WANG Xiao-jie,SHENG Dong-feng.Effect of Different Solvents on Extraction of Effective Total Flavones and Antibacterial Activity of Ligustrum lucidum [J].Northern Horticulture,2014,38(09):140-147.
不同溶剂对女贞子黄酮提取效果和抑菌活性的影响
- Title:
- Effect of Different Solvents on Extraction of Effective Total Flavones and Antibacterial Activity of Ligustrum lucidum
- 文章编号:
- 1001-0009(2014)09-0137-03
- 分类号:
- R 282.71
- 文献标志码:
- B
- 摘要:
- 以女贞子为试材，以75%甲醇、75%乙醇、75%丙酮、蒸馏水作为溶剂，利用分光光度法测定了不同溶剂浸提女贞子总黄酮的含量，并测定了各样品提取液对金黄色葡萄球菌、枯草杆菌、大肠杆菌、根霉、曲霉的抑菌效果，研究不同溶剂对女贞子黄酮提取效果和抑菌活性的影响。结果表明：75%甲醇对总黄酮的提取率较高，为0.347 mg/g；女贞子的乙醇提取液对细菌具有较高的抑菌作用。
- Abstract:
- Taking Ligustrum lucidum as material,the effect of different solvents on extraction of effective total flavones and antibacterial activity of Ligustrum lucidum were studied.The content of total flavones in Ligustrum lucidum was measured using the spectrophotometric method，and 75% methanol，75% ethanol，75% acetone and distilled water were chosen as the extraction solvents.The bacteriostasis activities of them were also compared by measuring the bacteriostasis activities of Staphyloccocus aureus，Hay bacillus，Escherichia coli,Rhizopus chinehsis and Aspergillus of the sample extracts.The results showed that methanol extract had the highest extraction yield of total flavones，the content was 0.347 mg/g;the ethanol extract of Ligustrum lucidum had higher bacteriostasis activity on bacteria than fungi.
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备注/Memo
第一作者简介：王晓洁（1990-），女，河南濮阳人，本科，研究方向为饲料资源开发与利用。