HAN Yong,WANG Xian-zhi,ZHANG Hong.Study on Deproteinization of Exopolysaccharide from Poria cocos Mycelia[J].Northern Horticulture,2013,37(07):151-153.
茯苓菌丝体胞外多糖的脱蛋白方法研究
- Title:
- Study on Deproteinization of Exopolysaccharide from Poria cocos Mycelia
- 文章编号:
- 1001-0009(2013)07-0151-03
- 分类号:
- S 567.3+2
- 文献标志码:
- A
- 摘要:
- 以蛋白质脱除率和多糖损失率为考察指标，研究比较了Sevag法、三氯乙酸法、酶法、酶-Sevag法对茯苓菌丝体胞外多糖脱蛋白效果的影响。结果表明：酶-Sevag法是较佳的脱除茯苓菌丝体胞外多糖中蛋白质的方法，酶用量1.5%，温度50℃，pH 6.5，脱蛋白2 h，Sevag法脱蛋白3次，蛋白质脱除率为87.43%，多糖损失率为10.97%。
- Abstract:
- Taking the rate of removed protein and rate of lost polysaccharide as indexes,the effect of sevag method，trichloroacetic acid method，enzyme method，enzyme-Sevag method on deproteinization of exopolysaccharide from Poria cocos mycelia were studied.The results showed that the enzyme-Sevag method was the best method of removing protein from the exopolysaccharide of Poria cocos mycelia.The optimal conditions of deproteinization were as follows：1.5% enzyme，temperature at 50℃，pH value 6.5，deproteinization time 2 h，Sevag deproteinization 3 times treatment.The rate of removed protein and the rate of lost polysaccharide were 87.43% and 10.97%，respectively.
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备注/Memo
第一作者简介：韩勇（1978-），男，硕士，助教，现主要从事微生物发酵工程的教学与研究工作。E-mail：swxyhy@163.com.