ZHANG Na,PENG Ding-xiang,SUN Yu-hong,et al.Establishment of Cotyledon Explants Regeneration System in Seedless Watermelon ‘Mitong’[J].Northern Horticulture,2012,36(21):100-102.
无籽西瓜“蜜童”子叶再生体系的建立
- Title:
- Establishment of Cotyledon Explants Regeneration System in Seedless Watermelon ‘Mitong’
- 文章编号:
- 1001-0009（2012）21-0100-03
- Keywords:
- seedless watermelon; cotyledon; regeneration system
- 分类号:
- S 651
- 文献标志码:
- A
- 摘要:
- 以无籽西瓜“蜜童”无菌苗子叶为外植体，建立了高效的组培再生体系。结果表明：不定芽诱导培养基为MS+1.0 mg/L 6-BA+ 0.1 mg/L NAA，诱导率达88.33％，最佳伸长培养基为MS+0.2 mg/L 6-BA，最佳生根培养基为1/2MS+0.5 mg/L IBA，生根率达90％。
- Abstract:
- Cotyledon excised from seedlings of watermelon ‘Hitong’ were used as explants，an efficient plant regeneration system via organogenesis was established in triploid watermelon ‘Mitong’.The results showed that 88.33％ explants could regenerate to adventitious shoots on MS medium with 1.0 mg/L 6-BA and 0.1 mg/L NAA.The elongation media was MS+0.2 mg/L 6-BA.90％ adventitious shoots were successfully rooted on a 1/2MS medium with 0.5 mg/L IBA.
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备注/Memo
第一作者简介：张娜（1980-），女，在读博士，农艺师，研究方向为西瓜栽培与育种。E-mail:zn800329@163.com.