YANG Yan-jun,WANG Ya-hong,WANG Jun-long.Study on the Purification Process of Total Flavonoids of Sedum aizoon L. by Macroporous Adsorption Resin[J].Northern Horticulture,2014,38(07):144-147.
景天三七总黄酮大孔吸附树脂纯化工艺研究
- Title:
- Study on the Purification Process of Total Flavonoids of Sedum aizoon L. by Macroporous Adsorption Resin
- 文章编号:
- 1001-0009(2014)07-0144-04
- 分类号:
- R 284.1
- 文献标志码:
- A
- 摘要:
- 以景天三七为试材，研究了AB-8、HPD-100、D-101、DM-301、DM130 5种吸附树脂对景天三七总黄酮的静态吸附及解吸性能，并以景天三七总黄酮纯度为指标，通过单因素试验考察其吸附和洗脱条件，研究DM-301大孔吸附树脂分离纯化景天三七总黄酮的最优工艺条件。结果表明：优选的工艺条件为上样液pH为3.0，质量浓度约为1.63 mg/mL，吸附速率2 BV/h，用70%乙醇溶液以2 BV/h解吸速率解吸，解吸液体积为5 BV时解吸较完全，纯化后干浸膏中总黄酮由原来的8.99%提高到25.60%，树脂富集倍数约为3倍；DM-301型大孔树脂用于富集景天三七总黄酮效果较好,优选的工艺操作简单、方法可靠。
- Abstract:
- Taking Sedum aizoon L.as material,the dynamic adsorption and desorption performances of five kinds of macroporous adsorption resin including AB-8,HPD-100,D-101,DM-301,DM-130 were researched to choose resin type；With the concentration of total flavonoids as indexes,adsorption and desorption conditions were investigated by single factor experiments.The technological conditions of the purification process of total flavonoids from Sedum aizoon L.by macroporous adsorption resin was investigated.The results showed that DM-301 resin was selected to optimum purification technology,the conditions were：sample solution pH value was 3.0,the concentration was 1.63 mg/mL,the adsorption rate was 2 BV/h，2 BV/h desorption rate using was 70% ethanol solution,desorption solution was 5 BV.The total flavonoids of dry extract was purified from 8.99% to 25.6%,the enrichment ratio was about 3 times.DM-301 macroporous resin used for enriching the total flavonoids of Sedum aizoon L.was better,the optimized operation was simple,the method was reliable.
参考文献/References:
[1]刘晔峰,马峥嵘,吴晓琴,等.养心草的有效成分及功能性化合物研究进展[J].中华中医药杂志,2012,27(8)：2135-2137. [2]车苏容,郭素华,竺叶青.景天三七的药学研究进展[J].福建中医药,2004,35(3)：51-53. [3]凌桂梅,黄子冬,时宗泽.土三七的现代研究进展[J].浙江中医药大学学报,2012,36(3)：353-355. [4]林珠灿,黄华花,陈达炜,等.养心草宁心安神活性部位的化学成分研究[J].中华中医药杂志,2011,12(26)：3000-3002. [5]车苏容,郭素华,竺叶青.养心草的生药学研究[J].福建中医学院学报,2005,15(5)：27-30. [6]付煜荣,陈桂敏.景天三七中总酚酸提取条件及含量分析研究[J].河北北方学院学报(自然科学版),2005,21(6)：25-27. [7]陈建琴,许雪琴.养心草中齐墩果酸的含量测定[J].莆田学院学报,2009,16(2)：46-48. [8]于志斌,杨广义,吴霞,等.救心草的化学成分研究 [J].天然产物研究与开发,2007（19）：67-69. [9]郭素华,林珠灿,张志炜.养心草中山奈酚成分的HPLC-MS/MS分析及含量测定[J].中华中医药杂志,2007,22(6)：360-363. [10]李卫林,荆云,罗秋燕,等.景天三七的化学成分研究[J].新乡医学院学报,2008,25(6)：558-561. [11]王二丽,郭素华,蔡扬帆.养心草的药用价值及开发前景[J].海峡药学,2010,22(9)：79-81. [12]秦爱英.美蔬新秀-养心草[J].蔬菜,2006(8)：13-14. [13]王小会.景天三七叶茶及其饮料的加工技术研究[D].泰安：山东农业大学,2012：1-51. [14]郭芳彬.蜂产品中黄酮类化合物的保健作用[J].养蜂科技,2005(2)：28-31. [15]李园园,王俊霞,曹乃峰,等.河南产景天三七抗氧化活性研究[J].天然产物研究与开发,2011,23(2)：337-340. [16]李加林,吴素珍,卑占宇.盐肤木总黄酮提取工艺研究[J].时珍国医国药,2009,20(5)：1117.
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备注/Memo
第一作者简介：杨艳俊（1974-），女，硕士，助教，现主要从事天然产物有效成分的提取和精制。E-mail:532101728@qq.com.