[1]罗智文,刘洋,焦云健,等.蜜环菌YN3862菌株的高密度发酵工艺[J].北方园艺,2020,44(20):124-129.[doi:10.11937/bfyy.20200419]
　LUO Zhiwen,LIU Yang,JIAO Yunjian,et al.High-density Fermentation Technology of Armillaria mellea YN3862 Strain[J].Northern Horticulture,2020,44(20):124-129.[doi:10.11937/bfyy.20200419]
点击复制

蜜环菌YN3862菌株的高密度发酵工艺

《北方园艺》[ISSN:1001-0009/CN:23-1247/S] 卷: 第44卷 期数: 2020年20 页码: 124-129 栏目: 出版日期: 2020-10-30
Title:
High-density Fermentation Technology of Armillaria mellea YN3862 Strain
作者:
罗智文1; 2; 刘洋3; 焦云健1; 2; 邓先余1; 2; 张棋麟1; 2; 林连兵1; 2
（1.昆明理工大学 生命科学与技术学院，云南 昆明 650500；2.云南省高校饲用抗生素替代技术工程研究中心，云南 昆明 650500；3.云南佳能达医药有限公司，云南 昆明 650106）
Author(s):
LUO Zhiwen1; 2; LIU Yang3; JIAO Yunjian1; 2; DENG Xianyu1; 2; ZHANG Qilin1; 2; LIN Lianbing1; 2
(1.Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500;2.Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College,Kunming,Yunnan 650500;3.Yunnan Jianengda Pharmaceutical Co.Ltd.,Kunming,Yunnan 650106)
关键词:
蜜环菌; 培养基; 液体发酵; 高密度培养
Keywords:
Armillaria mellea; culture medium; liquid fermentation; high-density culture
DOI:
10.11937/bfyy.20200419
文献标志码:
A
摘要:
以蜜环菌YN3862菌株为试材，采用固体培养和液体深层发酵相结合的方法，研究蜜环菌的最优母种培养基和最佳发酵条件，以期达到高密度培养蜜环菌目的。结果表明：该菌株最适母种培养基成分为木屑100 g?L-1，麦麸100 g?L-1，葡萄糖20 g?L-1，琼脂15 g?L-1，牛肉膏5 g?L-1；最佳液体培养基成分为葡萄糖1%，蔗糖2%，蚕蛹粉3%，MgSO4?7H2O 0.075%，KH2PO4 0.15%，乙醇1%；液体深层发酵采用200 L发酵罐，装入100 L液体培养基，以5%接种量接入匀质后的液体菌种，发酵温度25 ℃，溶解氧含量（DO）为55%左右，在发酵的0~24、24~36、36~48、48~144 h，转速分别设置为60、80、120、150 r?min-1，在发酵时间为120 h时，生物量(干质量）最高，达到2.5 g?(100mL)-1。因此，采用上述培养基和液体深层发酵工艺，产出的生物量大，菌丝球活力好，实现了高密度培养。
Abstract:
Armillaria mellea YN3862 strain was used as test material, a combination of solid culture and deep liquid fermentation was used to study the optimal mother culture medium and fermentation conditions of A. mellea, in order to achieve the purpose of high-density culture of A. mellea.The results showed that the best culture medium for A.mellea includes sawdust 100 g?L-1,wheat bran 100 g?L-1,glucose 20 g?L-1,agar 15 g?L-1 and beef extract 5 g?L-1.The optimum liquid medium composition was 1% glucose,2% sucrose,3% silkworm pupa powder,0.075% magnesium sulfate heptahydrate,0.15% potassium dihydrogen phosphate and 1% ethanol.Deep liquid fermentation was conducted in a 200 L fermentation tank and filled with 100 L liquid culture medium,the liquid strain was inoculated with 5% inoculation amount,the fermentation temperature was 25 ℃ and the dissolved oxygen was about 55%.At 0-24,24-36,36-48,48-144 hours of fermentation,the rotational speeds were set as 60,80,120,150 r?min-1,respectively.The optimal fermentation time was 120 hours.At this time,the mycelium quantity could reach 2.5 g?(100mL)-1.In conclusion,by using the above medium and deep liquid fermentation technology,the biomass produced was large,and the produced mycelium pellet had good vitality and realized high-density culture.

参考文献/References:

[1]潘崇环.珍稀食用菌栽培与名贵野生菌的开发利用[M].北京:中国农业出版社,2004.[2]王鑫,亢学平,胡志强,等.蜜环菌原种配方筛选试验[J].中国林副特产,2017(6):41-42.[3]李福后,王伟霞.5株蜜环菌产几种胞外酶活性比较[J].淮海工学院学报(自然科学版),2006,15(3):58-61.[4]吴兴亮,连宾,邹方伦.贵州蜜环菌资源及其生态研究[J].贵州科学,2003,21(3):56-60.[5]NAGAI K,TANAKA J,KIHO T,et al.Synthesis and antitumor activities of mitomycin C (1→3)-β-D-glucan conjugate[J].Chem Pharma Bulletin,1992,40(4):986-989.[6]KIHO T,SHIOSE Y,NAGAI K,et al.Polysaccharides in fungi.XXIX.Structural features of two antitumor polysaccharides from the fruiting bodies of Armillariella tabescens[J].Chem Pharma Bulletin,1992,40(8):2212.[7]OTA Y,INTINI M,HATTORI T.Genetic characterization of heterothallic and non-heterothallic Armillaria mellea sensu stricto[J].Mycological Res,2000,104(9):1046-1054.[8]顾雅君,张明艳,唐宏亮,等.蜜环菌和天麻、猪苓的关系及尚未大量人工驯化原因[J].中国食用菌,2008,27(6):20,28.[9]郭顺星.蜜环菌和猪苓的发育学及其二者的相互作用机理[D].北京:中国协和医科大学,1995.[10]黄靖雯,赖长江生,袁媛,等.猪苓与蜜环菌化学成分研究的相关分析进展[J].中国中药杂志，2017,42(15):2905-2914.[11]唐德华,廖文菊.天麻蜜环菌研究综述[J].安徽农业科学,2015,43(13):31-32.[12]李露,王思爽,马丽颖,等.蜜环菌的活性成分和药用价值[J].吉林医药学院学报,2018,39(4):307-310.[13]OBUCHI T,KONDOH H,WATANABE N,et al.Armillaric acid,a new antibiotic produced by Armillaria mellea[J].Planta Med,1990,56:198-201.[14]王惠国,冯宝民.蜜环菌多糖免疫调节活性的实验研究[J].陕西科技大学学报,2009,27(2):62-64.[15]沈明花,金梅花.密环菌多糖对小鼠急性肝损伤的保护作用[J].食品研究与开发,2014(3):91-93.[16]闫军,王欢,孙冬梅.蜜环菌培养条件优化[J].黑龙江农业科学,2016(3):112-114.[17]陈顺方,祁岑,黄先敏.蜜环菌的生产技术[J].现代农业科技,2009(14):125-127.[18]黄燕芬,唐丽,范成五.蜜环菌原种培养基组分筛选[J].贵州农业科学,2005,33(4):54-55.[19]唐兴国,王伯诚,赖小芳.天麻共生蜜环菌液体种培养基的优化[J].浙江农业科学,2017,58(4):635-637.[20]亢学平,王鑫,胡志强,等.伴栽天麻蜜环菌的适宜培养基筛选[J].食药用菌,2016,24(2):104-105.[21]刘太林,李黎,周正涛,等.六种蜜环菌固体培养基配方的筛选[J].北方园艺,2015（18）:145-148.[22]牛晓静,陈天朝,鲁静,等.槐角中还原糖的含量测定[J].中医药信息,2019(6):18-21.[23]袁媛,刘景圣.蜜环菌液体深层发酵培养基的优选[J].农产品加工(学刊),2009(7):57-59.[24]熊泽,邵伟,乐超银,等.蜜环菌液体发酵条件研究及其饮料开发[J].三峡大学学报(自然科学版),2003,25(6):565-567.[25]邵伟,乐超银,刘世玲,等.蜜环菌深层液体发酵条件研究[J].中国食用菌,2004,23(3):50-51.[26]刘宇,龚思慧,彭楠,等.蜜环菌液态发酵培养基及补料分批发酵工艺的优化[J].食品与发酵工业,2016,42(5):187-191.[27]张颖,沈业寿.液体发酵蜜环菌培养条件的优化及菌体多糖的分离[J].食用菌,2001(2):5-7.[28]程显好,刘林德,董洪新,等.蜜环菌菌丝体液体培养条件的优化[J].中药材,2007,30(5):509-512.[29]贺建超,李利军,卢美欢,等.液体培养富硒蜜环菌的研究[J].陕西农业科学,2018,64(4):33-35.[30]唐兴国,王伯诚,赖小芳.天麻共生蜜环菌液体种培养基的优化[J].浙江农业科学,2017,58(4):635-637.

相似文献/References:

[1]于海燕,陈刚,建德锋,等.毛花猕猴桃组培苗增殖扩繁培养基筛选试验[J].北方园艺,2013,37(04):111.
[2]马紫英,夏 斌,倪 焱,等.以椰子壳为主碳源的巨大口蘑原种培养基优化[J].北方园艺,2014,38(13):142.
　MA Zi-ying,XIA Bin,NI Yan,et al.Optimization of Culture Medium for Second-Class Spawn of Tricholoma giganteum Using Coconut Shell as Main Carbon Source[J].Northern Horticulture,2014,38(20):142.
[3]郑 晟,邓 赞,张凌云.大白杜鹃种子无菌萌发及幼苗生长条件研究[J].北方园艺,2014,38(11):77.
　ZHENG Sheng,DENG Zan,ZHANG Ling-yun.Research on Seeds Aseptic Germination and Seedling Growth Condition of Rhododendron decorum Franch[J].Northern Horticulture,2014,38(20):77.
[4]朱会霞.灵芝真菌发酵产灵芝酸培养基优化研究[J].北方园艺,2013,37(22):154.
　ZHU Hui-xia.Study on the Optimization of Culture Medium of Ganoderma Fermentation Before Extraction of Ganoderic Acid[J].Northern Horticulture,2013,37(20):154.
[5]唐业刚,崔 伟.宜昌百合鳞茎增殖诱导条件研究[J].北方园艺,2014,38(02):112.
　TANG Ye-gang,CUI Wei.Research on Proliferation Culture Condition of Lilium leucanthum Baker Bulb[J].Northern Horticulture,2014,38(20):112.
[6]郭欣欣,樊淑华,王永立.金针菇液体培养基的优化研究[J].北方园艺,2014,38(02):146.
　GUO Xin-xin,FAN Shu-hua,WANG Yong-li.Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes [J].Northern Horticulture,2014,38(20):146.
[7]郭朋伟,高晔华,高日,等.“贝吉佳”草莓脱毒苗增殖的研究[J].北方园艺,2013,37(10):110.
　GUO Peng-wei,GAO Ye-hua,GAO Ri,et al.Study on Proliferation of Virusfree Strawberry ‘Bechika’[J].Northern Horticulture,2013,37(20):110.
[8]周慧杰.灰树花的母种及原种培养基筛选研究[J].北方园艺,2013,37(14):155.
　ZHOU Hui-jie.Study on Selection of Mother and Original Culture Media of Grifora frondosa[J].Northern Horticulture,2013,37(20):155.
[9]杨丽维,王玉,班立桐,等.杏鲍菇液体菌种培养基的筛选和优化[J].北方园艺,2014,38(06):150.
　YANG Li-wei,WANG Yu,BAN Li-tong,et al.Study on the Screening and Optimizing of Liquid Spawn Medium of Pleurotus eryngii[J].Northern Horticulture,2014,38(20):150.
[10]忻龙祚,金亚征,苗文静.蒙古口蘑菌丝的室内优化培养研究[J].北方园艺,2013,37(11):140.
　XIN Long-zuo,JIN Ya-zheng,MIAO Wen-jing.Study on Optimization of Mycelia Culture of Mongolia Mushroom from Indoor[J].Northern Horticulture,2013,37(20):140.
[11]温钢,刘海燕,杨梅.蜜环菌胞外多糖液体发酵培养基的优化[J].北方园艺,2013,37(19):152.
　WEN Gang,LIU Hai-yan,YANG Mei.Optimization of Liquid Culture Medium of Armillaria mellea Exopolysaccharides[J].Northern Horticulture,2013,37(20):152.
[12]任思竹,郭亚萍,张国庆,等.蜜环菌菌种及配方的筛选[J].北方园艺,2013,37(18):145.
　RENSi-zhu,GUOYa-ping,ZHANGGuo-qing,et al.ScreeningonSpeciesandFormulationofArmillariamelleaStrains[J].Northern Horticulture,2013,37(20):145.

备注/Memo

第一作者简介：罗智文（1996-），男，硕士研究生，研究方向为微生物高密度发酵及生物防治。E-mail:717570821@qq.com.责任作者：张棋麟（1988-），男，博士，副教授，现主要从事生态基因组学等研究工作。E-mail:zhangql@kust.edu.cn.基金项目：国家自然科学基金资助项目（31760042）；天麻两菌工厂生产技术开发校企合作资助项目（2019FW047）。收稿日期：2020-02-07

更新日期/Last Update: 2021-01-13