HOU Xiao-jie.Detection of Indigenous Endophytic Bacteria in Jujube Seedlings Germinated From Seeds[J].Northern Horticulture,2012,36(22):108-111.
枣树实生苗固有内生细菌的检测
- Title:
- Detection of Indigenous Endophytic Bacteria in Jujube Seedlings Germinated From Seeds
- 文章编号:
- 1001-0009（2012）22-0108-04
- Keywords:
- Ziziphus jujuba; endophytic bacteria; detection; optical microscope; scanning electronic microscope; 16S rDNA
- 分类号:
- S 665.1
- 文献标志码:
- A
- 摘要:
- 通过光学显微镜、扫描电镜观察和分子检测方法对阜平大枣实生组培苗体内的固有内生细菌进行了研究。结果表明：在光学显微镜1 000倍下可观察到球状、短杆状和杆状细菌，其大小分别为1.5~1.8 μm、(2.2~2.9) μm×(1.1~1.9) μm、(3.7~4.4) μm×(1.6~1.9) μm；扫描电镜下拍摄到杆状细菌，大小为(3.5~4.0) μm×(1.5~2.0) μm；应用细菌通用引物pf27/1525扩增该实生苗体内的16S rDNA时，检测到大小为1.5 kb的细菌DNA特异性条带。
- Abstract:
- The indigenous endophytic bacteria in the tissue culture seedlings germinated from seeds of Ziziphus jujuba var.fupingdazao were studied using observation with optical microscope and scanning electronic microscope (SEM)，and detection of specific 16S rDNA fragment.The results showed that a large quantity of endophytic bacteria in jujube seedlings were detected under optical microscope with a magnification of 1 000 times，and round，short rod or rod bacterial cells with the size of 1.5~1.8 μm，(2.2~2.9) μm×(1.1~1.9) μm and (3.7~4.4) μm×(1.6~1.9) μm，respectively，were observed.Rod bacteria were found by SEM as well.The 16S rDNA fragment of 1.5 kb was amplified from the total DNA of seedlings of jujube with the bacterial primer pair pf27/1525.
参考文献/References:
[1]Bacon C W，Hinton D M.Isolation and culture of endophytic bacteria and fungi.In：Hurst C J，Knudsen G R，McInerney M J，et al. Manual of Environmental Microbiology[M].Washington (DC):ASM Press,1996:413-421. [2]韩继刚,宋末.植物内生细菌研究进展及其应用潜力[J].自然科学进展,2004(4):374-379. [3]Fisher P J，Petrini O，Lappinscott H M.The distribution of some fungal and bacterial endophytes in maize(Zea maysL)[J].New Phytologist,1992,122:299-305. [4]Bacilio J M，Aguilar F S，Del Valle M V，et al.Endophytic bacteria in rice seeds inhibit early colonization of roots by Azospirillum brasilense[J].Soil Biology and Biochemistry,2001,33:167-172. [5]邱思鑫,何红,阮宏椿,等.具有抑菌促生作用的植物内生细菌的筛选[J].应用与环境生物学报,2004,10(5):655-659. [6]Mukhopadhyay N K，Garrison N K，Hinton D M，et al. Identification and characterization of bacterial endophytes of rice[J].Mycopathologia,1996,134:151-159. [7]田雪亮,单长卷.黄瓜种子及幼苗内生细菌分离鉴定[J].安徽农业科学,2006,34(8):1574-1575. [8]张志元,罗永兰,官春云.油菜种子内生菌的检测及杀菌消毒处理方法[J].湖北农业科学，2005(1):52-55. [9]Mundt J O，Hinkle N F.Bacteria within ovules and seeds[J].Applied and Environmental Microbiology,1976,32(5):694-698. [10]Garbaye J.Helper bacteria：a new dimension to the micorrhizal symbiosis[J].New phytologist,1994,128:197-210. [11]Shishido M，Breuil C，Chanway C P.Endophytic colonization of spruce by plant growth-promoting rhizobacteria[J].FEMS Microbiology Ecology,1999,29:191-196. [12]Chanway C P，Shishido M，Nairn J，et al.Endophytic colonization and field responses of hybrid spruce seedlings after inoculation with plant growth-promoting rhizobacteria[J].Forest Ecology and Management,2000,133:81-88. [13]Fernando E V，Francisco P，Catherine A M，et al. Fungal endophytes in green coffee seeds[J].Mycosystema，2008，27(1):75-84. [14]Golubev V I，Kulakovskaia T V，Golubeva E V.Pseudozyma fusiformata BKM Y-2821-a producer of antifungal glycolipid[J].Mikrobiologiia,2001,70(5):642-646. [15]Avis T J，Belanger R R.Mechanisms and means of detection of biocontrol activity of Pseudozymayeasts against plantpathogenic fungi[J].FEMS Yeast Research,2002(2):5-8. [16]Bal A S，Chanway C P.Isolation and identification of endophytic bacteria from lodgepole pine and western red cedar[EB/OL].Auburn University website，available：http://www.ag.auburn.edu/argentina/pdfmanuscripts/bal.2000. [17]Cankar K，Kraigher H，Ravnikar M, et al. Bacterial endophytes from seeds of Norway spruce (Picea abiesL.Karst)[J].FEMS Microbiology Letters,2005,244:341-345. [18]李永,田国忠,朴春根,等.我国几种植物植原体的快速分子鉴别与鉴定的研究[J].植物病理学报,2005,35(4):293-299. [19]张德民,黄志勇,杨惠芳.紫色非硫细菌Rhodocista属一新分离株的鉴定及其系统学研究[J].微生物学报,2000,40(1):14-20. [20]Dbereiner J.Recent changes in concepts of plant bacteria interactions：endophytic N2 fixing bacteria[J].Ciencia e Cultura (Sao Paulo),1992,44:310-313. [21]Dong Z，Canny M J，McCully M E，et al. A nitrogen-fixing endophyte of sugarcane stems (A new role for the apoplast)[J].Plant Physiology,1994,105:1139-1147. [22]James E K，Reis V M，Olivares F L，et al.Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus[J].Journal of Experimental Botany,1994,45:757-766. [23]Dbereiner J，Baldani V L D，Reis V M.Endophytic occurence of diazotrophic bacteria in non-leguminous crops.In I：Fendrik M，Del Gallo J，Vanderleyden M，et al. Azospirillum VI and Related Microorganisms[J].Springer-Verlag,Berlin,Germany,1995:3-14.Mikrobiologia,70:642-646(in Russian). [24]Taechowisan T，Peberdy J F，Lumyong S.Isolation of endophytic actinomycetes from selected plants and their antifungal activity[J].World Journal of Microbiology and Biotechnology,2003,19:381-385. [25]de Souza A O，Pamphile J A，de Mello Sartori Cardoso da Rocha C L，et al.Plant-microbe interactions between maize (Zea maysL.) and endophytic microrganisms observed by Scanning Electron Microscopy[J].Acta Scientiarum Biological Sciences,2004,26(3):357-359. [26]Amann R I，Ludwig W，Schleifer K H.Phylogenetic identification and in situ detection of individual microbial cells without cultivation[J].Microbiology and Molecular Biology Reviews,1995,59(1):143-169. [27]Tyson G W，Chapman J，Hugenholtz P，et al.Community structure and metabolism through reconstruction of microbial genomes from the environment[J].Nature,2004,428:37-43. [28]黎起秦,罗宽.番茄青枯病内生拮抗细菌的筛选[J].植物病理学报,2003,33(4):364-367. [29]何红,邱思鑫,胡方平.植物内生细菌生物学作用研究进展[J].微生物学杂志,2004,5(3):40-45.
相似文献/References:
[1]池振江,李 进,孙 洁,等.五种生物农药对新疆枣树叶螨室内毒力和田间药效试验[J].北方园艺,2014,38(12):106.
CHI Zhen-jiang,LI Jin,SUN Jie,et al.Indoor Toxicology and Field Efficacy of Five Biological Pesticides for Controlling Jujube Tersnyhus truncatus in Xinjiang[J].Northern Horticulture,2014,38(22):106.
[2]张琦,白团辉,吴翠云,等.红枣直播建园不同树形产量与品质调查研究[J].北方园艺,2013,37(14):18.
ZHANG Qi,BAI Tuan-hui,WU Cui-yun,et al.Investigation on Yield and Quality of Jujube in Different Tree Shapes in Direct Seeding and Orchard Construction[J].Northern Horticulture,2013,37(22):18.
[3]张存智,魏天军,魏鹏,等.灵武长枣高效继代增殖体系的建立[J].北方园艺,2012,36(16):95.
ZHANG Cun-zhi,WEI Tian-jun,WEI Peng,et al.Establish the Efficient System of Subculture Proliferation About Zizyphus jujube Mill cv.Lingwuchangzao[J].Northern Horticulture,2012,36(22):95.
[4]王晓玲,胡亚岚,毛丽衡.不同枣品种抗寒性的比较[J].北方园艺,2012,36(19):1.
WANG Xiao-ling,HU Ya-lan,MAO Li-heng.The Comparison of Cold Resistance Among Varieties of Chinese Jujube[J].Northern Horticulture,2012,36(22):1.
[5]李荣华,阎旭东,赵松山.称重法快速鉴定枣耐盐性的研究[J].北方园艺,2012,36(11):30.
LIRong-hua,YANXu-dong,ZHAOSong-shan.StudyonWeighingMethodtoRapidIdentificationtheSalt-toleranceofJujube[J].Northern Horticulture,2012,36(22):30.
[6]陈莹莹,苑赞,赵锦,等.枣转录因子ERF 3′端序列克隆及生物信息学分析[J].北方园艺,2014,38(22):93.
CHEN Ying-ying,YUAN Zan,ZHAO Jin,et al.Cloning and Bioinformatic Analysis of the 3′-end of ERF Gene in Ziziphus jujuba[J].Northern Horticulture,2014,38(22):93.
[7]白 杰,高红月,王大震,等.枣的栽培管理和病虫害防治[J].北方园艺,2015,39(05):51.[doi:10.11937/bfyy.201505017]
[J].Northern Horticulture,2015,39(22):51.[doi:10.11937/bfyy.201505017]
[8]卜娇迪,陈莹莹,刘孟军,等.枣甘油醛-3-磷酸脱氢酶基因的克隆及表达分析枣甘油醛-3-磷酸脱氢酶基因的克隆及表达分析[J].北方园艺,2015,39(13):102.[doi:10.11937/bfyy.201513030]
BU Jiaodi,CHEN Yingying,LIU Mengjun,et al.Cloning and Expression Analysis of Glyceraldehyde-3-phosphate Dehydrogenase From Ziziphus jujuba[J].Northern Horticulture,2015,39(22):102.[doi:10.11937/bfyy.201513030]
[9]魏 天 军,吴 云 峰,李 百 云.宁夏枣疯病植原体分子检测[J].北方园艺,2010,34(11):0.[doi:10.11937/bfyy.2010011053]
WEI Tian-jun,WU Yun-feng,LI Bai -yun.Molecular Detection of Phytoplama of Jujube Witches Broom in Ningxia Autonomous Region[J].Northern Horticulture,2010,34(22):0.[doi:10.11937/bfyy.2010011053]
[10]李梦钗,武亚敬,汤富彬.枣和板栗果实中农药残留分析[J].北方园艺,2016,40(14):133.[doi:10.11937/bfyy.201614033]
LI Mengchai,WU Yajing,TANG Fubin.Analysis of Chemical Residues in Jujube and Chestnut Fruit[J].Northern Horticulture,2016,40(22):133.[doi:10.11937/bfyy.201614033]
备注/Memo
作者简介：侯晓杰（1981-），女，河北衡水人，博士，讲师，研究方向为植物病害生物防治。E-mail：houxiaojie23@163.com.