Dilraba·DILXAT,Muhrapiya·ARKIN,ZHANG Fu-chun,et al.Regeneration Plants of Cotyledon and Hypocotyl of Xinjiang Brassica rapa L.[J].Northern Horticulture,2012,36(24):132-135.
新疆恰玛古子叶及下胚轴植株再生体系的建立
- Title:
- Regeneration Plants of Cotyledon and Hypocotyl of Xinjiang Brassica rapa L.
- 文章编号:
- 1001-0009（2012）24-0132-04
- Keywords:
- Brassica rapa L.; cotyledon; hypocotyl; callus; regeneration plants
- 分类号:
- S 631.3
- 文献标志码:
- A
- 摘要:
- 以新疆恰玛古种子萌发5 d无菌苗的子叶及下胚轴为外植体，研究了各种不同激素配比条件下新疆恰玛古的再生体系。结果表明：培养基MS+2,4-D 2.0 mg/L+KT 0.1 mg/L对愈伤组织诱导效果较好，子叶、下胚轴的愈伤诱导率分别为65.4%、95.5%；再生芽的诱导培养基为6-BA 3.0 mg/L+NAA 0.5 mg/L+AgNO3 5.0 mg/L，子叶、下胚轴的芽分化率分别为20.8%、65.0%。将芽转入生根培养基MS+IBA 0.5 mg/L中诱导生根。20 d后，生根的小植株练苗移入花土中，成活率达95％。
- Abstract:
- The regeneration system of tissue culture of Xinjiang Brassica rapa L. was discussed by using cotyledon and hypocotyl of 5 day-old seedling of Xinjiang Brassica rapa.L as the explant.The results showed that MS+2,4-D 2.0 mg/L+KT 0.1 mg/L medium was effective for callus induced，the averaging callus frequences were 65.4%，95.5% for cotyledon，hypocotyl；shoots were induced with 6-BA 3.0 mg/L+NAA 0.5 mg/L+AgNO3 5.0 mg/L，the averaging regeneration shoots frequence was 20.8%，65.0% for cotyledon，hypocotyl；shoots was transferred into medium of MS+IBA 0.5 mg/L to root；after 20 days，regeneration plants were transferred into flower-soil and its survivable rate was more than 95%.
参考文献/References:
[1]
毛居代
,
帕热德姑丽·托合提
,
布海力期木·伊米提
.
芜菁种子中总黄酮提取工艺研究
[J].
种子
,2011,30(1):39-41.
[2]肖春霞,张洪亮.恰玛古膏影响中晚期大肠癌化疗相关指标的临床观察[J].亚太传统医药,2010,6(12):30-31. [3]马光,周波,李玉花.芜菁高频率再生体系的建立及优化[J].园艺学报,2008,35(6):833-840. [4]赵巧阳,赖钟雄.硝酸银在离体培养和转化中的作用及其机理[J].亚热带农业研究,2008,4(1):62-66. [5]MA G，GUO J P.Effects of thidiazuron and N6-benzylaminopurine on endogenous hormone levels during regeneration of Brassica campestrisssp.Rapifera[J].Journal of Zhejiang University,2010,36(3):237-245. [6]邢德峰,李新玲,王全伟,等.影响大白菜高效离体培养再生的因素[J].植物生理学通讯,2003,39(5):420-424. [7]黄俊轩,李双跃,李建科,等.花椰菜叶片离体再生技术的研究[J].天津农学院学报,2006,13(4):21-23. [8]李艳红,宋秀珍,庄木.青花菜组织培养再生体系的研究[J].首都师范大学学报,2001,22(3):48-53.
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备注/Memo
第一作者简介：地丽热巴?地里夏提（1985-），女，在读硕士，研究方向为生物化学与分子生物学。E-mail:dilraba8588@163.com.