[1]陈莹莹,苑赞,赵锦,等.枣转录因子ERF 3′端序列克隆及生物信息学分析[J].北方园艺,2014,38(22):93-97.
　CHEN Ying-ying,YUAN Zan,ZHAO Jin,et al.Cloning and Bioinformatic Analysis of the 3′-end of ERF Gene in Ziziphus jujuba[J].Northern Horticulture,2014,38(22):93-97.
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枣转录因子ERF 3′端序列克隆及生物信息学分析

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第38卷 期数: 2014年22期 页码: 93-97 栏目: 出版日期: 2014-11-30
Title:
Cloning and Bioinformatic Analysis of the 3′-end of ERF Gene in Ziziphus jujuba
文章编号:
1001-0009（2014）22-0093-05
作者:
陈莹莹1; 苑赞1; 赵锦2; 刘孟军1
1.河北农业大学 中国枣研究中心，河北 保定 071001；
2.河北农业大学 生命科学学院，河北 保定 071001
Author(s):
CHEN Ying-ying1; YUAN Zan1; ZHAO Jin2; LIU Meng-jun1
1.Research Center of Chinese Jujube,Agricultural University of Hebei,Baoding,Hebei 071001;
2.College of Life Science,Agricultural University of Hebei,Baoding,Hebei 071001
关键词:
枣; ZjERF; 3′端序列克隆; 生物信息学分析
Keywords:
Ziziphus jujuba; ZjERF; the 3′-end of ZjERF cloning; bioinformatic analysis
分类号:
Q 785；S 665
文献标志码:
A
摘要:
以枣为试材，根据 GenBank 中登录的苹果、拟南芥等植物EFR保守序列设计引物，采用同源克隆法克隆出该基因3′端序列并对其编码的蛋白进行了生物信息学分析。结果表明：该试验克隆获得1条长度为959 bp的条带；经过序列分析，该序列包含典型的 AP2/EREBP保守结构域，与桑树、欧洲山毛榉和欧洲栗ERF基因序列相似度分别为79%、78%、77%；系统进化树分析显示，枣的ZjERF与可可树、陆地棉和海岛棉的AP2/EREBP亲缘关系较近，属于ERF家族的 B2 亚群；此外，还用Swiss Model程序（http://au.expasy.org/tools/）预测了ZjERF基因编码蛋白的三维结构。
Abstract:
Taking Ziziphus jujuba as material,the fragment of ERF gene contained 3′-end of ZjERF was first isolated by homologous gene cloning method on the basis of the homologous genes of Malus domestica,Arabidopsis thaliana and others in GenBank.The results showed that a 959 bp fragment was amplified.Conservation analysis of ZjERF showed that it had a typical zone of AP2/EREBP；similarity analysis showed that the protein had highly homologous to the ZjERF of Morus alba, Fagus sylvatica and Castanea sativa with homologous of 79%,78% and 77% respectively；Phylogenic analysis showed that ERF of species in jujube had a closer relationship with Theobroma cacao,Gossypium hirsutum,Gossypium barbadense and ZjERF blonged to B2 group of ERF family.Tertiary structure of ZjERF was speculated by Swiss Model program（http://au.expasy.org/tools/）.

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备注/Memo

第一作者简介：陈莹莹(1987-)，女，河北石家庄人，硕士研究生，研究方向为果树活性物质与功能性食品。E-mail：wanying-1202@163.com.

责任作者：刘孟军(1965-)，男，河北保定人，博士，教授，研究方向为干果种质资源与分子辅助育种。E-mail：lmj1234567@aliyun.com.
基金项目：国家科技支撑计划资助项目(2013BAD14B030106)；河北省科技支撑计划资助项目（11230606D-6）。
收稿日期：2014-07-16

更新日期/Last Update: 2014-12-12