LI Xuelong,LI Yue,ZHANG Peng,et al.Comparative Analysis of Main Agronomic Characters and Active Ingredients of Different Varieties of Ganoderma lucidum[J].Northern Horticulture,2022,(13):119-125.[doi:10.11937/bfyy.20214652]
不同灵芝菌株主要农艺性状及活性成分比较分析
- Title:
- Comparative Analysis of Main Agronomic Characters and Active Ingredients of Different Varieties of Ganoderma lucidum
- Keywords:
- Ganoderma lucidum; agronomic trait; active ingredients
- 文献标志码:
- A
- 摘要:
- 以国内8个主栽灵芝菌株为试材，采用灵芝菌株菌丝培养、段木栽培及灵芝子实体中主要活性成分含量检测等方法，比较及分析了灵芝菌株主要农艺性状及子实体活性成分含量，以期筛选获得适宜辽宁地区栽培的优质灵芝菌株。结果表明：不同灵芝菌株母种、原种、栽培种菌丝萌发活力、菌丝平均生长速度、菌丝长势、菌丝特征存在一定差异。在相同栽培条件下，不同菌株灵芝子实体单质量、菌柄长度、菌柄直径、菌盖直径、菌盖厚度、生物学效率等主要农艺性状均显示出一定差异，菌株006生物学效率最低，为9.83%，菌株003生物学效率最高，为16.10%。试验菌株所获得灵芝中三萜及甾醇、多糖等灵芝标志性活性成分含量亦有所不同，其含量均高于药典规定，菌株004三萜及甾醇含量比药典规定高出110.00%，菌株008多糖含量比药典规定高出43.33%。不同灵芝菌株子实体中蛋白质及16种氨基酸含量有所差异，蛋白质含量为5.10%~13.40%，16种氨基酸含量为2.69%~7.24%，为灵芝高效优质栽培选择灵芝菌株提供参考依据。
- Abstract:
- Eight main strains of Ganoderma lucidum in China were used as experimental materials，the main agronomic characters of Ganoderma lucidum strains and the content of active components in fruiting bodies were compared and analyzed by means of mycelial culture of Ganoderma lucidum strains, segment wood cultivation and detection of the content of main active components in Ganoderma lucidum fruiting bodies, in order to screen and obtain high-quality Ganoderma lucidum strains suitable for cultivation in Liaoning Province.The results showed that there were significant differences in mycelial germination activity，average growth rate and mycelial characteristics of stock culture，pre-culture spawn and spawn among different Ganoderma lucidum strains.The main agronomic characters of single weight of fruit body，stipe length，stipe diameter，pileus diameter，pileus thickness，biological efficiency of different strains under the same cultivation conditions were also different.The lowest biological efficiency was 9.83% got by strain 006，and the highest was 16.10% got by strain 003.The contents of triterpenoids，sterols and polysaccharides in Ganoderma lucidum obtained by the test strain were different，which were all higher than those prescribed by Chinese Pharmacopeias.The content of triterpenoids and sterols of strain 004 was 110.00% higher than those prescribed by Chinese Pharmacopeias.The content of polysaccharides of strain 008 was 43.33% higher than hose prescribed by Chinese Pharmacopeias.The contents of protein and 16 kinds of amino acids in fruit bodies of different Ganoderma lucidum strains were different，the contents of protein were 5.10%-13.40%，and the contents of 16 kinds of amino acids were 2.69%-7.24%.This study provided reference basis for selection of Ganoderma lucidum strains for efficient and high quality cultivation.
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备注/Memo
第一作者简介：李学龙（1984-），男，硕士，助理研究员，现主要从事食药用真菌高效栽培及天然活性产物等研究工作。E-mail：lixuelong2008@126.com.责任作者：张敏（1972-），女，博士，研究员，现主要从事食用菌遗传育种与栽培技术等研究工作。E-mail：zhangmindun@163.com.基金项目：辽宁省科技厅重点研发计划资助项目（2020JH2/10200013）；国家现代农业产业技术体系资助项目（CARS-20）；中央引导地方科技发展专项资金计划资助项目（2021JH6/10500133）。收稿日期：2021-11-16