TANG Zheng,CHEN Xiao-xiang,HUANG Xian-zhu.Optimization of the Extraction Technology of Dendrobium Polysaccharides From Dendrobium officinale by Mixed Enzyme[J].Northern Horticulture,2014,38(06):132-134.
混合酶提法提取铁皮石斛中石斛多糖的优化工艺研究
- Title:
- Optimization of the Extraction Technology of Dendrobium Polysaccharides From Dendrobium officinale by Mixed Enzyme
- 文章编号:
- 1001-0009(2014)06-0132-03
- Keywords:
- Dendrobium officinale; extraction technology; dendrobium polysaccharides; cellulase; protopectinase
- 分类号:
- R 931.71
- 文献标志码:
- A
- 摘要:
- 以铁皮石斛为试材，采用纤维素酶与果胶酶的等量混合提取法，通过单因素试验与正交实验，研究了pH值、酶浓度、酶解时间、酶解温度等因素对石斛多糖提取效率的影响。结果表明：铁皮石斛多糖提取最佳工艺为pH 6.5、1%浓度的纤维素酶和果胶酶1∶1等量混合、50℃下提取135 min,在此条件下石斛多糖提取率为41.33%;说明该工艺是可行的，且具有较高的提取率。
- Abstract:
- Taking Dendrobium officinale as material,by single factor experiments and orthogonal experiment with the mixed enzyme,the influences of the enzyme dosage，the composition of mixed enzyme，enzymolysis time,enzymolysis temperature，and the value of pH were adopted,the optimization extraction process of dendrobium polysaccharides from Dendrobium officinalewas studied.The results showed that the optimal extraction technology was as follow：the mixed enzyme(cellulose∶protopectinase=1∶1) concentration was 1%,the pH value was 6.5,the enzymolysis temperature was 50℃ and the extracting time was 135 min．The yield of the dendrobium polysaccharides was 41.33%.The process was reasonable with high rates of extraction of Dendrobium polysaccharides．
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备注/Memo
第一作者简介：唐政（1977-），男，博士，现主要从事作物生态学与植物天然物质提取等研究工作。E-mail：bioecology74tang@yeah.net.