PAN Mei,WANG Jing-fei,JIANG Dian-qiang,et al.Clustered Buds Proliferation Optimization of Culture Conditions of Dendrobium candidum[J].Northern Horticulture,2013,37(13):128-130.
铁皮石斛丛生芽增殖培养条件的优化
- Title:
- Clustered Buds Proliferation Optimization of Culture Conditions of Dendrobium candidum
- 文章编号:
- 1001-0009（2013）13-0128-03
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 为筛选出铁皮石斛从生芽继代增殖的最适培养基和培养条件，以MS为基本培养基，研究比较了活性炭、植物激素、有机添加物以及温度和光照等因素对铁皮石斛生长状况的影响。结果表明：丛生芽继代增殖的最佳配方为MS+6BA 2.0 mg/L+NAA 0.2 mg/L+香蕉100 g/L+活性炭0.5 g/L+蔗糖30 g/L，丛生芽长势好，60d增殖系数达到7.16；最适培养温度为25℃，最佳的光照强度为2 000 lx。
- Abstract:
- In order to select optimal medium and culture conditions from clustered buds subculture proliferation of Dendrobium candidum，taking the MS as the basic medium,the effect of activated carbon，plant hormones，organic additives，temperature，light and other factors on growth of Dendrobium candidum were studied.The results showed that the best formola for clustered buds subculture proliferation was MS+6BA 2.0 mg/L+NAA 0.2 mg/L+banana 100 g/L+activated carbon 0.5 g/L+sucrose 30 g/L，proliferation coefficient reached 7.16 in 60 days;optimal temperature was 25℃，with optimal light intensity for 2 000 lx.
参考文献/References:
[1]林丛发,钟爱清,林云斌，等.铁皮石斛类原球茎增殖和分化的研究[J].江西农业学报,2007,19(1):84-86.
相似文献/References:
[1]黄作喜,张 杨,颜小玉,等.铁皮石斛原球茎高效增殖体系的构建[J].北方园艺,2014,38(14):107.
HUANG Zuo-xi,ZHANG Yang,YAN Xiao-yu,et al.Establishment for a Highly Efficient Proliferation System of Protocorm of Dendrobium officinale Kimura et Migo[J].Northern Horticulture,2014,38(13):107.
[2]潘梅,符瑞侃,黄赛,等.药用裸花紫珠微繁技术增殖培养基筛选研究[J].北方园艺,2014,38(04):96.
PAN Mei,FU Rui-kan,HUANG Sai,et al.Screening Study on Proliferation Medium of Micropropagation Technique for Callicarpa nudiflora[J].Northern Horticulture,2014,38(13):96.
[3]勾畅,武慧,将达,等.激素配比对不同基因型菊花再生体系的调控[J].北方园艺,2013,37(13):135.
GOU Chang,WU Hui,JIANG Da,et al.Effects of Different Hormone Combinations on the Regeneration of ?Four Different Chrysanthemum Genotypes[J].Northern Horticulture,2013,37(13):135.
[4]龚庆芳,赵 健,何金祥,等.遮荫对一年生铁皮石斛生长特性、光合特性及多糖含量的影响[J].北方园艺,2014,38(01):148.
GONG Qing-fang,ZHAO Jian,HE Jin-xiang,et al.Effects of Shade Treatment on the Growth and Photosynthetic Characteristics and Polysaccharides Content of Annual Dendrobium officinale [J].Northern Horticulture,2014,38(13):148.
[5]麻冬梅,王静,金凤霞,等.苜蓿子叶节离体再生体系的建立[J].北方园艺,2013,37(14):104.
MA Dong-mei,WANG Jing,JIN Feng-xia,et al.Establishment of Regeneration System in vitro from Cotyledonary Nodes of Medicago sativa L.[J].Northern Horticulture,2013,37(13):104.
[6]梁淑颖,曾令杰.组培条件下铁皮石斛幼芽分蘖和生根的影响因素研究[J].北方园艺,2013,37(14):112.
LIANG Shu-ying,ZENG Ling-jie.Study on the Factors Affecting Tillering and Rooting of Buldet of Dendrobium officinale Kimura et Migo in Culture Medium[J].Northern Horticulture,2013,37(13):112.
[7]唐政,陈小香,黄献珠.混合酶提法提取铁皮石斛中石斛多糖的优化工艺研究[J].北方园艺,2014,38(06):132.
TANG Zheng,CHEN Xiao-xiang,HUANG Xian-zhu.Optimization of the Extraction Technology of Dendrobium Polysaccharides From Dendrobium officinale by Mixed Enzyme[J].Northern Horticulture,2014,38(13):132.
[8]练华山,杨贵先,李焕秀.不同氮源对铁皮石斛原球茎液体悬浮培养的影响[J].北方园艺,2014,38(07):152.
LIAN Hua-shan,YANG Gui-xian,LI Huan-xiu.Effect of Different Nitrogen Sources on Dendrobium candicum Wall.ex Lindl Suspension Cultures[J].Northern Horticulture,2014,38(13):152.
[9]赵玉芬,储博彦,尹新彦,等.应用正交实验优化大花萱草试管苗生根的研究[J].北方园艺,2013,37(06):100.
ZHAO Yu-fen,CHU Bo-yan,YIN Xin-yan,et al.Study on the Optimization for the Tube Seedlings Rooting of Hemerocallis hybridas by Using the Orthogonal Experiment[J].Northern Horticulture,2013,37(13):100.
[10]杨 爽,王 勇.西藏产茅膏菜丛生芽诱导和增殖的研究[J].北方园艺,2013,37(06):102.
YANG Shuang,WANG Yong.Study on the Induction and Proliferation of Clustered Buds of Drosera peltata [J].Northern Horticulture,2013,37(13):102.
[11]杨平飞,吴明开,宋智琴,等.不同激素配比对铁皮石斛组培丛生芽增殖的影响[J].北方园艺,2016,40(06):106.[doi:10.11937/bfyy.201606026]
YANG Pingfei,WU Mingkai,SONG Zhiqin,et al.Effect of Medium of Different Hormone on Dendrobium officinale Buds Proliferation[J].Northern Horticulture,2016,40(13):106.[doi:10.11937/bfyy.201606026]
备注/Memo
第一作者简介：潘梅（1962-），女，广西隆安人，本科，高级园艺师，现主要从事植物组织培养及开发利用等工作。E-mail：panmei200@sina.com.