[1]李 光,余 霜,邓 银,等.金荞麦叶总黄酮含量测定方法研究[J].北方园艺,2013,37(17):154-156.
　LI Guang,YU Shuang,DENG Yin,et al.Study on the Determination Method of Flavonoids in Fagopyrum cymosum Complex Leaves[J].Northern Horticulture,2013,37(17):154-156.
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金荞麦叶总黄酮含量测定方法研究

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第37卷 期数: 2013年17期 页码: 154-156 栏目: 出版日期: 2013-09-15
Title:
Study on the Determination Method of Flavonoids in Fagopyrum cymosum Complex Leaves
文章编号:
1001-0009(2013)17-0154-03
作者:
李 光1; 2; 3; 余 霜1; 邓 银1; 周永红2; 陈庆富1
1.贵州师范大学 生命科学学院 植物遗传育种研究所，荞麦产业技术研究中心，贵州 贵阳 550001；
2.四川农业大学 小麦研究所，四川 温江 611130;
3.安顺学院 化学与生物农学系，贵州 安顺 561000
Author(s):
LI Guang1; 2; 3; YU Shuang1; DENG Yin1; ZHOU Yong-hong2; CHEN Qing-fu1
1.Research Center of Buckwheat Industry Technology,Institute of Plant Genetic and Breeding,School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550001;
2.Triticeae Research Institute,Sichuan Agricultural University,Wenjiang,Sichuan 611130;
3.College of Chemistry and Biology,Anshun University,Anshun,Guizhou 561000
关键词:
金荞麦叶; 黄酮; 测定方法
Keywords:
Fagopyrum cymosum complex leaves; flavonoids; determination method
分类号:
S 567
文献标志码:
B
摘要:
以金荞麦叶为试材，采用超声波辅助法提取金荞麦叶中的黄酮类化合物，研究了基于亚硝酸钠-硝酸铝法的金荞麦叶总黄酮含量测定的改良方法。结果表明：该方法稳定性好，操作简单方便，可适用于金荞麦叶提取液中总黄酮含量的测定。
Abstract:
Taking Fagopyrum cymosum complex leaves as material,ultrasonic extraction method was used to extract flavonoids from Fagopyrum cymosum complex leaves,and a new determination method base on NaNO2-Al (NO3)3-NaOH spectrophotometric method was built.The results showed that this method was convenient,accurate,and reproducible,which could be used for flavonoids content determination of other parts in Fagopyrum cymosum complex.

参考文献/References:

[1] 延玺,刘会青,邹永青,等.黄酮类化合物生理活性及合成研究进展[J].有机化学,2008,28(9):1534-1544.
[2] 李安仁.中国植物志[M].北京:科学出版社,1998.
[3] 李敏晶,游景艳,刘忠英,等.微波辅助流动萃取槐花中的黄酮类成分[J].高等学校化学学报,2004(5):850-852.
[4] 林静,刘群,李艳冬,等.金荞麦愈伤组织诱导及其总黄酮含量测定方法的建立[J].西南民族大学学报(自然科学版),2010(2):230-234.

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备注/Memo

第一作者简介：李光（1980-），男，河南商丘人，博士，副教授，研究方向为作物遗传育种及天然产物提取。E-mail:lg20029@126.com.
责任作者：陈庆富（1966-），男，教授，博士生导师，研究方向为作物遗传育种及天然产物提取。E-mail:cqf1966@163.com.
基金项目：国家自然科学基金资助项目(31060207，31171609);
               国家现代农业产业技术体系专项资金资助项目(CARS-08-A4)；
               贵州省农业科技攻关资助项目(黔科合NY字［2010］3094);
               贵州省科技创新团队资助项目(黔科合人才团队(2011)4007)；
               贵阳市科技计划资助项目（筑科合同［2011102］1-12）。
收稿日期：2013-04-15

更新日期/Last Update: 2014-08-12