LUO Qin,LIN Hui-kun,GUAN Chun-ping,et al.Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment[J].Northern Horticulture,2013,37(22):146-148.
正交实验法优化提取二型鳞毛蕨多糖工艺的研究
- Title:
- Study on the Optimization of Extraction Technology of Polysaccharide from Dryopteris cochleata Using Orthogonal Experiment
- 文章编号:
- 1001-0009(2013)22-0146-03
- Keywords:
- Dryopteris cochleata; polysaccharide; extraction; orthogonal test
- 分类号:
- R 284.1
- 文献标志码:
- A
- 摘要:
- 以二型鳞毛蕨为试材，通过单因素试验研究了浸提温度、料液比、提取时间和乙醇浓度对多糖提取率的影响，并采用正交实验优化了二型鳞毛蕨多糖提取工艺。结果表明：多糖最佳提取工艺为浸提时间2.5 h，料液比1∶40 g/mL，乙醇浓度80%，浸提温度80℃，在此条件下，多糖的提取率为2.60％;经该试验优化后的工艺合理，多糖提取率高，品质较好。
- Abstract:
- Taking Dryopteris cochleata as material,the effect of extraction temperature,solid-liquid ratio,extraction time and the ethanol concentration on polysaccharide yield were studied by single factor test,and the extraction technology was optimized by orthogonal experiment.The results showed that the best extraction technology of polysaccharide from Dryopteris cochleata was the extraction time 2.5 h,the solid-liquidratio 1∶40 g/mL,the ethanol concentration 80%and extraction temperature 80℃.Under these conditions,the extraction rate of polysaccharide was 2.60%.The optimized extraction process was reliable,and the extraction efficiency of polysaccharide was higher than normal with good quality.
参考文献/References:
[1] 武素功.中国植物志[M].北京:科学出版社,2000.
[2] 徐成东.云南哀牢山国家级自然保护区蕨类植物资源[J].楚雄师范学院学报,2006,21(9):51-58.
[3] 谢宗万,余友芩.全国中草药名鉴[M].北京:人民卫生出版社,1996.
[4] 陈旋,张翼,张剑波.植物多糖的研究进展[J].中国新药杂志,2007,16(13):1000-1005.
[5] 王玲,和兆荣.药用蕨类植物化学成分研究进展[J].中国野生植物资源,2006,25(3):1-4.
[6] 赵莉,杨文钰.蕨类植物的活性成分研究进展[J].中药材,2004,27(6):452-456.
[7] Jie L R.Orthogonal test design for optimization of the extraction of polysaccharides from Phascolosoma esulenta and evaluation of its immunity activity[J].Carbohydrate Polymers,2008,73:558-560.
[8] 秦向东,宁玲,闫小颜.棒节石斛中的多糖分布及提取工艺研究[J].云南农业大学学报,2011,26(3):430-433.
[9] 甘璐,张声华.不同品种枸杞多糖研究[J].食品科学,1996,17(9):9-12.
相似文献/References:
[1]李海平,何玥,闫军,等.响应面法优化超声提取地皮菜多糖工艺研究[J].北方园艺,2013,37(04):138.
[2]谭启明,付瑞敏,李景原,等.响应面法优化微波辅助提取芦荟凝胶多糖工艺[J].北方园艺,2014,38(13):133.
TAN Qi-ming,FU Rui-min,LI Jing-yuan,et al.Optimization of Microwave-assisted Extraction Process of Aloe barbadensis Miller Polysaccharies by Response Surface Method[J].Northern Horticulture,2014,38(22):133.
[3]徐 雷,刘常丽,张 群,等.不同初加工方法对茯苓多糖和三萜类成分的影响[J].北方园艺,2014,38(10):148.
XU Lei,LIU Chang-li,ZHANG Qun,et al.Effect of Different Processing Methods on Polysaccharides and Triterpenes of?Poria [J].Northern Horticulture,2014,38(22):148.
[4]陈玉琴,南海娟,刘坤峰.蕨菜多糖提取及抗氧化特性[J].北方园艺,2014,38(14):128.
CHEN Yu-qin,NAN Hai-juan,LIU Kun-feng.Extraction Technology and Antioxidant Acitvity of Polysaccharide From Pteridium revolutum [J].Northern Horticulture,2014,38(22):128.
[5]魏楠楠,姚 睿,李 阳,等.几种因素对反应器铁皮石斛原球茎生长及有效物质的影响[J].北方园艺,2014,38(14):156.
WEI Nan-nan,YAO Rui,LI Yang,et al.Effect of Several Factors on Accumulation of Biomass and Bioactive Compounds during Bioreactor Culture of Dendrobium candidum PLB[J].Northern Horticulture,2014,38(22):156.
[6]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(22):148.
[7]韩凤波,奚广生.不同采收期对轮叶党参有效成分含量的影响[J].北方园艺,2014,38(01):154.
HAN Feng-bo,XI Guang-sheng.Effect of Different Harvest Time on the Effective Component Content of Codonopsis lanceolata [J].Northern Horticulture,2014,38(22):154.
[8]姜少娟.毛竹叶多糖超声提取工艺研究[J].北方园艺,2013,37(13):166.
JIANG Shao-juan.Research on Ultrasonic Wave Extraction of Polysaccharides from Phyllostachys pubescens Leaves[J].Northern Horticulture,2013,37(22):166.
[9]胡月芳.淮山多糖微波法提取及清除羟基自由基的研究[J].北方园艺,2014,38(02):135.
HU Yue-fang.Study on the Microwave Extraction and Hydroxy Radical Scavenging of Yam Polysaccharide[J].Northern Horticulture,2014,38(22):135.
[10]谢银军,吴香梅,张培旗.超高压提取鱼腥草多糖最佳工艺研究[J].北方园艺,2013,37(10):153.
XIE Yin-jun,WU Xiang-mei,ZHANG Pei-qi.Study on Extraction Process Optimum of Polysaccharides from Houttuynia cordata Thunb Using Ultra High Pressure Method[J].Northern Horticulture,2013,37(22):153.
备注/Memo
第一作者简介：罗琴（1963-），女，云南姚安人，本科，副教授，研究方向为分子光谱及天然有机物。E-mail:hxxlqq@163.com.
责任作者：谢启明（1961-），男，云南玉溪人，本科，教授，研究方向为分子光谱及天然有机物。E-mail:hxxxqm@163.com.
基金项目：国家自然科学基金资助项目（31260095）；
云南省应用基础研究计划资助项目（200667）。
收稿日期：2013-06-19