[1]练华山,杨贵先,李焕秀.不同氮源对铁皮石斛原球茎液体悬浮培养的影响[J].北方园艺,2014,38(07):152-155.
　LIAN Hua-shan,YANG Gui-xian,LI Huan-xiu.Effect of Different Nitrogen Sources on Dendrobium candicum Wall.ex Lindl Suspension Cultures[J].Northern Horticulture,2014,38(07):152-155.
点击复制

不同氮源对铁皮石斛原球茎液体悬浮培养的影响

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年07期 页码: 152-155 栏目: 出版日期: 2014-04-15
Title:
Effect of Different Nitrogen Sources on Dendrobium candicum Wall.ex Lindl Suspension Cultures
文章编号:
1001-0009(2014)07-0152-04
作者:
练华山1; 2; 杨贵先2; 3; 李焕秀2
1.成都农业科技职业学院，四川 成都 610000；
2.四川农业大学 园艺学院，四川 雅安 625014；
3.东北农业大学 园艺学院，黑龙江 哈尔滨 150030
Author(s):
LIAN Hua-shan1; 2; YANG Gui-xian2; 3; LI Huan-xiu2
1.Chengdu Vocational College of Agricultural Science and Technology,Chengdu,Sichuan 610000；
2.College of Horticulture，Sichuan Agricultural University,Ya’an,Sichuan 625014；
3.College of Horticulture,Northeast Agricultural University,Harbin,Heilongjiang 150030
关键词:
铁皮石斛; 氮源; 增殖; 多糖; 蛋白质
Keywords:
Dendrobium candicum; nitrogen; proliferation; polysaccharides; protein
分类号:
R 931.71
文献标志码:
A
摘要:
以铁皮石斛为试材，以1/2MS培养基为基本培养基，添加不同种类的附加氮源20 mmol/L，研究了不同氮源对铁皮石斛原球茎悬浮培养增殖、多糖含量及蛋白质含量的影响。结果表明：最有利于铁皮石斛原球茎悬浮培养增殖的是甘氨酸，硝态氮次之，谷氨酸最差，甚至出现了负增长；在多糖积累方面，硝酸钙对铁皮石斛原球茎多糖积累最为明显，赖氨酸等不利于铁皮石斛原球茎的多糖积累；硝酸钾、硫酸铵有利于铁皮石斛原球茎蛋白质的积累，尿素等其它氮源则不利于铁皮石斛原球茎蛋白质的积累。综合分析发现，硝酸钙最利于铁皮石斛原球茎悬浮培养生长。
Abstract:
Taking Dendrobium candicum as material,with 1/2 MS as the basic medium,20 mmol/L different types of additional nitrogen sources were used,the effect of different nitrogen sources on Dendrobium candicum suspension culture proliferation,polysaccharides content and protein content were studied.The results showed that most conducive Dendrobium candicum suspension culture proliferation was glycine,followed by nitrate,and the worst was glutamate.In the accumulation of polysaccharide,the most obvious of polysaccharides accumulate was Ca(NO3)2.On the contrary,lysine was not conducive to polysaccharides accumulation for Dendrobium candicum.KNO3 and (NH4)2SO4 were conducive Dendrobium candicum accumulation of protein,urea and other nitrogen were not conducive to the accumulation of the protein for Dendrobium candicum.Comprehensive analysis found that Ca(NO3)2 was the most conducive for Dendrobium candicum suspension culture.

参考文献/References:

[1]冉懋熊.名贵中药材绿色栽培技术[M].北京：科学技术文献出版社,2002.

[2]吴志刚,刘贤旺,张寿文.石斛属植物组织培养研究进展[J].中药研究与信息,2005(1)：23-25.

[3]胡忠,吉星和.黑节草种苗的大量培养[J].植物杂志,1979(3)：1.

[4]何静波,李勇.黑节草原胚体的繁殖[J].云南植物研究,1982,4(2)：211.

[5]张治国,刘骅,王黎.铁皮石斛原球茎增殖的培养条件研究[J].中草药,1992,23(8)：431.

[6]郭顺星,曹文岑,张集慧.铁皮石斛人工种子制作流程及发芽研究[J].中草药,1996,27(2)：105.

[7]徐红,李晓波,王铮涛,等.海南、广东省石斛属植物资源与种质保护研究[J].中国野生植物资源，1999(5)：24-27.

[8]秦廷豪.铁皮石斛的组织培养与快速繁殖[J].热带农业科学,2008,28(1)：25-29.

[9]李进进.铁皮石斛茎段离体初代培养研究[J].作物杂志,2010(1)：79-80.

[10]何涛,淳泽,汪天杰,等.铁皮石斛腋芽的快速繁殖[J].中国野生植物资源,2010,29(1)：58-61.

[11]蒋向辉,佘朝文,王善粉.不同激素浓度对铁皮石斛高效快繁体系的影响[J].江苏农业科学,2009(6)：76-78.

[12]汤亚飞,蔡时可,郑锦荣.铁皮石斛组织培养与快速繁殖(简报)[J].亚热带植物科学,2010,39(2)：74-75.

[13]Xu H,Lu J,Wang Z T,et al.The scavenging of reactive oxygen species by crude drugs and cultured tissues of five species of?Dendrohium?Sw[J].J Plant Resour Environ,2011,10(2)：35-37.

[14]Gao J,Jin R,Wu Y,et al.Comparative study of tissue cultured?Dendrobium protocorm on immunologrcal function [J].J Chin Med Mater,2002,25(7)：487-489.

[15]洪森荣,肖波,江静.KT和NAA对铁皮石斛带芽茎段生长发育的影响[J].江苏农业科学,2012,40(1)：55-56.

[16]陈希,黄丹丹.铁皮石斛的组织培养与快速繁殖研究进展[J].海峡药学,2011,23(8)：16-23.

[17]苏江,罗兴录,何铁光,等.铁皮石斛原球茎固体培养和液体悬浮培养动力学研究[J].西北农业学报，2007,16(4)：161-165.

[18]宋经元,郭顺星,肖培根.铁皮石斛原球茎液体悬浮培养的研究[J].中草药,2004,35(9)：1042-1046.

[19]Hahlbrock K,Scheel D,Logemann E,et al.Oligopeptide elicitor-mediated defense gene activation in cultured parsley cells[J].Proc Natl Acad Sci USA,1995,92(10)：4150-4157.

[20]魏小勇.铁皮石斛原球茎悬浮培养研究[J].现代中药研究与实践,2004,18(4)：7-11.

[21]韩晓红,段春红,阎贺静,等.铁皮石斛原球茎液体悬浮培养研究进展[J].安徽农业科学,2010,38(20)：10570-10572.

[22]林丛发,钟爱青,林云斌,等.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报,2007,19(1)：84-86.

[23]苏钛,张晓南.液体悬浮培养促进铁皮石斛原球茎高效诱导、增殖的研究[J].中国野生植物资源,2009,28(4)：54-56.

[24]韦晓新,苏江,何铁光.因素对铁皮石斛原球茎生长和多糖积累的影响[J].广西农业科学,2008（9）：601-606.

[25]何铁光,杨丽涛,李杨瑞,等.蔗糖对铁皮石斛原球茎生长与多糖积累的影响[J].安徽农业科学,2007,19(1)：84-86,91.

[26]贾薇,唐庆九.硫酸苯酚法测定云芝多糖含量的效果[J].河南农业科学,1999（8）：24-25.

[27]杨正坤,王秀丽等.考马斯亮蓝染色法测定大豆茎叶中蛋白质含量[J].湖北农业科学,2012（10）：4610-4613.

[28]宋经元,郭顺星,肖培根.氮源和真菌诱导子对铁皮石斛原球茎悬浮培养的影响[J].云南植物研究,2008,30(1)：105-109.

[29]高洪波,李敬蕊,章铁军,等.甘氨酸和谷氨酸与钼配施对生菜品质的影响[J].西北植物学报,2010,30(5)：968-973.

[30]荆留萍,杜双田,金凌云,等.8种物质对蛹虫草液体发酵中虫草素及多糖含量的影响[J].西北农林科技大学学报,2010,38(11)：156-160.

[31]Flowers T J.Salt tolerance in Suaeda maritima (L.) Dum：the effect of sodium chloride on growth,respiration,and soluble enzymes in a comparative study with Pisum sativumL[J].J Exp Bot,1972,23：310-321.

[32]李宗菊,桂明英.谷氨酸在无糖组织培养中的应用[J].西南农业学报,1999,12(3)：45-49.


相似文献/References:

[1]林群英,吴亮亮,张锋伦,等.氮源对金针菇菌糠栽培杏鲍菇的改良作用研究[J].北方园艺,2013,37(22):151.
　LIN Qun-ying,WU Liang-liang,ZHANG Feng-lun,et al.Study on the Modified Effect of Nitrogen Source on Culture of Pleurotus eryngii with Mushroom Bran of Flammulina velutipes [J].Northern Horticulture,2013,37(07):151.
[2]黄作喜,张 杨,颜小玉,等.铁皮石斛原球茎高效增殖体系的构建[J].北方园艺,2014,38(14):107.
　HUANG Zuo-xi,ZHANG Yang,YAN Xiao-yu,et al.Establishment for a Highly Efficient Proliferation System of Protocorm of Dendrobium officinale Kimura et Migo[J].Northern Horticulture,2014,38(07):107.
[3]胡俊杰,金伊洙.不同碳源和氮源对生菜褐斑病菌生长的影响[J].北方园艺,2014,38(01):114.
　HU Jun-jie,JIN Yi-zhu.Effect of Different Carbon and Nitrogen Sources on the Growth of Lettuce Carpospores[J].Northern Horticulture,2014,38(07):114.
[4]潘梅,王景飞,姜殿强,等.铁皮石斛丛生芽增殖培养条件的优化[J].北方园艺,2013,37(13):128.
　PAN Mei,WANG Jing-fei,JIANG Dian-qiang,et al.Clustered Buds Proliferation Optimization of Culture Conditions of Dendrobium candidum[J].Northern Horticulture,2013,37(07):128.
[5]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
　GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(07):148.
[6]梁淑颖,曾令杰.组培条件下铁皮石斛幼芽分蘖和生根的影响因素研究[J].北方园艺,2013,37(14):112.
　LIANG Shu-ying,ZENG Ling-jie.Study on the Factors Affecting Tillering and Rooting of Buldet of Dendrobium officinale Kimura et Migo in Culture Medium[J].Northern Horticulture,2013,37(07):112.
[7]唐政,陈小香,黄献珠.混合酶提法提取铁皮石斛中石斛多糖的优化工艺研究[J].北方园艺,2014,38(06):132.
　TANG Zheng,CHEN Xiao-xiang,HUANG Xian-zhu.Optimization of the Extraction Technology of Dendrobium Polysaccharides From Dendrobium officinale by Mixed Enzyme[J].Northern Horticulture,2014,38(07):132.
[8]周会明,张焱珍,席亚丽,等.五株荷叶离褶伞碳氮源及适合发酵菌株的筛选[J].北方园艺,2014,38(09):159.
　ZHOU Hui-ming,ZHANG Yan-zhen,XI Ya-li,et al.Screening of Different Carbon and Nitrogen Sources and Suitable for Fermentation on Five Strains of Lyophyllum decastes[J].Northern Horticulture,2014,38(07):159.
[9]金岩.不同碳源和氮源对杏斑点病病菌的影响[J].北方园艺,2013,37(07):140.
　JIN Yan.Effects of Different Carbon and Nitrogen Sources on Phyllosticta chlorospora McAlp of Prunus armeniaca L.[J].Northern Horticulture,2013,37(07):140.
[10]李国树,徐成东,尚正丽,等.五种果蔬提取物对铁皮石斛茎叶分化苗促根效果的影响[J].北方园艺,2013,37(06):161.
　LI Guo-shu,XU Cheng-dong,SHANG Zheng-li,et al.Effect of Five Kinds of Fruit and Vegetable Extracts on the Root-inducing Effect of Seedlings Differentiated from Dendrobium officinale Stem[J].Northern Horticulture,2013,37(07):161.

备注/Memo

第一作者简介：练华山（1979-），男，农业推广硕士，讲师，农艺师，现主要从事蔬菜与花木等的教学与科研推广工作。E-mail：lhs8748@aliyun.com.

更新日期/Last Update: 2014-08-13