CAO Huiying,ZHANG Lijun,LUO Zhen,et al.Change of Trans-zeatin in Tomato Regeneration in vivo[J].Northern Horticulture,2015,39(24):66-69.[doi:10.11937/bfyy.201524020]
番茄活体再生中反式玉米素含量的变化
- Title:
- Change of Trans-zeatin in Tomato Regeneration in vivo
- Keywords:
- cytokinin; HPLC; regeneration mechanism; tomato
- 文献标志码:
- A
- 摘要:
- 以番茄活体植株为试材，采用高效液相色谱（HPLC）法研究了番茄活体再生中反式玉米素含量的变化。结果表明：在愈伤组织的形成过程中，反式玉米素逐渐增加，并且在愈伤组织最发达时达到峰值。随着愈伤组织分化，其含量略有降低。此变化规律与番茄离体再生中外源反式玉米素使用浓度由高到低的变化规律基本吻合。活体再生中，用细胞分裂素合成抑制剂洛伐他丁处理伤口不影响再生进程。
- Abstract:
- Taking tomato plant as material，change of trans-zeatin in vivo was researched by high performance liquid chromatography (HPLC).The results showed that the content of trans-zeatin increased gradually with the formation of callus，and became peak in well-developed ones.When callus differentiated，the content decreased lightly.The content of trans-zeatin variation of regeneration in vivo was identical to in vitro.Lovastatin which could suppress the biosynthesis of cytokinins smeared in the cut surfaces could not impress the regeneration of tomato in vivo.
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备注/Memo
第一作者简介：曹慧颖（1975-），女，博士，讲师，现主要从事植物分子生物学等研究工作。E-mail:497110804@qq.com.责任作者：夏润玺（1972-），男，博士，讲师，现主要从事分子生物学等研究工作。E-mail:xiarunxi@126.com.基金项目：国家自然科学基金资助项目(31300164)；教育部博士学科点专项科研基金资助项目（20122103120004）。