LIU Ling-di,XIE Xiao-liang,WEN Chun-xiu,et al.rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge[J].Northern Horticulture,2012,36(20):146-148.
不同丹参种质的rDNA ITS序列分析
- Title:
- rDNA ITS Sequence Analysis of Different Germplasm of Salvia miltiorrhiza Bge
- 文章编号:
- 1001-0009（2012）20-0146-03
- 分类号:
- S 567
- 文献标志码:
- A
- 摘要:
- 为测定丹参核糖体DNA的ITS序列，研究比较了28份不同来源丹参（Salvia miltiorrhiza Bge）种质的ITS序列。结果表明：丹参rDNA的ITS1、ITS2及5.8S rDNA的完全序列（长度范围在600～619 bp之间），ITS1为227～228 bp，5.8S为166 bp，ITS2为206~224 bp；ITS区的变异主要发生在内转录间隔区ITS1和ITS2区，其中ITS1、ITS2区的变异位点为31和25，分别占各自区位点的13.5%和11.2%；ITS序列在丹参种内比较保守，有些丹参种质之间有较小的差异，ITS序列可以作为丹参种质分子鉴定的依据。
- Abstract:
- In order to study rDNA ITS sequence of Salvia，the rDNA ITS sequence of twenty-eight Salvia germplasm from different area were analyzed.The results showed that the length of rDNA ITS gene sequence of Salvia was 600~619 bp，including ITS1 (227~228 bp)，5.8S (166 bp) and ITS2 (206~224 bp).There were thirty-one variable sites in the ITS1 area (it was 13.5% of total site)，twenty-five variable sites in the ITS2 area (it was 11.2% of total site).The ITS1 and ITS2 gene fragments were conservative at specific level in Salvia，while there were less difference among some germplasm，so they could be molecular marker for authentication of Salvia.
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备注/Memo
第一作者简介：刘灵娣（1978-），女，河北定州人，博士，助理研究员，现主要从事药用植物栽培育种研究工作。E-mail:nkyliulingdi@126.com.