LI Min,XI Guang-sheng,LUO Yi-yuan,et al.Study on the Antioxidant Activity of Polysaccharides and Flavonoids in Different Strains of Polygonatum odoratum[J].Northern Horticulture,2015,39(05):135-138.[doi:10.11937/bfyy.201505039]
不同品系玉竹多糖及黄酮抗氧化活性研究
- Title:
- Study on the Antioxidant Activity of Polysaccharides and Flavonoids in Different Strains of Polygonatum odoratum
- Keywords:
- Polygonatum odoratum; polysaccharide; flavonoids; antioxidant activities; different strains
- 文献标志码:
- A
- 摘要:
- 以不同品系、不同生长年限玉竹为试材,以维生素C、芦丁作为参照,利用DPPH比色法和总还原能力测定法,以IC50作为判定抗氧化活性强弱的综合指标,对不同品系玉竹多糖及总黄酮体外抗氧化能力进行比较研究。结果表明:不同品系玉竹中二年生大玉竹多糖的IC50为0.07 mg/mL(维生素C为0.05 mg/mL)、黄酮IC50为0.048 mg/mL(芦丁为0.0062 mg/mL)及最大还原能力数值为1.698,与标准对照品相差最小,效果最好。研究结果表明二年生大玉竹抗氧化性最好。
- Abstract:
- With different strain and different age of Polygonatum ocloratum as test materials,in vitro antioxidant activities of polysaccharides and flavones from different strains of Polygonatum odoratum were investigated by employing both DPPH scavenging assay recovery ability with vitamin C as control.Using IC50 as a comprehensive index to compare of polysaccharides and flavonoids antioxidant capacity in vitro for different strains of Polygonatum odoratum.The results showed that IC50 value of biennia Polygonatum odoratum polysaccharide from different strains was 0.07 mg/mL (vitamin C was 0.05 mg/mL),IC50 value of flavonoids was 0.048 mg/mL (rutin was 0.0062 mg/mL) and the maximum reduction capacity was 1.698,and biennial Polygonatum odoratum had the minimum difference compared with control materials.The antioxidant activities of biennial Polygonatum odoratum was the best.
参考文献/References:
[1] 韩鹏然,白明,苗明三.中华人民共和国药典[J].2010年版.中医学报,2013(6):863-864. [2] 秦海林,李志宏,王鹏,等.中药玉竹中新的次生代谢产物[J].中国中药杂志,2004(1):46-48. [3] 吴晓岚,王玉勤,车光昇,等.黄精和玉竹抗疲劳作用的实验研究[J].中国冶金工业医学杂志,2009(3):271-272. [4] 梁海霞,李焕德.玉竹的药理活性研究进展[J].中南药学,2008(3):342-344. [5] 许丽丽,展晓日,曾昭武,等.玉竹多糖的研究进展[J].中药材,2011(1):154-157. [6] 宁慧,李会宁,杨培君.玉竹多糖的抗氧化作用研究[J].陕西理工学院学报(自然科学版),2013(6):59-65. [7] 徐大量,林晖.玉竹水提取液体外抗氧化得实验研究[J].中药材,2008(5):729-731. [8] 钟方丽,王晓林,杨云涛,等.超声波辅助法提取玉竹中总黄酮[J].吉林化工学院学报,2012(1):22-25. [9] 张轩铭,王冬梅,王瑾,等.不同产地玉竹黄酮提取物体外抗氧化活性研究[J].西北植物学报,2011(3):628-631. [10]阎欲晓,石庆师.玉竹多糖分离纯化及自由基清除能力研究[J].食品工业科技,2009(2):149-151.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]谭启明,付瑞敏,李景原,等.响应面法优化微波辅助提取芦荟凝胶多糖工艺[J].北方园艺,2014,38(13):133.
TAN Qi-ming,FU Rui-min,LI Jing-yuan,et al.Optimization of Microwave-assisted Extraction Process of Aloe barbadensis Miller Polysaccharies by Response Surface Method[J].Northern Horticulture,2014,38(05):133.
[3]徐 雷,刘常丽,张 群,等.不同初加工方法对茯苓多糖和三萜类成分的影响[J].北方园艺,2014,38(10):148.
XU Lei,LIU Chang-li,ZHANG Qun,et al.Effect of Different Processing Methods on Polysaccharides and Triterpenes of?Poria [J].Northern Horticulture,2014,38(05):148.
[4]罗 琴,林惠昆,管春平,等.正交实验法优化提取二型鳞毛蕨多糖工艺的研究[J].北方园艺,2013,37(22):146.
LUO Qin,LIN Hui-kun,GUAN Chun-ping,et al.Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment[J].Northern Horticulture,2013,37(05):146.
[5]陈玉琴,南海娟,刘坤峰.蕨菜多糖提取及抗氧化特性[J].北方园艺,2014,38(14):128.
CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(05):128.
[6]魏楠楠,姚 睿,李 阳,等.几种因素对反应器铁皮石斛原球茎生长及有效物质的影响[J].北方园艺,2014,38(14):156.
WEI Nan-nan,YAO Rui,LI Yang,et al.Effect of Several Factors on Accumulation of Biomass and Bioactive Compounds during Bioreactor Culture of Dendrobium candidum PLB[J].Northern Horticulture,2014,38(05):156.
[7]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(05):148.
[8]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(05):154.
[9]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(05):166.
[10]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(05):135.
[11]马 尧,刘 野.不同移栽密度对玉竹有效成分的影响[J].北方园艺,2015,39(03):137.[doi:10.11937/bfyy.201503040]
MA Yao,LIU Ye.Effect of Different Transplanting Density on the Effective Constituents of Polygonatum odoratum[J].Northern Horticulture,2015,39(05):137.[doi:10.11937/bfyy.201503040]
备注/Memo
第一作者简介:李敏(1984-),女,硕士,讲师,研究方向为天然药物化学。E-mail:limin1042004@163.com. 责任作者:奚广生(1967-),男,硕士,教授,现主要从事药用植物品种选育及成分提取等研究工作。E-mail:zyxyxgs@126.com. 基金项目:吉林农业科技学院校重点学科培育资助项目(吉农院合字2013第S012号)。