ZHAO Zhan,HOU Shengcan,WANG Xiaoting,et al.Effects of Exogenous Substances on Physiological Property of Watermelon Seedling Under Low Temperature Stress[J].Northern Horticulture,2021,(18):29-35.[doi:10.11937/bfyy.20210508]
外源物质对低温胁迫下西瓜幼苗生理特性的影响
- Title:
- Effects of Exogenous Substances on Physiological Property of Watermelon Seedling Under Low Temperature Stress
- 文献标志码:
- A
- 摘要:
- 以‘B4’西瓜为试材，采用6个水杨酸（SA）浓度（0.01、0.1、0.5、1.0、2.5、5.0 mmol·L-1）和4个油菜素内酯（BR）浓度（0.005、0.05、0.5、5.0 mg·L-1）分别喷施西瓜幼苗的叶片，研究了低温胁迫条件下不同浓度SA和BR对西瓜幼苗生理特性的影响，以期能够阐明遭受冷害胁迫时外源激素类物质调节西瓜幼苗生长发育和提高幼苗耐寒性的生理作用机制，为外源物质在西瓜设施栽培中缓解冷害胁迫提供参考依据。结果表明：低温胁迫条件下，随着SA和BR浓度的增加，超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、可溶性蛋白质含量均有先增加后降低的趋势，丙二醛（MDA）含量的变化趋势均是先降低后升高，SA的浓度为0.5 mmol·L-1时处理效果最明显，BR的最佳处理浓度为0.05 mg·L-1。此外，SA和BR均能够在短期内显著增加低温胁迫下西瓜幼苗中SOD活性、POD活性和可溶性蛋白质含量，并显著降低MDA含量；但随着低温处理时间的延长，SA和BR处理的西瓜幼苗中SOD活性、POD活性和可溶性蛋白质含量均有降低的趋势，MDA含量有增加趋势，在低温处理的第0.5天，SA的处理效果最好，而BR是在第1.5天效果最好。
- Abstract:
- The watermelon variety ‘B4’ was selected as material to investigate the effect of salicylic acid (SA) and brassinolide (BR) on seedling physiological characteristics of watermelon under low temperature stress.Six different concentrations of SA (0.01、0.1、0.5、1.0、2.5、5.0 mmol·L-1) and four different concentrations of BR (0.005、0.05、0.5、5.0 mg·L-1) were set up to spray on the leaf surface，respectively.In order to elucidate the physiological mechanism of exogenous hormones regulating the growth and development and improving cold tolerance of watermelon seedlings under cold stress,and provide a technical referance for the rational use of SA and BR in the cultivation of watermelon under low temperature stress.The results indicated that the relative malondial dehyde(MDA) content of watermelon leaf decreased firstly and then increased，while the superoxide dismutase (SOD) activity，peroxidase (POD) activity and soluble protein content increased firsty and then decreased with the increase of SA and BR spraying concentrations under low temperature stress．The optimal concentrations of SA and BR to spray watermelon seedlings were 0.5 mmol·L-1and 0.05 mg·L-1，respectively.In addition，the SOD activity，POD activity and soluble protein content could be increased significantly，while the MDA content decreased significantly after a period of low temperature treatment.The SOD activity，POD activity and soluble protein content showed a trend of gradual decrease，and the MDA content increased gradually with the extension of low temperature treatment time.The treatment effect of SA was the best on day 0.5 of low temperature stress, while that of BR was the best on day 1.5.
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备注/Memo
第一作者简介：赵展（1987-），女，博士，讲师，现主要从事作物遗传育种等研究工作。E-mail：13525579383@163.com.责任作者：杨桦（1972-），女，本科，教授，现主要从事农业物联网等研究工作。E-mail：yanghua@kfu.edu.cn.基金项目：开封大学博士基金资助项目（KDBS-2017-003）；河南省高等学校重点科研项目计划支持（20B210013，21A520029，21A520028）；开封市科技发展计划（科技攻关）项目（2002007，2102009）；开封市政府决策研究资助项目（KFKTB2020-11）。收稿日期：2021-01-29