LIU Lei,HUANG Xiang,ZHONG Zhao-dong,et al.Study on Tissue Culture of Peony Sprout[J].Northern Horticulture,2012,36(20):115-116.
牡丹‘乌龙捧盛’鳞芽组织培养技术研究
- Title:
- Study on Tissue Culture of Peony Sprout
- 文章编号:
- 1001-0009(2012)20-0115-02
- Keywords:
- tree peony; sprout; tissue culture
- 分类号:
- S 685.110.36
- 文献标志码:
- B
- 摘要:
- 以牡丹鳞芽为试材,分别研究了不同暗处理时间和植物生长调节剂组合对鳞芽褐化与生根的影响。结果表明:在鳞芽初代培养过程中,先暗培养7 d,而后转入正常光照培养,可明显减轻鳞芽褐化;诱导根原基形成的最适培养基为:1/2MS+NAA 2.0 mg/L+IAA 2.0 mg/L+6-BA 0.1 mg/L或1/2MS+NAA 1.0 mg/L+IBA 1.0 mg/L+AC 0.2 g/L。
- Abstract:
- The effects of different dark treatments and plant growth regulators combination on browning and rooting were studied respectively.The results showed that during the course of initial culture,sprout was cultured under dark for 7 d,and then sprout was cultured under normal light condition,and browning was relieved obviously.The most optimum medium for root primordium was 1/2MS+NAA 2.0 mg/L+IAA 2.0 mg/L+6-BA 0.1 mg/L or 1/2MS+NAA 1.0 mg/L+IBA 1.0 mg/L+AC 0.2 g/L.
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备注/Memo
第一作者简介:刘磊(1983-),男,河南安阳人,硕士,助教,现主要从事花卉栽培生理和生物技术研究工作。E-mail:swuliulei@yahoo.com.cn.