ZHANG Zhi,LIANG Yi,DING Hai-feng,et al.Study on Cloning and Expression of atp9 Gene in Carrot and Its Relationship to Cytoplasmic Male Sterility[J].Northern Horticulture,2012,36(24):140-144.
胡萝卜atp9基因的克隆表达及细胞质雄性不育的相关性研究
- Title:
- Study on Cloning and Expression of atp9 Gene in Carrot and Its Relationship to Cytoplasmic Male Sterility
- 文章编号:
- 1001-0009（2012）24-0140-05
- 关键词:
- 胡萝卜; atp9基因细胞质雄性不育; RT-PCR; 实时荧光定量PCR
- Keywords:
- carrot; atp9 gene; cytoplasmic male sterility; RT-PCR; real-time PCR
- 分类号:
- S 631.2
- 文献标志码:
- A
- 摘要:
- 以胡萝卜及其它植物atp9基因序列信息设计引物，分离并克隆了胡萝卜细胞质雄性不育相关的线粒体基因片段，并利用半定量RT-PCR和Real-time PCR技术研究了该基因在不育系和相应保持系中的表达情况。结果表明：与保持系相比，atp9基因在不育系中发生了若干碱基的置换及缺失；基因表达分析表明，不育系atp9基因在花蕾的前3个发育时期表达量明显较低，并在大花蕾期超过了保持系，但是在盛花期其表达量又低于保持系。推测atp9基因的结构变异和异常表达与胡萝卜细胞质雄性不育有着密切关系。
- Abstract:
- Specific primers were designed according to the atp9 gene in carrot and other plants，carrot mitochondrion gene fragments associated with cytoplasmic male sterility were cloned either.The results showed that carrot atp9 gene contains a many base pair’s substitution and deletion in sterile line compared with maintainer line.The expression analysis using semi-quantitative RT-PCR and Real-time PCR demonstrates that expression of atp9 gene in carrot sterile line was significantly lower than in maintainer line besides the big bud stage.These researches indicated that atp9 gene mutation and its abnormal expression had a closely relationship with carrot cytoplasmic male sterility.
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备注/Memo
第一作者简介：张智（1980-），男，硕士，助理研究员，现主要从事胡萝卜和洋葱遗传研究等工作。E-mail:zhangzhi_zz@126.com.