J].北方园艺,2013,37(14):155.
ZHOU Hui-jie.Study on Selection of Mother and Original Culture Media of Grifora frondosa[J].Northern Horticulture,2013,37(02):155.
[9]杨丽维,王玉,班立桐,等.杏鲍菇液体菌种培养基的筛选和优化[J].北方园艺,2014,38(06):150.
YANG Li-wei,WANG Yu,BAN Li-tong,et al.Study on the Screening and Optimizing of Liquid Spawn Medium of Pleurotus eryngii[J].Northern Horticulture,2014,38(02):150.
[10]忻龙祚,金亚征,苗文静.蒙古口蘑菌丝的室内优化培养研究[J].北方园艺,2013,37(11):140.
XIN Long-zuo,JIN Ya-zheng,MIAO Wen-jing.Study on Optimization of Mycelia Culture of Mongolia Mushroom from Indoor[J].Northern Horticulture,2013,37(02):140.
备注/Memo
第一作者简介：郭春梅（1970-），女，江苏连云港人，本科，工程师，现主要从事观赏植物生物技术研究和生产技术推广等工作。E-mail：297098635@qq.com. 基金项目：国家花卉产业技术创新战略联盟资助项目（2014hhlm010）。