WANG Dian-pei,ZHANG Sheng-hong,LI Jian-hua.Optimization of Extraction Conditions of Xanthophyll from Tagetes erecta L. by Response Surface Methodology[J].Northern Horticulture,2012,36(22):111-114.
响应曲面法优化万寿菊叶黄素的提取工艺
- Title:
- Optimization of Extraction Conditions of Xanthophyll from Tagetes erecta L. by Response Surface Methodology
- 文章编号:
- 1001-0009(2012)22-0111-04
- 分类号:
- TS 264.4
- 文献标志码:
- A
- 摘要:
- 以乙酸乙酯为提取溶剂，以提取时间、提取温度以及液料比为自变量，采用Box-Benhnken中心组合设计原理和响应曲面分析法，研究了各因素及其交互作用对万寿菊叶黄素提取的影响。结果表明：叶黄素提取工艺最佳条件为：提取时间54.32 min，提取温度32.09℃，液料比 (mL∶g)为16.15∶1，吸光度的预测值为2.357，当选取提取时间55 min，提取温度32℃，液料比 (mL∶g)为16.15∶1的实际条件时，吸光度实际值为2.356。该试验所得工艺参数准确，可用于万寿菊叶黄素的提取生产。
- Abstract:
- With Ethyl acetate as extraction solvent，Box-Benhnken central composite design and response surface methodology were used to explore the effects of extraction time，extraction temperature and liquid-material ratio as well as their cross-interactions on extraction rate of xanthophyll from Tagetes erecta L.The results showed that the optimal extraction processing parameters were extraction time of 54.32 minutes，extraction temperature of 32.09℃ and liquid-material ratio of 16.15∶1，in which the forecast value was 2.357.Under the actual extraction conditions such as extraction time of 55 minutes，extraction temperature of 32℃ and liquid-material ratio of 16.15∶1，the absorbance value of xanthophyll was 2.356.The processing parameters achieved from these experiments were accurate and could be applied to the extraction and production of xanthophyll from T.erecta L.
参考文献/References:
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备注/Memo
第一作者简介：汪殿蓓(1968-)，女，湖北洪湖人，博士，教授，研究方向为植物资源利用及观赏园艺。