LI Yu-zhong,WANG Tong,TENG Tao,et al.Separation of Contaminating Microorganism from Culture Medium of Pleurorus ostreatus and Its Effect on Hypha Growth of P.ostreatus[J].Northern Horticulture,2013,37(23):155-158.
平菇栽培基质中污染菌的分离及其对平菇菌丝生长的影响
- Title:
- Separation of Contaminating Microorganism from Culture Medium of Pleurorus ostreatus and Its Effect on Hypha Growth of P.ostreatus
- 文章编号:
- 1001-0009（2013）23-0155-04
- 分类号:
- S 646.1+4
- 文献标志码:
- A
- 摘要:
- 从衡阳市蘑菇生产厂房采集正在出菇（但出菇较差）的平菇培养基质，带回实验室经分离、纯化和保存，根据菌落、菌丝、孢子、细胞的形态进行初步鉴定，并在PDA平板上用分离纯化菌株与平菇菌丝进行了对峙接种试验，观察污染菌对平菇菌丝生长的影响。结果表明：平菇培养共分离获得8个菌株，编号分别为PoD1～PoD8，经形态初步鉴定，PoD1、PoD2和PoD3分别为黄色单胞菌属（Flavimonas）、枯草芽孢杆菌（Bacillus subtilis）和假单孢菌属（Pseudomonas）,PoD4和PoD5为红酵母属（Rhodolorula）的2个种，PoD6为裂殖酵母属（Schizosaccharomyces）,酵母菌PoD7和PoD8分别为好食脉孢菌（Neurospora sitophila）和灰绿曲霉（Aspergullus glaucus）。对峙接种结果显示，丝状真菌对平菇菌丝生长的抑制效果最明显,其次为细菌,分离的酵母菌对平菇菌丝生长的影响最小。
- Abstract:
- Culture medium of Pteurorus ostreatus were collected from mushroom production plant in Hengyang area and took back to the laboratory to deal.The strains，separated from the medium，were purified and identified by colony and microscopic morphology.Then mycelium of each one separated strains and P.ostreatus were inoculated on the same PDA flat to study the effects of isolated strains on the growth of P.ostreatus.The results showed that eight strains，numbered PoD1~PoD8 were isolated and identified by morphology，PoD1，PoD2 and PoD3 were Flavimonas sp.，Bacillus subtilis and Pseudomonas sp.respectively；PoD4 and PoD5 were Rhodolorula spp.，PoD6 was Schizosaccharomyces sp.；PoD7 and PoD8 were Neurospora sitophila and Aspergillus glaucus respectively.The results of confrontation inoculation showed that moulds had significant inhibition effect on the growth of P.ostreatus，then the bacteria had a certain inhibition effect，the last were yeasts.
参考文献/References:
[1] Del Toro G V，Vega R C，Garin-aguilar M E,et al.Biological quality of proteins from three strains of Pleurotus spp.[J].Food Chemistry,2006,94(4):494-497.
相似文献/References:
[1]刘志曦,李志强,魏海莲,等.平菇ISSR遗传多样性分析[J].北方园艺,2014,38(12):94.
LIU Zhi-xi,LI Zhi-qiang,WEI Hai-lian,et al. Phylogenetic Analysis of Oyster Mushrooms by ISSR Marker[J].Northern Horticulture,2014,38(23):94.
[2]刘红,朱咏莉,李萍萍.不同配比醋糟基质对矮牵牛生长的影响[J].北方园艺,2013,37(14):65.
LIU Hong,ZHU Yong-li,LI Ping-ping.Effect of Different Ratios of Vinegar Residue Substrates on Growth of Petunia hyhrida Vilm[J].Northern Horticulture,2013,37(23):65.
[3]史正军,范雅文.轻型屋顶绿化植物栽培基质配方筛选研究[J].北方园艺,2013,37(14):73.
SHI Zheng-jun,FAN Ya-wen.Study on the Substrate Formulation Screening of Lightweight Roof Greening[J].Northern Horticulture,2013,37(23):73.
[4]陈艳露,周 莉,黄麟淇,等.抗木霉平菇菌株的筛选及其生产性能评价[J].北方园艺,2013,37(17):145.
CHEN Yan-lu,ZHOU Li,HUANG Lin-qi,et al.Screening and Evaluation of Oyster Mushroom Strains Resistant to Trichoderma Disease[J].Northern Horticulture,2013,37(23):145.
[5]陈 靓,姚方杰.平菇新品种“吉平1号”的选育[J].北方园艺,2014,38(08):147.
CHEN Liang,YAO Fang-jie.Breeding of A New Pleurotus Cultivar ‘Jiping No.1’[J].Northern Horticulture,2014,38(23):147.
[6]梁悦萍,唐道城.栽培基质对郁金香鳞茎形态发育及还原糖含量的影响[J].北方园艺,2013,37(11):59.
LIANG Yue-ping,TANG Dao-cheng.Effects of Different Culture Substrates on Morphological Development and Reducing Sugar Content in Tulip Bulbs[J].Northern Horticulture,2013,37(23):59.
[7]付月月,杨洪一.平菇病毒dsRNA的提取及脱除[J].北方园艺,2013,37(12):111.
FU Yue-yue,YANG Hong-yi.Extraction and Elimination of Virus of dsRNA from Pleurotus ostreatus[J].Northern Horticulture,2013,37(23):111.
[8]刘遂飞,张志红,孙桂琴,等.不同中药渣组合替代棉籽壳栽培平菇试验[J].北方园艺,2013,37(12):155.
LIU Sui-fei,ZHANG Zhi-hong,SUN Gui-qin,et al.Research on the Chinese Medicine Residue Instead of the Cotton Seed Hull in Planting Pleurotus ostreatus[J].Northern Horticulture,2013,37(23):155.
[9]陈仁玉,刘朝贵,方琼.有机氮对平菇原基分化期胞外酶的影响[J].北方园艺,2012,36(01):153.
CHEN Ren-yu,LIU Chao-gui,FANG Qiong.Effects of Organic Nitrogen on the Activity of Extracellular Enzymes During Primordium Formation of Pleurotus ostreatus[J].Northern Horticulture,2012,36(23):153.
[10]许杰,林电,林建明.基质在热带兰栽培过程中的养分释放研究[J].北方园艺,2012,36(02):154.
XU Jie,LIN Dian,LIN Jian-ming.The Matrix’s Nutrient Release in Tropical Orchid Cultivation[J].Northern Horticulture,2012,36(23):154.
[11]尹淑丽,梁然,李书生,等.梨木屑基质对平菇子实体氨基酸组成及营养价值的影响[J].北方园艺,2022,(11):112.[doi:10.11937/bfyy.20214576]
YIN Shuli,LIANG Ran,LI Shusheng,et al.Effects of Pear Sawdust Cultivation Substrate on Amino Acid Composition and Nutritional Value in Pleurotus ostreatus Fruit Bodies[J].Northern Horticulture,2022,(23):112.[doi:10.11937/bfyy.20214576]
[12]张春艳,魏雅冬,胡畔.杨木屑与玉米秸秆配方基质对平菇生长、营养价值及产量的影响[J].北方园艺,2023,(20):117.[doi:10.11937/bfyy.20231402]
ZHANG Chunyan,WEI Yadong,HU Pan.Effects of Compound Substrates of Poplar Sawdust and Corn Straw on Growth，Nutritional Value and Yield of Pleurotus ostreatus[J].Northern Horticulture,2023,(23):117.[doi:10.11937/bfyy.20231402]
备注/Memo
第一作者简介：李玉中（1978-），男，山东聊城人，博士，副教授，现主要从事微生物及植物病害生物防治等教学与科研工作。E-mail：yzl_2002@163.com.