ZHANG Xiao-li.,WANG Jian-jun,XU Shou-zhen,et al.Research on Callus Induction and Cell Suspension Culture of Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao[J].Northern Horticulture,2012,36(01):162-164.
怀地黄愈伤组织诱导及细胞悬浮培养体系的建立
- Title:
- Research on Callus Induction and Cell Suspension Culture of Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao
- 文章编号:
- 1001-0009(2012)01-0162-03
- Keywords:
- Rehmannia glutinosa f. hueichingensis (Chan et Sehih) Hsiao; callus; cell suspension culture
- 分类号:
- S 567.23+9
- 文献标志码:
- A
- 摘要:
- 研究了植物生长调节剂和接种方式对怀地黄叶片愈伤组织诱导的影响和细胞悬浮培养体系的建立。结果表明：将叶片正接在MS+2,4-D 1 mg/L+6-BA 0.4 mg/L培养基上，愈伤组织生长良好，诱导率达100%；在该培养基上多次继代，可形成3种类型的愈伤组织，其中Ⅰ型愈伤组织淡黄色、颗粒透亮、分散性好且疏松易脆，适合进行细胞悬浮培养；在MS+2,4-D 3 mg/L＋6-BA 0.5 mg/L培养基中，悬浮培养细胞生长曲线呈“S”型，培养15 d时细胞干重达最大值，为4.94 g/L。
- Abstract:
- The effects of inoculation method and plant growth regulator on callus induction and establishment of cell suspension culture system were studied. The rusults showed that when upper surface of leaf contact the medium, callus grows well on MS+2,4-D 1 mg/L+6-BA 0.4 mg/L, induction rate was 100%; on the same medium, callus could be divided into 3 types, callus of typeⅠwas straw yellow, bright particles, well dispersed and granular, fragile, this type of callus was suitable for cell suspension culture; on MS+2,4-D 3 mg/L＋6-BA 0.5 mg/L, the growth curve of suspension culture cells were ‘S’ type, cells dry weight was 4.94 g/L when cells cultured for 15 days.
参考文献/References:
[1]焦作市科学技术局.四大怀药[M].郑州:中原农民出版社,2004．
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备注/Memo
第一作者简介：张晓丽（1981-），女，河南漯河人，硕士，讲师，研究方向为药用植物生物技术。